scholarly journals Transcriptional regulation of adrenomedullin by oncostatin M in human astroglioma cells: Implications for tumor invasion and migration

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Seul Ye Lim ◽  
So-Hee Ahn ◽  
Hyunju Park ◽  
Jungsul Lee ◽  
Kyungsun Choi ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Tumor Invasion ◽  
Oncostatin M ◽  
Invasion And Migration ◽  
And Migration ◽  
Astroglioma Cells

Six‐transmembrane epithelial antigen of the prostate 1 is associated with tumor invasion and migration in endometrial carcinomas

2019 ◽  
Vol 120 (7) ◽  
pp. 11172-11189 ◽  
Author(s):  
Jiali Sun ◽  
Guoxin Ji ◽  
Jie Xie ◽  
Zhi Jiao ◽  
Haozheng Zhang ◽  
...  
Keyword(s):  
Tumor Invasion ◽  
Invasion And Migration ◽  
And Migration ◽  
Endometrial Carcinomas

scholarly journals miR-720 inhibits tumor invasion and migration in breast cancer by targeting TWIST1

2013 ◽  
Vol 35 (2) ◽  
pp. 469-478 ◽  
Author(s):  
Lin-Zi Li ◽  
Chris Zhiyi Zhang ◽  
Li-Li Liu ◽  
Chun Yi ◽  
Shi-Xun Lu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Invasion ◽  
Invasion And Migration ◽  
And Migration

scholarly journals circEPS15 Overexpression in Hepatocellular Carcinoma Modulates Tumor Invasion and Migration

2020 ◽  
Author(s):  
Maolin Tian ◽  
Gang Li ◽  
Bin Jiang ◽  
Sadula Abuduhaibaier ◽  
Dianrong Xiu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Cycle ◽  
Tumor Invasion ◽  
Expression Profiles ◽  
The Cancer Genome Atlas ◽  
Circular Rnas ◽  
Invasion And Migration ◽  
Qrt Pcr ◽  
Cerna Network ◽  
And Migration

Abstract Background: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. Recent evidence indicates that circular RNAs (circRNAs) play important roles in tissue development, gene regulation, and carcinogenesis. However, whether circRNAs are involved in HCC progression and encode functional proteins remains largely unknown.Methods: Circular RNA microarrays were performed using three pathologically diagnosed HCC samples and their paired adjacent normal liver tissues. Cell invasion, migration, cell cycle, and apoptosis after circRNA overexpression were measured using a transwell culture system, a wound healing assay, and flow cytometry . Full-length, mutated, and truncated sequences of circEPS15 with a FLAG tag were inserted inside a circular expression vector. Western blotting was used to confirm circEPS15 expression and the requirement of internal ribosomal entry site (IRES) elements within the circRNA. The miRNA and mRNA expression profiles were obtained by analyzing data retrieved from The Cancer Genome Atlas (TCGA) database. We then constructed a ceRNA network of mRNAs, miRNAs, and circEPS15. Using tissue samples from own patients, we also verified certain analytical results with quantitative real-time PCR (qRT-PCR).Results: The expression of circEPS15 was downregulated in HCC tissues, and the survival curves showed that low circEPS15 levels were associated with poor overall survival in HCC patients. Overexpression of circEPS15 suppressed tumor invasion and migration by inhibiting the TJP1/CDH2/VIM signaling pathway and retarded cell cycle progression, but it had no effect on cell apoptosis. ceRNA analysis and qRT-PCR showed that there might be a circRNA (circEPS15)-miRNA (miR-24-3p)-mRNA (CIDEA) network in HCC. The spanning junction open reading frame in circEPS15 driven by IRES encoded a novel protein.Conclusions: Endogenous circEPS15 plays a novel role in repressing HCC through the ceRNA network and encoding a functional protein.

scholarly journals circEPS15 Overexpression in Hepatocellular Carcinoma Modulates Tumor Invasion and Migration

2021 ◽  
Author(s):  
Maolin Tian ◽  
Gang Li ◽  
Bin Jiang ◽  
Abuduhaibaier Sadula ◽  
Dianrong Xiu ◽  
...  
Keyword(s):  
Tumor Invasion ◽  
Expression Profiles ◽  
The Cancer Genome Atlas ◽  
Circular Rnas ◽  
Functional Protein ◽  
Entry Site ◽  
Poor Overall Survival ◽  
Invasion And Migration ◽  
Cerna Network ◽  
And Migration

Abstract BackgroundHepatocellular carcinoma (HCC) is one kind of the leading causes of cancer-related deaths in the world. Recent evidence indicates that circular RNAs (circRNAs) play important roles in tissue development, gene transcription, signal regulation and tumorigenesis. However, whether circRNAs are involved in HCC progression and encode functional proteins remains largely unknown.MethodscircRNAs microarrays wereperformed using three pathologically diagnosed HCC samples and their paired adjacent normal liver tissues. Cell invasion, migration, cycle, and apoptosis post circRNA overexpression were measured using a transwell culture system, a wound healing assay, and flow cytometry. Full-length, mutated, and truncated sequences of circEPS15 with a FLAG tag were inserted into a circular expression vector. Western blotting was used to confirm circEPS15 expression and the requirement of internal ribosomal entry site (IRES) elements within the circRNA. The miRNA and mRNA expression profiles were obtained by analyzing data retrieved from The Cancer Genome Atlas (TCGA) database. We then constructed a ceRNA network ofcircEPS15, miRNAs, and mRNAs. ResultsThe expression of circEPS15 was downregulated in HCC tissues, and the survival curves showed that low circEPS15 levels were associated with poor overall survival in HCC patients. Overexpression of circEPS15 suppressed tumor invasion and migration by inhibiting the TJP1/CDH2/VIM signaling pathway and retarded cell cycle progression, yet had no effect on cell apoptosis. ceRNA analysis and qRT-PCR results suggest a possible circRNA (circEPS15)-miRNA (miR-24-3p)-mRNA (CIDEA) network in HCC. The spanning junction open reading frame in circEPS15 driven by IRES encoded a novel protein.ConclusionsEndogenous circEPS15 plays a novel role in repressing HCC through the ceRNA network and encoding a functional protein.

