scholarly journals Hybrid male sterility and genome-wide misexpression of male reproductive proteases

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Suzanne Gomes ◽  
Alberto Civetta
Keyword(s):  
Male Sterility ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Genome Wide

scholarly journals Genome-wide misexpression of X-linked versus autosomal genes associated with hybrid male sterility

2010 ◽  
Vol 20 (8) ◽  
pp. 1097-1102 ◽  
Author(s):  
X. Lu ◽  
J. A. Shapiro ◽  
C.-T. Ting ◽  
Y. Li ◽  
C. Li ◽  
...  
Keyword(s):  
Male Sterility ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Genome Wide

scholarly journals Rampant Misexpression in a Mimulus (Monkeyflower) Introgression Line Caused by Hybrid Sterility, Not Regulatory Divergence

2020 ◽  
Vol 37 (7) ◽  
pp. 2084-2098 ◽  
Author(s):  
Rachel E Kerwin ◽  
Andrea L Sweigart
Keyword(s):  
Male Sterility ◽  
Gene Expression Regulation ◽  
Hybrid Sterility ◽  
Introgression Line ◽  
Transcript Abundance ◽  
Stabilizing Selection ◽  
Hybrid Male ◽  
Specific Expression ◽  
Hybrid Male Sterility ◽  
Genome Wide

Abstract Divergence in gene expression regulation is common between closely related species and may give rise to incompatibilities in their hybrid progeny. In this study, we investigated the relationship between regulatory evolution within species and reproductive isolation between species. We focused on a well-studied case of hybrid sterility between two closely related yellow monkeyflower species, Mimulus guttatus and Mimulus nasutus, that is caused by two epistatic loci, hybrid male sterility 1 (hms1) and hybrid male sterility 2 (hms2). We compared genome-wide transcript abundance across male and female reproductive tissues (i.e., stamens and carpels) from four genotypes: M. guttatus, M. nasutus, and sterile and fertile progeny from an advanced M. nasutus–M. guttatus introgression line carrying the hms1–hms2 incompatibility. We observed substantial variation in transcript abundance between M. guttatus and M. nasutus, including distinct but overlapping patterns of tissue-biased expression, providing evidence for regulatory divergence between these species. We also found rampant genome-wide misexpression, but only in the affected tissues (i.e., stamens) of sterile introgression hybrids carrying incompatible alleles at hms1 and hms2. Examining patterns of allele-specific expression in sterile and fertile introgression hybrids, we found evidence for interspecific divergence in cis- and trans-regulation, including compensatory cis–trans mutations likely to be driven by stabilizing selection. Nevertheless, species divergence in gene regulatory networks cannot explain the vast majority of the gene misexpression we observe in Mimulus introgression hybrids, which instead likely manifests as a downstream consequence of sterility itself.

scholarly journals Genome wide screen reveals a specific interaction between autosome and X that is essential for hybrid male sterility

2018 ◽  
Author(s):  
Zhongying Zhao ◽  
Yu Bi ◽  
Xiaoliang Ren ◽  
Runsheng Li ◽  
Qiutao Ding ◽  
...  
Keyword(s):  
Male Sterility ◽  
Male Fertility ◽  
Hybrid Male ◽  
Female Progeny ◽  
Hybrid Male Sterility ◽  
Male And Female ◽  
Genome Wide ◽  
F1 Progeny ◽  
Asymmetric Hybrid ◽  
Parent Of Origin

