scholarly journals Warmer temperature accelerates methane emissions from the Zoige wetland on the Tibetan Plateau without changing methanogenic community composition

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Mengmeng Cui ◽  
Anzhou Ma ◽  
Hongyan Qi ◽  
Xuliang Zhuang ◽  
Guoqiang Zhuang ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Community Composition ◽  
Methane Emissions ◽  
The Tibetan Plateau ◽  
Methanogenic Community ◽  
Zoige Wetland

scholarly journals Methane emissions from an alpine wetland on the Tibetan Plateau: Neglected but vital contribution of the nongrowing season

2015 ◽  
Vol 120 (8) ◽  
pp. 1475-1490 ◽  
Author(s):  
Weimin Song ◽  
Hao Wang ◽  
Guangshuai Wang ◽  
Litong Chen ◽  
Zhenong Jin ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Methane Emissions ◽  
The Tibetan Plateau ◽  
Alpine Wetland

scholarly journals Methanogenesis from Methanol at Low Temperatures by a Novel Psychrophilic Methanogen, “Methanolobus psychrophilus” sp. nov., Prevalent in Zoige Wetland of the Tibetan Plateau

2008 ◽  
Vol 74 (19) ◽  
pp. 6114-6120 ◽  
Author(s):  
Guishan Zhang ◽  
Na Jiang ◽  
Xiaoli Liu ◽  
Xiuzhu Dong
Keyword(s):  
Tibetan Plateau ◽  
Methane Emission ◽  
Low Temperatures ◽  
Methanol Conversion ◽  
Wetland Soil ◽  
The Tibetan Plateau ◽  
Hydrogenotrophic Methanogenesis ◽  
Efficient Type ◽  
Zoige Wetland ◽  
A New Species

ABSTRACT The Zoige wetland of the Tibetan plateau is at permanent low temperatures and is a methane emission heartland of the plateau; however, cold-adaptive methanogens in the soil are poorly understood. In this study, a variety of methanogenic enrichments at 15�C and 30�C were obtained from the wetland soil. It was demonstrated that hydrogenotrophic methanogenesis was the most efficient type at 30�C, while methanol supported the highest methanogenesis rate at 15�C. Moreover, methanol was the only substrate to produce methane more efficiently at 15�C than at 30�C. A novel psychrophilic methanogen, strain R15, was isolated from the methanol enrichment at 15�C. Phylogenetic analysis placed strain R15 within the genus Methanolobus, loosely clustered with Methanolobus taylorii (96.7% 16S rRNA similarity). R15 produced methane from methanol, trimethylamine, and methyl sulfide and differed from other Methanolobus species by growing and producing methane optimally at 18�C (specific growth rate of 0.063 � 0.001 h−1) and even at 0�C. Based on these characteristics, R15 was proposed to be a new species and named “Methanolobus psychrophilus” sp. nov. The Km and V max of R15 for methanol conversion were determined to be 87.5 � 0.4 μM and 0.39 � 0.04 mM h−1 at 18�C, respectively, indicating a high affinity and conversion efficiency for methanol. The proportion of R15 in the soil was determined by quantitative PCR, and it accounted for 17.2% � 2.1% of the total archaea, enumerated as 107 per gram of soil; the proportion was increased to 42.4% � 2.3% in the methanol enrichment at 15�C. This study suggests that the psychrophilic methanogens in the Zoige wetland are likely to be methylotrophic and to play a role in methane emission of the wetland.

scholarly journals Temperature and Precipitation Drive Elevational Patterns of Microbial Beta Diversity in Alpine Grasslands

2021 ◽  
Author(s):  
Xiaoqin Yang ◽  
Yue Li ◽  
Bin Niu ◽  
Qiuyu Chen ◽  
Yilun Hu ◽  
...  
Keyword(s):  
Species Richness ◽  
Tibetan Plateau ◽  
Community Composition ◽  
Beta Diversity ◽  
Microbial Community Composition ◽  
Mean Annual Precipitation ◽  
Mean Annual Temperature ◽  
The Tibetan Plateau ◽  
Alpine Grasslands ◽  
Temperature And Precipitation