Non-small cell lung cancer: miR-30d suppresses tumor invasion and migration by directly targeting NFIB

2017 ◽  
Vol 39 (12) ◽  
pp. 1827-1834 ◽  
Author(s):  
Yubing Wu ◽  
Jingnan Zhang ◽  
Shizhen Hou ◽  
Ziming Cheng ◽  
Maoxi Yuan
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Tumor Invasion ◽  
Small Cell ◽  
Small Cell Lung ◽  
Invasion And Migration ◽  
And Migration

GALNT14 mediates tumor invasion and migration in breast cancer cell MCF-7

2014 ◽  
Vol 54 (10) ◽  
pp. 1159-1171 ◽  
Author(s):  
Tian Huanna ◽  
Zuo Tao ◽  
Wang Xiangfei ◽  
An Longfei ◽  
Xie Yuanyuan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Tumor Invasion ◽  
Invasion And Migration ◽  
And Migration ◽  
Mcf 7

scholarly journals Mammalian target of rapamycin inhibitors, temsirolimus and torin 1, attenuate stemness-associated properties and expression of mesenchymal markers promoted by phorbol-myristate-acetate and oncostatin-M in glioblastoma cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769592 ◽  
Author(s):  
Goparaju Chandrika ◽  
Kumar Natesh ◽  
Deepak Ranade ◽  
Ashish Chugh ◽  
Padma Shastry
Keyword(s):  
Phorbol Myristate Acetate ◽  
Mammalian Target Of Rapamycin ◽  
Oncostatin M ◽  
Target Of Rapamycin ◽  
Stem Cell Markers ◽  
Signal Transducer ◽  
Glioblastoma Cells ◽  
Myristate Acetate ◽  
Invasion And Migration ◽  
And Migration

The phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin signaling pathway is crucial for tumor survival, proliferation, and progression, making it an attractive target for therapeutic intervention. In glioblastoma, activated mammalian target of rapamycin promotes invasive phenotype and correlates with poor patient survival. A wide range of mammalian target of rapamycin inhibitors are currently being evaluated for cytotoxicity and anti-proliferative activity in various tumor types but are not explored sufficiently for controlling tumor invasion and recurrence. We recently reported that mammalian target of rapamycin inhibitors—rapamycin, temsirolimus, torin 1, and PP242—suppressed invasion and migration promoted by tumor necrosis factor-alpha and phorbol-myristate-acetate in glioblastoma cells. As aggressive invasion and migration of tumors are associated with mesenchymal and stem-like cell properties, this study aimed to examine the effect of mammalian target of rapamycin inhibitors on these features in glioblastoma cells. We demonstrate that temsirolimus and torin 1 effectively reduced the constitutive as well as phorbol-myristate-acetate/oncostatin-M-induced expression of mesenchymal markers (fibronectin, vimentin, and YKL40) and neural stem cell markers (Sox2, Oct4, nestin, and mushashi1). The inhibitors significantly abrogated the neurosphere-forming capacity induced by phorbol-myristate-acetate and oncostatin-M. Furthermore, we demonstrate that the drugs dephosphorylated signal transducer and activator transcription factor 3, a major regulator of mesenchymal and neural stem cell markers implicating the role of signal transducer and activator transcription factor 3 in the inhibitory action of these drugs. The findings demonstrate the potential of mammalian target of rapamycin inhibitors as “stemness-inhibiting drugs” and a promising therapeutic approach to target glioma stem cells.

scholarly journals Inhibition of tumor–microenvironment interaction and tumor invasion by small-molecule allosteric inhibitor of DDR2 extracellular domain

2018 ◽  
Vol 115 (33) ◽  
pp. E7786-E7794 ◽  
Author(s):  
Whitney R. Grither ◽  
Gregory D. Longmore
Keyword(s):  
Small Molecule ◽  
Tumor Invasion ◽  
Cancer Metastasis ◽  
Metastatic Breast ◽  
Extracellular Domain ◽  
Breast Cancer Metastasis ◽  
Invasion And Migration ◽  
Molecule Inhibitor ◽  
Ligand Interactions ◽  
And Migration

The action of the collagen binding receptor tyrosine kinase (RTK) discoidin domain receptor 2 (DDR2) in both tumor and tumor stromal cells has been established as critical for breast cancer metastasis. Small molecule inhibitors that target the extracellular domain of RTKs are rare, as they have classically been regarded as too small to block binding with large polypeptide ligands. Here, we report the identification and characterization of a selective, extracellularly acting small molecule inhibitor (WRG-28) of DDR2 that uniquely inhibits receptor–ligand interactions via allosteric modulation of the receptor. By targeting DDR2, WRG-28 inhibits tumor invasion and migration, as well as tumor-supporting roles of the stroma, and inhibits metastatic breast tumor cell colonization in the lungs. These findings represent an approach to inhibiting tumor–stromal interactions and support the development of allosteric inhibitors of DDR2, such as WRG-28, as a promising approach to antimetastasis treatment.

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