Hybrid male progeny from interspecies cross are more prone to sterility or inviability than hybrid female progeny, and the male sterility and inviability often demonstrate a parent-of-origin asymmetry. However, the underlying mechanism of asymmetric sterility or inviability remains elusive. We previously established a genome-wide hybrid incompatibility (HI) landscape between Caenorhabditis briggsae and C. nigoni by phenotyping a large collection of C. nigoni strains each carrying a C. briggsae introgression. In this study, we investigate the genetic mechanism of asymmetric sterility and inviability in both hybrid male and female progeny between the two species. Specifically, we performed reciprocal crosses between C. briggsae and different C. nigoni strains that each carries a GFP-labeled C. briggsae genomic fragment referred to as introgression, and scored the HI phenotypes in the F1 progeny. The aggregated introgressions cover 94.6% of the C. briggsae genome, including 100% of the X chromosome. Surprisingly, we observed that two C. briggsae X fragments that produce C. nigoni male sterility as an introgression rescued hybrid F1 sterility in males fathered by C. briggsae, indicating that at least two separate X-autosome interactions are involved in the hybrid male sterility. In addition, we identified another two C. briggsae genomic intervals on the Chromosome II or IV, respectively, which can rescue the inviability, but not the sterility, of hybrid F1 males fathered by C. nigoni, suggesting the involvement of differential epistatic interactions in the asymmetric hybrid male fertility and inviability. Importantly, backcrossing of the rescued sterile males with C. nigoni led to isolation of a 1.1-Mb genomic interval that specifically interacts with an X-linked introgression, which is essential for hybrid male fertility. We further identified three C. briggsae genomic intervals on the Chromosome I, II and III, respectively that produce inviability in all F1 progeny dependent or independent of the parent-of-origin. Taken together, we identified multiple independent interacting loci that are responsible for asymmetric hybrid male and female sterility and inviability, which provides important insights into the asymmetric HI and lays a foundation for their molecular characterization.

scholarly journals Genome-wide misexpression associated with hybrid sterility in Mimulus (monkeyflower)

2019 ◽  
Author(s):  
Rachel E. Kerwin ◽  
Andrea L. Sweigart
Keyword(s):  
Male Sterility ◽  
Gene Expression Regulation ◽  
Hybrid Sterility ◽  
Introgression Line ◽  
Transcript Abundance ◽  
Stabilizing Selection ◽  
Hybrid Male ◽  
Specific Expression ◽  
Hybrid Male Sterility ◽  
Genome Wide

ABSTRACTDivergence in gene expression regulation is common between closely related species and may give rise to incompatibilities in their hybrid progeny. In this study, we investigated the relationship between regulatory evolution within species and reproductive isolation between species. We focused on a well-studied case of hybrid sterility between Mimulus guttatus and M. nasutus, two closely related yellow monkeyflower species, that is caused by two epistatic loci, hybrid male sterility 1 (hms1) and hybrid male sterility 2 (hms2). We quantified and compared global transcript abundance across male and female reproductive tissues (i.e. stamens and carpels) of M. guttatus and M. nasutus, as well as sterile and fertile progeny from an advanced M. nasutus-M. guttatus introgression line that carries the hms1-hms2 incompatibility. We observed substantial variation in transcript abundance between M. guttatus and M. nasutus, including distinct but overlapping patterns of tissue-biased expression, providing evidence for regulatory divergence between these species. Furthermore, we found pervasive genome-wide misexpression exclusively associated with hybrid sterility – only observed in the affected tissues (i.e. stamens) of sterile introgression hybrids. Examining patterns of allele-specific expression in sterile and fertile hybrids, we found evidence of cis- and trans- regulatory divergence, as well as cis-trans compensatory evolution (likely to be driven by stabilizing selection). However, regulatory divergence does not appear to cause misexpression in sterile hybrids, which instead likely manifests as a downstream consequence of sterility itself.

scholarly journals Hidden Effects of X Chromosome Introgressions on Spermatogenesis in Drosophila simulans × D. mauritiana Hybrids Unveiled by Interactions Among Minor Genetic Factors

Genetics ◽  
1998 ◽  
Vol 150 (2) ◽  
pp. 745-754 ◽  
Author(s):  
Xulio R Maside ◽  
José P Barral ◽  
Horacio F Naveira
Keyword(s):  
Male Sterility ◽  
X Chromosome ◽  
Seminal Vesicles ◽  
Genetic Dissection ◽  
Research Strategies ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Epistatic Interactions ◽  
Drosophila Mauritiana ◽  
Chromosome Segments

