scholarly journals Effects of Tet-mediated Oxidation Products of 5-Methylcytosine on DNA Transcription in vitro and in Mammalian Cells

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Changjun You ◽  
Debin Ji ◽  
Xiaoxia Dai ◽  
Yinsheng Wang
Keyword(s):  
Mammalian Cells ◽  
Oxidation Products ◽  
Dna Transcription ◽  
Transcription In Vitro ◽  
Mediated Oxidation

Synthetic octapeptide pyroGLU-ASP-ASP-SER-ASP-GLU-GLU-ASN controls DNA transcription in vitro by RNA polymerase II

1993 ◽  
Vol 49 (10) ◽  
pp. 902-905 ◽  
Author(s):  
A. Angiolillo ◽  
A. Desgro ◽  
V. Marsili ◽  
F. Panara ◽  
G. L. Gianfranceschi
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Dna Transcription ◽  
Transcription In Vitro

To the Mechanism of Horseradish Peroxidase-Mediated Degradation of a Recalcitrant Dye Remazol Brilliant Blue R

2001 ◽  
Vol 66 (4) ◽  
pp. 663-675 ◽  
Author(s):  
Markéta Mikšanová ◽  
Jiří Hudeček ◽  
Jan Páca ◽  
Marie Stiborová
Keyword(s):  
Molecular Weight ◽  
Horseradish Peroxidase ◽  
Oxidation Products ◽  
Brilliant Blue ◽  
Radical Mechanism ◽  
Remazol Brilliant Blue R ◽  
Radical Trapping ◽  
Optimum Ph ◽  
Mediated Oxidation

Thein vitroenzymatic metabolism of a recalcitrant dye Remazol Brilliant Blue R (RBBR) was investigated using horseradish peroxidase (HRP). At optimum pH (4.5), the apparent Michaelis constant (KM) value for the oxidation of RBBR catalyzed by HRP is 14.8 μmol l-1. HRP-mediated conversion of RBBR proceedsviaa conventional peroxidase reaction, by a sequential one-electron oxidation of two molecules of RBBR by the peroxidase Compounds I and II. The oxidation is inhibited by radical trapping agents (nicotinamide adenine dinucleotide reduced (NADH), ascorbate, glutathione). This confirms that the peroxidase-mediated oxidation of RBBR proceedsviaradical mechanism. Gel permeation profile of the RBBR oxidation products shows that the pattern of molecular weight distribution was shifted to the higher molecular weight region indicating formation of RBBR oligomers. In addition to HRP, the RBBR dye is also oxidized by another peroxidase, the mammalian lactoperoxidase.

Amplification of 4‘-ThioDNA in the Presence of 4‘-Thio-dTTP and 4‘-Thio-dCTP, and 4‘-ThioDNA-Directed Transcription in Vitro and in Mammalian Cells

2007 ◽  
Vol 129 (50) ◽  
pp. 15424-15425 ◽  
Author(s):  
Naonori Inoue ◽  
Aki Shionoya ◽  
Noriaki Minakawa ◽  
Akiko Kawakami ◽  
Naoki Ogawa ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Transcription In Vitro

Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

1972 ◽  
Vol 30 ◽  
pp. 350-351
Author(s):  
K. Shankar Narayan ◽  
Kailash C. Gupta ◽  
Tohru Okigaki
Keyword(s):  
Ultraviolet Light ◽  
Mammalian Cells ◽  
Biological Effects ◽  
Survival Rates ◽  
Chinese Hamster ◽  
Cell Types ◽  
Differential Sensitivity ◽  
Ultrastructural Changes ◽  
Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

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