scholarly journals Label-free single-cell protein quantification using a drop-based mix-and-read system

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Alireza Abbaspourrad ◽  
Huidan Zhang ◽  
Ye Tao ◽  
Naiwen Cui ◽  
Haruichi Asahara ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Protein Quantification ◽  
Cell Protein ◽  
Label Free

scholarly journals Multiplexed single-cell proteomics using SCoPE2

2021 ◽  
Author(s):  
Aleksandra A Petelski ◽  
Edward Emmott ◽  
Andrew Leduc ◽  
R. Gray Huffman ◽  
Harrison Specht ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Cost Effective ◽  
Protein Analysis ◽  
Single Cell Protein ◽  
Protein Quantification ◽  
Cell Protein ◽  
Label Free ◽  
Cost Effective Method

Many biological systems are composed of diverse single cells. This diversity necessitates functional and molecular single-cell analysis. Single-cell protein analysis has long relied on affinity reagents, but emerging mass-spectrometry methods (either label-free or multiplexed) have enabled quantifying over 1,000 proteins per cell while simultaneously increasing the specificity of protein quantification. Isobaric carrier based multiplexed single-cell proteomics is a scalable, reliable, and cost-effective method that can be fully automated and implemented on widely available equipment. It uses inexpensive reagents and is applicable to any sample that can be processed to a single-cell suspension. Here we describe an automated Single Cell ProtEomics (SCoPE2) workflow that allows analyzing about 200 single cells per 24 hours using only standard commercial equipment. We emphasize experimental steps and benchmarks required for achieving quantitative protein analysis.

scholarly journals Single-cell protein profiling in microchambers with barcoded beads

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Lucas Armbrecht ◽  
Rafael Sebastian Müller ◽  
Jonas Nikoloff ◽  
Petra Stephanie Dittrich
Keyword(s):  
Single Cell ◽  
Cell Lines ◽  
Single Cells ◽  
Single Cell Protein ◽  
Protein Quantification ◽  
Protein Profiling ◽  
Cell Protein ◽  
Breast Cancer Cell Lines ◽  
Mammalian Cell Lines ◽  
Galectin 3

Abstract Single-cell profiling provides insights into cellular behaviour that macroscale cell cultures and bulk measurements cannot reveal. In the context of personalized cancer treatment, the profiling of individual tumour cells may lead to higher success rates for therapies by rapidly selecting the most efficacious drugs. Currently, genomic analysis at the single-cell level is available through highly sensitive sequencing approaches. However, the identification and quantification of intracellular or secreted proteins or metabolites remains challenging. Here, we introduce a microfluidic method that facilitates capture, automated data acquisition and the multiplexed quantification of proteins from individual cells. The microfluidic platform comprises 1026 chambers with a volume of 152 pL each, in which single cells and barcoded beads are co-immobilized. We demonstrated multiplexed single-cell protein quantification with three different mammalian cell lines, including two model breast cancer cell lines. We established on-chip immunoassays for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), galectin-3 (Gal-3) and galectin-3 binding protein (Gal-3bp) with detection limits as low as 7.0 × 104, 2.3 × 105 and 1.8 × 103 molecules per cell, respectively. The three investigated cell types had high cytosolic levels of GAPDH and could be clearly differentiated by their expression levels of Gal-3 and Gal-3bp, which are important factors that contribute to cancer metastasis. Because it employed commercially available barcoded beads for this study, our platform could be easily used for the single-cell protein profiling of several hundred different targets. Moreover, this versatile method is applicable to the analysis of bacteria, yeast and mammalian cells and nanometre-sized lipid vesicles.

Single cell protein as food and feed

Nahrung/Food ◽  
1988 ◽  
Vol 32 (3) ◽  
pp. 219-229 ◽  
Author(s):  
A. Giec ◽  
J. Skupin
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Food And Feed

Single-Cell Protein

1984 ◽  
Vol 2 (1) ◽  
pp. 285-311 ◽  
Author(s):  
R.B. Vasey ◽  
K.A. Powell
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Cell Protein

Production of single cell protein material

1984 ◽  
Vol 4 (6) ◽  
pp. 1050
Author(s):  
Emil A Malick ◽  
John W Vanderveen ◽  
Donald O
Keyword(s):  
Single Cell ◽  
Single Cell Protein ◽  
Cell Protein
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