scholarly journals Propagation of pure fetal and maternal mesenchymal stromal cells from terminal chorionic villi of human term placenta

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Smitha Mathews ◽  
K. Lakshmi Rao ◽  
K. Suma Prasad ◽  
M. K. Kanakavalli ◽  
A. Govardhana Reddy ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Term Placenta ◽  
Chorionic Villi ◽  
Term Placenta

Human Term Placenta-Derived Mesenchymal Stromal Cells Are Less Prone to Osteogenic Differentiation Than Bone Marrow-Derived Mesenchymal Stromal Cells

2011 ◽  
Vol 20 (4) ◽  
pp. 635-646 ◽  
Author(s):  
Gregor A. Pilz ◽  
Christine Ulrich ◽  
Manuel Ruh ◽  
Harald Abele ◽  
Richard Schäfer ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Term Placenta ◽  
Term Placenta

Phenotypic and Functional Characterization of Mesenchymal Stem/Multipotent Stromal Cells From Decidua Parietalis of Human Term Placenta

2016 ◽  
Vol 23 (9) ◽  
pp. 1193-1207 ◽  
Author(s):  
Mohamed H. Abumaree ◽  
F. M. Abomaray ◽  
N. A. Alshehri ◽  
A. Almutairi ◽  
A. S. AlAskar ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Functional Characterization ◽  
Multipotent Stromal Cells ◽  
Human Term Placenta ◽  
Term Placenta

Potential of 5-azacytidine induction decidual stromal cells from maternal human term placenta towards cardiomyocyte-like cells in serum-free medium

2015 ◽  
Vol 16 (3) ◽  
pp. 477-485 ◽  
Author(s):  
Gecai Chen ◽  
Aihuan Yue ◽  
Zhongbao Ruan ◽  
Yigang Yin ◽  
Ruzhu Wang ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Human Term Placenta ◽  
Serum Free ◽  
Term Placenta

Morphological Features of Mesenhymal Stroma Cells of Chorionic Villi

2017 ◽  
Vol 72 (1) ◽  
pp. 76-83 ◽  
Author(s):  
N. V. Nizyaeva ◽  
Т. V. Sukhacheva ◽  
G. V. Kulikova ◽  
M. N. Nagovitsyna ◽  
N. E. Kan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Placental Tissue ◽  
Control Group ◽  
Chorionic Villi ◽  
Mean Values ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

Background: Nowadays autologous mesenchymal placental stromal cells (MSCs) may use to treat for various diseases both of the mother and the child. Stroma of the placenta villi is appropriated origin for cell culture isolation. Aim of the study was to evaluate the possibility for selection and use of placental tissue for mesenchymal stromal cells. Materials and methods: The present study was based on 45 placental samples of women aged 27−38 yy. who underwent surgical delivery at 36−40 weeks of gestation. 30 of these women have been enrolled in the basic group including children with congenital abnormalities (CA). The comparison group consisted of 15 patients with physiological pregnancy. We performed histological examination (with hematoxylin and eosin staining), immunohistochemical examination (with use monoclonal antibodies CD90 (1:25; Abcam, UK), СD105 (1:500; Abcam, UK), CD44 (1:25; Dako), СD73 (1:200, Abcam, UK), and electron microscopy (by microscope Philips/FEI Corporation, Eindhoven, Holland). Eclipse 80i microscope (Nikon Corporation, Japan) was used to examine the immunohistochemical reactions as a brown staining. The evaluation of the intensity of reaction was conducted by NIS-Elements Advanced Research 3.2 program (Czech Republic). Student’s t-test and analysis of variance were used to compare the mean values. Differences were considered statistically significant at p0.05. Results: Interstitial cells of the stroma of the villi with CA had fibroblastic differentiation as revealed degenerative changes of the cells. The histologic examination with hematoxylin and eosin staining revealed significant fibrosis of the stroma of the placenta villi in CA group (p0,01). Immunohistochemical study of stem and intermediate chorionic villi revealed no significant differences in staining of CD44+, СD90+, СD73+, and CD105+ cells if compared to the control group (p0.05). Although CD105 expression was significantly lower in the CA group (0.058±0.0049) than in the control group (0.088±0.0039) (p0.05). However, electron microscopy detected the villi interstitial stromal cells with fibroblastic differentiation in CA group. Conclusions: Thus, it is necessary to exclude placenta with obstetrical history, somatic, and congenital pathology of the mother and the child when selecting the placental cell culture. Moreover, choosing a sample the morphological structure of the placenta should be taken into consideration. However, congenital malformations of the fetus, pathology of the mother cultivate mesenchymal stromal cells of placentas is inappropriate and should be taken advantage of the donor cells.

scholarly journals Bone marrow-derived mesenchymal stromal cells differ in their attachment to fibronectin-derived peptides from term placenta-derived mesenchymal stromal cells

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Jan K. Maerz ◽  
Lorenzo P. Roncoroni ◽  
David Goldeck ◽  
Tanja Abruzzese ◽  
Hubert Kalbacher ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Term Placenta
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