scholarly journals Single-Molecule Imaging Reveals Dynamics of CREB Transcription Factor Bound to Its Target Sequence

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Noriyuki Sugo ◽  
Masatoshi Morimatsu ◽  
Yoshiyuki Arai ◽  
Yoshinori Kousoku ◽  
Aya Ohkuni ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Target Sequence ◽  
Single Molecule Imaging ◽  
Creb Transcription Factor

scholarly journals Single-molecule imaging of CREB transcription factor in human cortical-like neurons

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S216-S217
Author(s):  
Yuri Atsumi ◽  
Noriyuki Sugo ◽  
Ryohei Iwata ◽  
Pierre Vanderhaeghen ◽  
Nobuhiko Yamamoto
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Creb Transcription Factor

scholarly journals Single-molecule imaging reveals the interplay between transcription factors, nucleosomes, and transcriptional bursting

2018 ◽  
Author(s):  
Benjamin T. Donovan ◽  
Anh Huynh ◽  
David A. Ball ◽  
Michael G. Poirier ◽  
Daniel R. Larson ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Single Molecule ◽  
Target Genes ◽  
Burst Size ◽  
Yeast Cells ◽  
Single Molecule Imaging ◽  
Transcriptional Bursting

SummaryTranscription factors show rapid and reversible binding to chromatin in living cells, and transcription occurs in sporadic bursts, but how these phenomena are related is unknown. Using a combination of in vitro and in vivo single-molecule imaging approaches, we directly correlated binding of the transcription factor Gal4 with the transcriptional bursting kinetics of the Gal4 target genes GAL3 and GAL10 in living yeast cells. We find that Gal4 dwell times sets the transcriptional burst size. Gal4 dwell time depends on the affinity of the binding site and is reduced by orders of magnitude by nucleosomes. Using a novel imaging platform, we simultaneously tracked transcription factor binding and transcription at one locus, revealing the timing and correlation between Gal4 binding and transcription. Collectively, our data support a model where multiple polymerases initiate during a burst as long as the transcription factor is bound to DNA, and a burst terminates upon transcription factor dissociation.

scholarly journals Single Molecule Imaging of Transcription Factor-DNA Interactions in Zebrafish Development

2017 ◽  
Vol 112 (3) ◽  
pp. 152a
Author(s):  
Matthias Reisser ◽  
Shai R. Joseph ◽  
Nadine L. Vastenhouw ◽  
J. Christof M. Gebhardt
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Dna Interactions ◽  
Zebrafish Development

scholarly journals Single-molecule imaging of transcription factor binding to DNA in live mammalian cells

2013 ◽  
Vol 10 (5) ◽  
pp. 421-426 ◽  
Author(s):  
J Christof M Gebhardt ◽  
David M Suter ◽  
Rahul Roy ◽  
Ziqing W Zhao ◽  
Alec R Chapman ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Mammalian Cells ◽  
Transcription Factor Binding ◽  
Single Molecule Imaging ◽  
Factor Binding
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