scholarly journals A computer-designed scaffold for bone regeneration within cranial defect using human dental pulp stem cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Doo Yeon Kwon ◽  
Jin Seon Kwon ◽  
Seung Hun Park ◽  
Ji Hun Park ◽  
So Hee Jang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Cranial Defect ◽  
Human Dental Pulp

scholarly journals Bone regeneration by human dental pulp stem cells using a helioxanthin derivative and cell-sheet technology

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Yasuyuki Fujii ◽  
Yoko Kawase-Koga ◽  
Hironori Hojo ◽  
Fumiko Yano ◽  
Marika Sato ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Cell Sheet ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp ◽  
Cell Sheet Technology

scholarly journals Decellularized bone extracellular matrix and human dental pulp stem cells as a construct for bone regeneration

2017 ◽  
Vol 28 (8) ◽  
pp. 730-748 ◽  
Author(s):  
Francesco Paduano ◽  
Massimo Marrelli ◽  
Noura Alom ◽  
Mahetab Amer ◽  
Lisa J. White ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Gelatin-biofermentative unsulfated glycosaminoglycans semi-interpenetrating hydrogels via microbial-transglutaminase crosslinking enhance osteogenic potential of dental pulp stem cells

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Annalisa La Gatta ◽  
Virginia Tirino ◽  
Marcella Cammarota ◽  
Marcella La Noce ◽  
Antonietta Stellavato ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Biological Response ◽  
Dental Pulp Stem Cells ◽  
Microbial Transglutaminase ◽  
Osteogenic Potential ◽  
Gelatin Matrix ◽  
Human Dental Pulp ◽  
Bone Deposition

Abstract Gelatin hydrogels by microbial-transglutaminase crosslinking are being increasingly exploited for tissue engineering, and proved high potential in bone regeneration. This study aimed to evaluate, for the first time, the combination of enzymatically crosslinked gelatin with hyaluronan and the newly developed biotechnological chondroitin in enhancing osteogenic potential. Gelatin enzymatic crosslinking was carried out in the presence of hyaluronan or of a hyaluronan–chondroitin mixture, obtaining semi-interpenetrating gels. The latter proved lower swelling extent and improved stiffness compared to the gelatin matrix alone, whilst maintaining high stability. The heteropolysaccharides were retained for 30 days in the hydrogels, thus influencing cell response over this period. To evaluate the effect of hydrogel composition on bone regeneration, materials were seeded with human dental pulp stem cells and osteogenic differentiation was assessed. The expression of osteocalcin (OC) and osteopontin (OPN), both at gene and protein level, was evaluated at 7, 15 and 30 days of culture. Scanning electron microscopy (SEM) and two-photon microscope observations were performed to assess bone-like extracellular matrix (ECM) deposition and to observe the cell penetration depth. In the presence of the heteropolysaccharides, OC and OPN expression was upregulated and a higher degree of calcified matrix formation was observed. Combination with hyaluronan and chondroitin improved both the biophysical properties and the biological response of enzymatically crosslinked gelatin, fastening bone deposition.

Chrysin induces osteogenic differentiation of human dental pulp stem cells

2021 ◽  
Vol 400 (2) ◽  
pp. 112466
Author(s):  
J.F. Huo ◽  
M.L. Zhang ◽  
X.X. Wang ◽  
D.H. Zou
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Methylglyoxal-Dependent Glycative Stress Is Prevented by the Natural Antioxidant Oleuropein in Human Dental Pulp Stem Cells through Nrf2/Glo1 Pathway

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 716
Author(s):  
Simona Delle Delle Monache ◽  
Fanny Pulcini ◽  
Roberta Frosini ◽  
Vincenzo Mattei ◽  
Vincenzo Nicola Talesa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Great Promise ◽  
Oral Diseases ◽  
Bioactive Component ◽  
Glycation End Products ◽  
Abnormal Accumulation ◽  
Synthetic Agents ◽  
Human Dental Pulp

Methylglyoxal (MG) is a potent precursor of glycative stress (abnormal accumulation of advanced glycation end products, AGEs), a relevant condition underpinning the etiology of several diseases, including those of the oral cave. At present, synthetic agents able to trap MG are known; however, they have never been approved for clinical use because of their severe side effects. Hence, the search of bioactive natural scavengers remains a sector of strong research interest. Here, we investigated whether and how oleuropein (OP), the major bioactive component of olive leaf, was able to prevent MG-dependent glycative stress in human dental pulp stem cells (DPSCs). The cells were exposed to OP at 50 µM for 24 h prior to the administration of MG at 300 µM for additional 24 h. We found that OP prevented MG-induced glycative stress and DPSCs impairment by restoring the activity of Glyoxalase 1 (Glo1), the major detoxifying enzyme of MG, in a mechanism involving the redox-sensitive transcription factor Nrf2. Our results suggest that OP holds great promise for the development of preventive strategies for MG-derived AGEs-associated oral diseases and open new paths in research concerning additional studies on the protective potential of this secoiridoid.

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