Abstract Understanding the mechanisms underlying biodiversity patterns is a central issue in ecology, while how temperature and precipitation jointly control the elevational patterns of microbes is understudied. Here, we studied the effects of temperature, precipitation and their interactions on the alpha and beta diversity of soil archaea and bacteria in alpine grasslands along an elevational gradient of 4,300-5,200 m on the Tibetan Plateau. Alpha diversity was examined on the basis of species richness and evenness, and beta diversity was quantified with the recently developed metric of local contributions to beta diversity (LCBD). Typical alpine steppe and meadow ecosystems were distributed below and above 4,850 m, respectively, which was consistent with the two main constraints of mean annual temperature (MAT) and mean annual precipitation (MAP). Species richness and evenness showed decreasing elevational patterns in archaea and nonsignificant or U-shaped patterns in bacteria. The LCBD of both groups exhibited significant U-shaped elevational patterns, with the lowest values occurring at 4,800 m. For the three diversity metrics, soil pH was the primary explanatory variable in archaea, explaining over 20.1% of the observed variation, whereas vegetation richness, total nitrogen and the K/Al ratio presented the strongest effects on bacteria, with relative importance values of 16.1%, 12.5% and 11.6%, respectively. For the microbial community composition of both archaea and bacteria, the moisture index showed the dominant effect, explaining 17.6% of the observed variation, followed by MAT and MAP. Taken together, temperature and precipitation exerted considerable indirect effects on microbial richness and evenness through local environmental and energy supply-related variables, such as vegetation richness, whereas temperature exerted a larger direct influence on LCBD and the community composition. Our findings highlighted the profound influence of temperature and precipitation interactions on microbial beta diversity in alpine grasslands on the Tibetan Plateau.

Analyzing the nitrate reductase gene (nirK) community in the peat soil of the Zoige Wetland of the Tibetan Plateau

2017 ◽  
Vol 37 (19) ◽  
Author(s):  
王蓥燕 WANG Yingyan ◽  
卢圣鄂 LU Sheng'e ◽  
陈小敏 CHEN Xiaomin ◽  
李跃飞 LI Yuefei ◽  
辜运富 GU Yunfu
Keyword(s):  
Tibetan Plateau ◽  
Nitrate Reductase ◽  
Peat Soil ◽  
The Tibetan Plateau ◽  
Nitrate Reductase Gene ◽  
Reductase Gene ◽  
Zoige Wetland

scholarly journals Prokaryotic Community Structure Driven by Salinity and Ionic Concentrations in Plateau Lakes of the Tibetan Plateau

2016 ◽  
Vol 82 (6) ◽  
pp. 1846-1858 ◽  
Author(s):  
Zhi-Ping Zhong ◽  
Ying Liu ◽  
Li-Li Miao ◽  
Fang Wang ◽  
Li-Min Chu ◽  
...  
Keyword(s):  
Community Structure ◽  
Tibetan Plateau ◽  
Community Composition ◽  
Qaidam Basin ◽  
Nitrogen Concentration ◽  
Distribution Patterns ◽  
The Tibetan Plateau ◽  
Prokaryotic Community ◽  
Content Type ◽  
Ionic Concentrations

ABSTRACTThe prokaryotic community composition and diversity and the distribution patterns at various taxonomic levels across gradients of salinity and physiochemical properties in the surface waters of seven plateau lakes in the Qaidam Basin, Tibetan Plateau, were evaluated using Illumina MiSeq sequencing. These lakes included Lakes Keluke (salinity, <1 g/liter), Qing (salinity, 5.5 to 6.6 g/liter), Tuosu (salinity, 24 to 35 g/liter), Dasugan (salinity, 30 to 33 g/liter), Gahai (salinity, 92 to 96 g/liter), Xiaochaidan (salinity, 94 to 99 g/liter), and Gasikule (salinity, 317 to 344 g/liter). The communities were dominated byBacteriain lakes with salinities of <100 g/liter and byArchaeain Lake Gasikule. The clades At12OctB3 andSalinibacter, previously reported only in hypersaline environments, were found in a hyposaline lake (salinity, 5.5 to 6.6 g/liter) at an abundance of ∼1.0%, indicating their ecological plasticity. Salinity and the concentrations of the chemical ions whose concentrations covary with salinity (Mg2+, K+, Cl−, Na+, SO42−, and Ca2+) were found to be the primary environmental factors that directly or indirectly determined the composition and diversity at the level of individual clades as well as entire prokaryotic communities. The distribution patterns of two phyla, five classes, five orders, five families, and three genera were well predicted by salinity. The variation of the prokaryotic community structure also significantly correlated with the dissolved oxygen concentration, pH, the total nitrogen concentration, and the PO43−concentration. Such correlations varied depending on the taxonomic level, demonstrating the importance of comprehensive correlation analyses at various taxonomic levels in evaluating the effects of environmental variable factors on prokaryotic community structures. Our findings clarify the distribution patterns of the prokaryotic community composition in plateau lakes at the levels of individual clades as well as whole communities along gradients of salinity and ionic concentrations.

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