scholarly journals Erratum: Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Takeshi Sakurai ◽  
Hidefumi Mitsuno ◽  
Akihisa Mikami ◽  
Keiro Uchino ◽  
Masashi Tabuchi ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Receptor Gene ◽  
Pheromone Response ◽  
Targeted Disruption ◽  
Pheromone Receptor ◽  
Single Sex

scholarly journals Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Takeshi Sakurai ◽  
Hidefumi Mitsuno ◽  
Akihisa Mikami ◽  
Keiro Uchino ◽  
Masashi Tabuchi ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Sexual Behaviour ◽  
Receptor Gene ◽  
Transcription Activator ◽  
Pheromone Receptor ◽  
Behavioural Responses ◽  
Sensilla Trichodea ◽  
Moth Species ◽  
Species Specific

Abstract Male moths use species-specific sex pheromones to identify and orientate toward conspecific females. Odorant receptors (ORs) for sex pheromone substances have been identified as sex pheromone receptors in various moth species. However, direct in vivo evidence linking the functional role of these ORs with behavioural responses is lacking. In the silkmoth, Bombyx mori, female moths emit two sex pheromone components, bombykol and bombykal, but only bombykol elicits sexual behaviour in male moths. A sex pheromone receptor BmOR1 is specifically tuned to bombykol and is expressed in specialized olfactory receptor neurons (ORNs) in the pheromone sensitive long sensilla trichodea of male silkmoth antennae. Here, we show that disruption of the BmOR1 gene, mediated by transcription activator-like effector nucleases (TALENs), completely removes ORN sensitivity to bombykol and corresponding pheromone-source searching behaviour in male moths. Furthermore, transgenic rescue of BmOR1 restored normal behavioural responses to bombykol. Our results demonstrate that BmOR1 is required for the physiological and behavioural response to bombykol, demonstrating that it is the receptor that mediates sex pheromone responses in male silkmoths. This study provides the first direct evidence that a member of the sex pheromone receptor family in moth species mediates conspecific sex pheromone information for sexual behaviour.

scholarly journals A Single Sex Pheromone Receptor Determines Chemical Response Specificity of Sexual Behavior in the Silkmoth Bombyx mori

PLoS Genetics ◽  
2011 ◽  
Vol 7 (6) ◽  
pp. e1002115 ◽  
Author(s):  
Takeshi Sakurai ◽  
Hidefumi Mitsuno ◽  
Stephan Shuichi Haupt ◽  
Keiro Uchino ◽  
Fumio Yokohari ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Sex Pheromone ◽  
Bombyx Mori ◽  
Pheromone Receptor ◽  
Single Sex ◽  
Chemical Response ◽  
Response Specificity

Targeted disruption of the mouse colony-stimulating factor 1 receptor gene results in osteopetrosis, mononuclear phagocyte deficiency, increased primitive progenitor cell frequencies, and reproductive defects

Blood ◽  
2002 ◽  
Vol 99 (1) ◽  
pp. 111-120 ◽  
Author(s):  
Xu-Ming Dai ◽  
Gregory R. Ryan ◽  
Andrew J. Hapel ◽  
Melissa G. Dominguez ◽  
Robert G. Russell ◽  
...  
Keyword(s):  
Receptor Gene ◽  
Mononuclear Phagocyte ◽  
Proliferative Potential ◽  
Colony Stimulating Factor ◽  
Hematopoietic Tissue ◽  
Targeted Disruption ◽  
Tissue Macrophage ◽  
Stimulating Factor

The effects of colony-stimulating factor 1 (CSF-1), the primary regulator of mononuclear phagocyte production, are thought to be mediated by the CSF-1 receptor (CSF-1R), encoded by the c-fms proto-oncogene. To investigate the in vivo specificity of CSF-1 for the CSF-1R, the mouse Csf1r gene was inactivated. The phenotype ofCsf1−/Csf1r− mice closely resembled the phenotype of CSF-1-nullizygous(Csf1op/Csf1op) mice, including the osteopetrotic, hematopoietic, tissue macrophage, and reproductive phenotypes. Compared with their wild-type littermates, splenic erythroid burst-forming unit and high-proliferative potential colony-forming cell levels in bothCsf1op/Csf1op andCsf1−/Csf1r− mice were significantly elevated, consistent with a negative regulatory role of CSF-1 in erythropoiesis and the maintenance of primitive hematopoietic progenitor cells. The circulating CSF-1 concentration inCsf1r−/Csf1r− mice was elevated 20-fold, in agreement with the previously reported clearance of circulating CSF-1 by CSF-1R–mediated endocytosis and intracellular destruction. Despite their overall similarity, several phenotypic characteristics of theCsf1r−/Csf1r− mice were more severe than those of theCsf1op/Csf1op mice. The results indicate that all of the effects of CSF-1 are mediated via the CSF-1R, but that subtle effects of the CSF-1R could result from its CSF-1–independent activation.

scholarly journals Species-Specific Duplication and Adaptive Evolution of a Candidate Sex Pheromone Receptor Gene in Weather Loach

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1845
Author(s):  
Lei Zhong ◽  
Weimin Wang ◽  
Xiaojuan Cao
Keyword(s):  
Sex Pheromone ◽  
Adaptive Evolution ◽  
Olfactory Epithelium ◽  
Stop Codon ◽  
Courtship Behavior ◽  
Receptor Gene ◽  
Sequence Length ◽  
Premating Isolation ◽  
Pheromone Receptor ◽  
Species Specific