Abstract One of the most frequent outcomes of interspecific hybridizations in Drosophila is hybrid male sterility. Genetic dissection of this reproductive barrier has revealed that the number of responsible factors is very high and that these factors are frequently engaged in complex epistatic interactions. Traditionally, research strategies have been based on contrasting introgressions of chromosome segments that produce male sterility with those that allow fertility. Few studies have investigated the phenotypes associated with the boundary between fertility and sterility. In this study, we cointrogressed three different X chromosome segments from Drosophila mauritiana into D. simulans. Hybrid males with these three segments are usually fertile, by conventional fertility assays. However, their spermatogenesis shows a significant slowdown, most manifest at lower temperatures. Each of the three introgressed segments retards the arrival of sperm to the seminal vesicles. Other small disturbances in spermatogenesis are evident, which altogether lead to an overall reduction in the amount of motile sperm in their seminal vesicles. These results suggest that a delay in the timing of spermatogenesis, which might be brought about by the cumulative action of many different factors of minor segment, may be the primary cause of hybrid male sterility.

Genetic Complexity Underlying Hybrid Male Sterility in Drosophila

Genetics ◽  
2004 ◽  
Vol 166 (2) ◽  
pp. 789-796 ◽  
Author(s):  
Kyoichi Sawamura ◽  
John Roote ◽  
Chung-I Wu ◽  
Masa-Toshi Yamamoto
Keyword(s):  
Male Sterility ◽  
Related Species ◽  
Synergistic Effects ◽  
Female Sterility ◽  
Hybrid Male ◽  
Hybrid Female ◽  
Hybrid Male Sterility ◽  
Closely Related Species ◽  
Genetic Complexity ◽  
Recessive Genes

Abstract Recent genetic analyses of closely related species of Drosophila have indicated that hybrid male sterility is the consequence of highly complex synergistic effects among multiple genes, both conspecific and heterospecific. On the contrary, much evidence suggests the presence of major genes causing hybrid female sterility and inviability in the less-related species, D. melanogaster and D. simulans. Does this contrast reflect the genetic distance between species? Or, generally, is the genetic basis of hybrid male sterility more complex than that of hybrid female sterility and inviability? To clarify this point, the D. simulans introgression of the cytological region 34D-36A to the D. melanogaster genome, which causes recessive male sterility, was dissected by recombination, deficiency, and complementation mapping. The 450-kb region between two genes, Suppressor of Hairless and snail, exhibited a strong effect on the sterility. Males are (semi-)sterile if this region of the introgression is made homozygous or hemizygous. But no genes in the region singly cause the sterility; this region has at least two genes, which in combination result in male sterility. Further, the males are less fertile when heterozygous with a larger introgression, which suggests that dominant modifiers enhance the effects of recessive genes of male sterility. Such an epistatic view, even in the less-related species, suggests that the genetic complexity is special to hybrid male sterility.

scholarly journals Age and genetic background modify hybrid male sterility in house mice

2020 ◽  
Author(s):  
Samuel J. Widmayer ◽  
Mary Ann Handel ◽  
David L. Aylor
Keyword(s):  
Male Sterility ◽  
House Mouse ◽  
Genetic Background ◽  
Genetic Architecture ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
X Chromosomes ◽  
Age Related ◽  
Age Dependent ◽  
In The Wild

AbstractHybrid male sterility (HMS) contributes to reproductive isolation commonly observed among house mouse (Mus musculus) subspecies, both in the wild and in laboratory crosses. Incompatibilities involving specific Prdm9 alleles and certain Chromosome (Chr) X genotypes are known determinants of fertility and HMS, and previous work in the field has demonstrated that genetic background modifies these two major loci. We constructed hybrids that have identical genotypes at Prdm9 and identical X chromosomes, but differ widely across the rest of the genome. In each case, we crossed female PWK/PhJ mice representative of the M. m. musculus subspecies to males from a classical inbred strain representative of M. m. domesticus: 129S1/SvImJ, A/J, C57BL/6J, or DBA/2J. We detected three distinct trajectories of fertility among the hybrids using breeding experiments. The PWK129S1 males were always infertile. PWKDBA2 males were fertile, despite their genotypes at the major HMS loci. We also observed age-dependent changes in fertility parameters across multiple genetic backgrounds. The PWKB6 and PWKAJ males were always infertile before 15 weeks and after 35 weeks, yet some PWKB6 and PWKAJ males were fertile between fifteen and 35 weeks. This observation could resolve previous contradictory reports about the fertility of PWKB6. Taken together, these results point to multiple segregating HMS modifier alleles, some of which have age-related modes of action. The ultimate identification of these alleles and their age-related mechanisms will advance understanding both of the genetic architecture of HMS and of how reproductive barriers are maintained between house mouse subspecies.

