scholarly journals Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Taku Tanaka ◽  
Tadashi Yokoi ◽  
Fuminobu Tamalu ◽  
Shu-Ichi Watanabe ◽  
Sachiko Nishina ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Retinal Ganglion Cells ◽  
Pluripotent Stem Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Induced Pluripotent

scholarly journals Modeling Glaucoma: Retinal Ganglion Cells Generated from Induced Pluripotent Stem Cells of Patients with SIX6 Risk Allele Show Developmental Abnormalities

Stem Cells ◽  
2017 ◽  
Vol 35 (11) ◽  
pp. 2239-2252 ◽  
Author(s):  
Pooja Teotia ◽  
Matthew J. Van Hook ◽  
Christopher S. Wichman ◽  
R. Rand Allingham ◽  
Michael A. Hauser ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Retinal Ganglion Cells ◽  
Pluripotent Stem Cells ◽  
Risk Allele ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Developmental Abnormalities ◽  
Induced Pluripotent

scholarly journals Capacity of Retinal Ganglion Cells Derived from Human Induced Pluripotent Stem Cells to Suppress T-Cells

2020 ◽  
Vol 21 (21) ◽  
pp. 7831
Author(s):  
Ayaka Edo ◽  
Sunao Sugita ◽  
Yoko Futatsugi ◽  
Junki Sho ◽  
Akishi Onishi ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cells ◽  
Induced Pluripotent Stem Cells ◽  
Retinal Ganglion Cells ◽  
Pluripotent Stem Cells ◽  
Transforming Growth Factor ◽  
Ganglion Cells ◽  
Critical Role ◽  
Retinal Ganglion ◽  
Induced Pluripotent

Retinal ganglion cells (RGCs) are impaired in patients such as those with glaucoma and optic neuritis, resulting in permanent vision loss. To restore visual function, development of RGC transplantation therapy is now underway. Induced pluripotent stem cells (iPSCs) are an important source of RGCs for human allogeneic transplantation. We therefore analyzed the immunological characteristics of iPSC-derived RGCs (iPSC-RGCs) to evaluate the possibility of rejection after RGC transplantation. We first assessed the expression of human leukocyte antigen (HLA) molecules on iPSC-RGCs using immunostaining, and then evaluated the effects of iPSC-RGCs to activate lymphocytes using the mixed lymphocyte reaction (MLR) and iPSC-RGC co-cultures. We observed low expression of HLA class I and no expression of HLA class II molecules on iPSC-RGCs. We also found that iPSC-RGCs strongly suppressed various inflammatory immune cells including activated T-cells in the MLR assay and that transforming growth factor-β2 produced by iPSC-RGCs played a critical role in suppression of inflammatory cells in vitro. Our data suggest that iPSC-RGCs have low immunogenicity, and immunosuppressive capacity on lymphocytes. Our study will contribute to predicting immune attacks after RGC transplantation.

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