scholarly journals The Acyclic Retinoid Peretinoin Inhibits Hepatitis C Virus Replication and Infectious Virus Release in Vitro

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Tetsuro Shimakami ◽  
Masao Honda ◽  
Takayoshi Shirasaki ◽  
Riuta Takabatake ◽  
Fanwei Liu ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Infectious Virus ◽  
Virus Release ◽  
Acyclic Retinoid ◽  
Release In Vitro

scholarly journals Efficacy of a Peruvian Botanical Remedy (Sabell A4+) for Treating Liver Disease and Protecting Gastric Mucosal Integrity

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Mark G. Swain ◽  
John L. Wallace ◽  
D. Lorne Tyrrell ◽  
José Cabanillas ◽  
Steven K. H. Aung ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Liver Disease ◽  
Hepatitis C ◽  
Gastric Mucosa ◽  
Virus Replication ◽  
Positive Interactions ◽  
Mucosal Integrity ◽  
Immune Mediated ◽  
Anti Inflammatory

The purpose of this study was to determine the efficacy of a Peruvian botanical formulation for treating disorders of hepatic function and gastric mucosal integrity. The formulation A4+ (Sabell Corporation) contains extracts of Curcuma longa rhizome, Cordia lutea flower, and Annona muricata leaf. Individually these plants have been used as traditional remedies for liver disease. We report the efficacy of A4+ and its components using a variety of in vitro and in vivo disease models. The methods used included tests for antioxidant, anti-inflammatory, and antiviral activity as well as mouse models of liver disease, including Concanavalin A-induced immune-mediated hepatitis and a bile duct ligation model for evaluating sickness behaviour associated with liver disease. Rat models were used to evaluate the gastric mucosal protective property of A4+ following indomethacin challenge and to evaluate its anti-inflammatory action in an “air pouch” model. In all tests, A4+ proved to be more effective than placebo. A4+ was antioxidant and anti-inflammatory and diminished Hepatitis C virus replication in vitro. In animal models, A4+ was shown to protect the liver from immune-mediated hepatitis, improve behavioural function in animals with late stage liver disease, and protect the rat gastric mucosa from ulceration following NSAID exposure. We conclude that A4+ ameliorated many aspects of liver injury, inhibited hepatitis C virus replication, and protected the gastric mucosa from NSAIDs. These varied beneficial properties appear to result from positive interactions between the three constituent herbs.

scholarly journals Caffeine inhibits hepatitis C virus replication in vitro

2014 ◽  
Vol 160 (2) ◽  
pp. 399-407 ◽  
Author(s):  
Mariana N. Batista ◽  
Bruno M. Carneiro ◽  
Ana Cláudia S. Braga ◽  
Paula Rahal
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication

Establishment of serum derived infectivity coculture model for enhancement of hepatitis C virus replication in vitro

2019 ◽  
Vol 27 (3) ◽  
pp. 185-191
Author(s):  
Mariam Mounir ◽  
Marwa Khalil Ibrahim ◽  
Reham M. Dawood ◽  
Ahmed B. Barakat ◽  
Mostafa K. El Awady
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication ◽  
Coculture Model

scholarly journals Hepatitis C Virus p7 and NS2 Proteins Are Essential for Production of Infectious Virus

2007 ◽  
Vol 81 (16) ◽  
pp. 8374-8383 ◽  
Author(s):  
Christopher T. Jones ◽  
Catherine L. Murray ◽  
Dawnnica K. Eastman ◽  
Jodie Tassello ◽  
Charles M. Rice
Keyword(s):  
Life Cycle ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Infectious Virus ◽  
Early Stage ◽  
Virus Production ◽  
Health Concern ◽  
Cell Culture Systems ◽  
Catalytic Active Site

ABSTRACT Hepatitis C virus (HCV) infection is a global health concern affecting an estimated 3% of the world's population. Recently, cell culture systems have been established, allowing recapitulation of the complete virus life cycle for the first time. Since the HCV proteins p7 and NS2 are not predicted to be major components of the virion, nor are they required for RNA replication, we investigated whether they might have other roles in the viral life cycle. Here we utilize the recently described infectious J6/JFH chimera to establish that the p7 and NS2 proteins are essential for HCV infectivity. Furthermore, unprocessed forms of p7 and NS2 were not required for this activity. Mutation of two conserved basic residues, previously shown to be important for the ion channel activity of p7 in vitro, drastically impaired infectious virus production. The protease domain of NS2 was required for infectivity, whereas its catalytic active site was dispensable. We conclude that p7 and NS2 function at an early stage of virion morphogenesis, prior to the assembly of infectious virus.

scholarly journals Stability of CXCL‐8 and Related AU‐Rich mRNAs in the Context of Hepatitis C Virus Replication In Vitro

2006 ◽  
Vol 193 (6) ◽  
pp. 802-811 ◽  
Author(s):  
Jamison Green ◽  
Khalid S. A. Khabar ◽  
Bon Chang A. Koo ◽  
Bryan R. G. Williams ◽  
Stephen J. Polyak
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Replication
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