scholarly journals Genomic and transcriptome analyses reveal that MAPK- and phosphatidylinositol-signaling pathways mediate tolerance to 5-hydroxymethyl-2-furaldehyde for industrial yeast Saccharomyces cerevisiae

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Qian Zhou ◽  
Z. Lewis Liu ◽  
Kang Ning ◽  
Anhui Wang ◽  
Xiaowei Zeng ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Signaling Pathways ◽  
Industrial Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Transcriptome Analyses

scholarly journals Comparative Proteomics Analysis Reveals Unique Early Signaling Response of Saccharomyces cerevisiae to Oxidants with Different Mechanism of Action

2020 ◽  
Vol 22 (1) ◽  
pp. 167
Author(s):  
Prajita Pandey ◽  
Khadiza Zaman ◽  
Laszlo Prokai ◽  
Vladimir Shulaev
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Signaling Pathways ◽  
Mechanism Of Action ◽  
Mapk Signaling ◽  
Comparative Proteomics ◽  
Mass Analyzer ◽  
Label Free ◽  
Proteomics Analysis ◽  
Yeast Saccharomyces Cerevisiae ◽  
Signaling Mechanism

The early signaling events involved in oxidant recognition and triggering of oxidant-specific defense mechanisms to counteract oxidative stress still remain largely elusive. Our discovery driven comparative proteomics analysis revealed unique early signaling response of the yeast Saccharomyces cerevisiae on the proteome level to oxidants with a different mechanism of action as early as 3 min after treatment with four oxidants, namely H2O2, cumene hydroperoxide (CHP), and menadione and diamide, when protein abundances were compared using label-free quantification relying on a high-resolution mass analyzer (Orbitrap). We identified significant regulation of 196 proteins in response to H2O2, 569 proteins in response to CHP, 369 proteins in response to menadione and 207 proteins in response to diamide. Only 17 proteins were common across all treatments, but several more proteins were shared between two or three oxidants. Pathway analyses revealed that each oxidant triggered a unique signaling mechanism associated with cell survival and repair. Signaling pathways mostly regulated by oxidants were Ran, TOR, Rho, and eIF2. Furthermore, each oxidant regulated these pathways in a unique way indicating specificity of response to oxidants having different modes of action. We hypothesize that interplay of these signaling pathways may be important in recognizing different oxidants to trigger different downstream MAPK signaling cascades and to induce specific responses.

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