The release and sensation of sex pheromone play a role in the reproductive success of vertebrates including fish. Previous studies have shown that the weather loach Misgurnus anguillicaudatus perceives sex pheromones by olfaction to stimulate courtship behavior. It was speculated that weather loaches use smell to recognize intraspecific mates. However, the identification of loach pheromone receptor has not been reported. By comparative transcriptomic approach, we found that the olfactory receptor gene or114-1 was male-biasedly expressed in the olfactory epithelium of M. anguillicaudatus, M. bipartitus and the closely related species Paramisgurnus dabryanus. This sex-biased expression pattern implicated that or114-1 presumably encoded a sex pheromone receptor in loaches. M. bipartitus and P. dabryanus, like zebrafish, possess one or114-1 only. However, in M. anguillicaudatus, or114-1 has two members: Ma_or114-1a and Ma_or114-1b. Ma_or114-1a, not Ma_or114-1b, showed sex-differential expression in olfactory epithelium. Ma_or114-1b has base insertions that delayed the stop codon, causing the protein sequence length to be extended by 8 amino acids. Ma_or114-1a was subject to positive selection resulting in adaptive amino acid substitutions, which indicated that its ligand binding specificity has probably changed. This adaptive evolution might be driven by the combined effects of sexual selection and reinforcement of premating isolation between the sympatric loach species.

1879-P: Targeted Disruption of Muscle-Specific ROCK2 Results in Insulin Resistance In Vivo

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1879-P
Author(s):  
HYUN JEONG KIM ◽  
RODRIGO M. PEREIRA ◽  
AYKUT G. UNER ◽  
HYON LEE ◽  
YOUNG-BUM KIM
Keyword(s):  
Insulin Resistance ◽  
Targeted Disruption

Modulation of epidermal growth factor receptor proto-oncogene transcription by a promoter site sensitive to S1 nuclease

1988 ◽  
Vol 8 (10) ◽  
pp. 4174-4184
Author(s):  
A C Johnson ◽  
Y Jinno ◽  
G T Merlino
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Egf Receptor ◽  
Receptor Gene ◽  
Gene Promoter ◽  
Direct Repeat ◽  
S1 Nuclease ◽  
Epidermal Growth ◽  
Specific Factors

The epidermal growth factor (EGF) receptor is the functional target of the mitogen EGF and the cellular homolog of the avian erythroblastosis virus erbB oncogene product. Regulation of expression of the proto-oncogene encoding the EGF receptor can be elucidated by studying the structure and function of the gene promoter outside the confines of the cell. Previously, we reported the isolation of the human EGF receptor gene promoter. The promoter is highly GC rich, contains no TATA or CAAT box, and has multiple transcription start sites. An S1 nuclease-sensitive site has now been found 80 to 110 base pairs (bp) upstream from the major in vivo transcription initiation site. Two sets of direct repeat sequences were found in this area; both conform to the motif TCCTCCTCC. When deletion mutations were made in this region of the promoter by using either Bal 31 exonuclease or S1 nuclease, we found that in vivo activity dropped three- to fivefold, on the basis of transient-transfection analysis. Examination of nuclear protein binding to normal and mutated promoter DNAs by gel retardation analysis and DNase I footprinting revealed that two specific factors bind to the direct repeat region but cannot bind to the S1 nuclease-mutated promoter. One of the specific factors is the transcription factor Sp1. The results suggest that these nuclear trans-acting factors interact with the S1 nuclease-sensitive region of the EGF receptor gene promoter and either directly or indirectly stimulate transcription.

Sex chiniaerism and germ cell distribution in a series of chimaeric mice

Development ◽  
1975 ◽  
Vol 33 (1) ◽  
pp. 205-216
Author(s):  
Anne McLaren
Keyword(s):  
Bone Marrow ◽  
Blood Cells ◽  
Chromosome Analysis ◽  
Chromosome Morphology ◽  
Mixed Population ◽  
Cell Distribution ◽  
Single Sex ◽  
Xx Males

1. Of 30 mice born from aggregation of embryos from a multiple recessive strain with F1 embryos carrying the contrasting alleles, 4 females and 20 males proved to be overtly chimaeric. 2. Three XX/XX females, five XY/XY males and eight XY/XX males were identified by chromosome analysis. Thus 50 % of the population analysed were sex chimaeras, and all of these developed as phenotypic males, though one showed evidence of hermaphroditism. 3. In seven XY/XX chimaeras that bred, the genetic component undergoing spermatogenesis coincided in every case with the component identified by chromosome morphology as XY. 4. The F1 component predominated in metaphase plates derived from cultured blood cells. Comparison with direct preparations from bone marrow suggested selection in favour of F1 cells, either through differential proliferation of stem cells in vivo or differential response to phytohaemagglutinin in vitro. 5. In XY/XX males, the percentage of XX cells detected varied from 1 % to 98 % in blood, and from 0 % to 80 % in bone marrow. 6. Of eight ‘single-sex’ chimaeras progeny-tested (three XX/XX, five XY/XY), only one showed evidence of a mixed population of germ cells. The proportion of the two types of progeny varied significantly from litter to litter, but was unrelated to the age of the male.

The pAD1 Sex Pheromone Response in Enterococcus faecalis

1997 ◽  
pp. 1037-1040 ◽  
Author(s):  
S. Fujimoto ◽  
M. Bastos ◽  
K. Tanimoto ◽  
F. An ◽  
K. Wu ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Enterococcus Faecalis ◽  
Pheromone Response
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