scholarly journals Specific Interactions Between Autosome and X Chromosomes Cause Hybrid Male Sterility in Caenorhabditis Species

Genetics ◽  
2019 ◽  
Vol 212 (3) ◽  
pp. 801-813 ◽  
Author(s):  
Yu Bi ◽  
Xiaoliang Ren ◽  
Runsheng Li ◽  
Qiutao Ding ◽  
Dongying Xie ◽  
...  
Keyword(s):  
Male Sterility ◽  
Male Fertility ◽  
Genetic Mechanism ◽  
Hybrid Male ◽  
Female Progeny ◽  
Hybrid Male Sterility ◽  
Male And Female ◽  
F1 Progeny ◽  
Asymmetric Hybrid ◽  
Parent Of Origin

Hybrid male progeny from interspecies crosses are more prone to sterility or inviability than hybrid female progeny, and the male sterility and inviability often demonstrate parent-of-origin asymmetry. However, the underlying genetic mechanism of asymmetric sterility or inviability remains elusive. We previously established a genome-wide hybrid incompatibility (HI) landscape between Caenorhabditis briggsae and C. nigoni by phenotyping a large collection of C. nigoni strains each carrying a C. briggsae introgression. In this study, we systematically dissect the genetic mechanism of asymmetric sterility and inviability in both hybrid male and female progeny between the two species. Specifically, we performed reciprocal crosses between C. briggsae and different C. nigoni strains that each carry a GFP-labeled C. briggsae genomic fragment referred to as introgression, and scored the HI phenotypes in the F1 progeny. The aggregated introgressions cover 94.6% of the C. briggsae genome, including 100% of the X chromosome. Surprisingly, we observed that two C. briggsaeX fragments that produce C. nigoni male sterility as an introgression rescued hybrid F1 sterility in males fathered by C. briggsae. Subsequent backcrossing analyses indicated that a specific interaction between the X-linked interaction and one autosome introgression is required to rescue the hybrid male sterility. In addition, we identified another two C. briggsae genomic intervals on chromosomes II and IV that can rescue the inviability, but not the sterility, of hybrid F1 males fathered by C. nigoni, suggesting the involvement of differential epistatic interactions in the asymmetric hybrid male fertility and inviability. Importantly, backcrossing of the rescued sterile males with C. nigoni led to the isolation of a 1.1-Mb genomic interval that specifically interacts with an X-linked introgression, which is essential for hybrid male fertility. We further identified three C. briggsae genomic intervals on chromosome I, II, and III that produced inviability in all F1 progeny, dependent on or independent of the parent-of-origin. Taken together, we identified multiple independent interacting loci that are responsible for asymmetric hybrid male and female sterility, and inviability, which lays a foundation for their molecular characterization.

scholarly journals Promoter hypermethylation of PIWI/piRNA pathway genes associated with diminished pachytene piRNA production in bovine hybrid male sterility

Epigenetics ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. 914-931 ◽  
Author(s):  
Gong-Wei Zhang ◽  
Ling Wang ◽  
Huiyou Chen ◽  
Jiuqiang Guan ◽  
Yuhui Wu ◽  
...  
Keyword(s):  
Male Sterility ◽  
Promoter Hypermethylation ◽  
Hybrid Male ◽  
Hybrid Male Sterility ◽  
Pirna Pathway
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