scholarly journals High-throughput identification and dendritic cell-based functional validation of MHC class I-restricted Mycobacterium tuberculosis epitopes

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Smita K. Nair ◽  
Georgia D. Tomaras ◽  
Ana Paula Sales ◽  
David Boczkowski ◽  
Cliburn Chan ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Dendritic Cell ◽  
Mhc Class I ◽  
High Throughput ◽  
Class I ◽  
Functional Validation

scholarly journals Identification of MHC class II restricted T-cell-mediated reactivity against MHC class I binding Mycobacterium tuberculosis peptides

Immunology ◽  
2011 ◽  
Vol 132 (4) ◽  
pp. 482-491 ◽  
Author(s):  
Mingjun Wang ◽  
Sheila T. Tang ◽  
Anette Stryhn ◽  
Sune Justesen ◽  
Mette V. Larsen ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Cell ◽  
Mhc Class I ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Class I

scholarly journals Dendritic cell maturation in HCV infection: Altered regulation of MHC class I antigen processing-presenting machinery

2014 ◽  
Vol 61 (2) ◽  
pp. 242-251 ◽  
Author(s):  
Patrizia Leone ◽  
Mariangela Di Tacchio ◽  
Simona Berardi ◽  
Teresa Santantonio ◽  
Massimo Fasano ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Mhc Class I ◽  
Antigen Processing ◽  
Dendritic Cell Maturation ◽  
Hcv Infection ◽  
Class I ◽  
Cell Maturation ◽  
Mhc Class I Antigen ◽  
Altered Regulation

Predicting promiscuous antigenic T cell epitopes of Mycobacterium tuberculosis mymA operon proteins binding to MHC Class I and Class II molecules

2016 ◽  
Vol 44 ◽  
pp. 182-189 ◽  
Author(s):  
Iti Saraav ◽  
Kirti Pandey ◽  
Monika Sharma ◽  
Swati Singh ◽  
Prasun Dutta ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Cell ◽  
Mhc Class I ◽  
Class Ii ◽  
Class I ◽  
T Cell Epitopes

scholarly journals The MHC class I peptide repertoire is molded by the transcriptome

2008 ◽  
Vol 205 (3) ◽  
pp. 595-610 ◽  
Author(s):  
Marie-Hélène Fortier ◽  
Étienne Caron ◽  
Marie-Pierre Hardy ◽  
Grégory Voisin ◽  
Sébastien Lemieux ◽  
...  
Keyword(s):  
Mhc Class I ◽  
High Throughput ◽  
Class I ◽  
High Throughput Analysis ◽  
Mhc I ◽  
Cyclin Dependent Kinases ◽  
Histocompatibility Complex ◽  
Accurate Definition ◽  
Primary Mouse ◽  
Definition Of

Under steady-state conditions, major histocompatibility complex (MHC) I molecules are associated with self-peptides that are collectively referred to as the MHC class I peptide (MIP) repertoire. Very little is known about the genesis and molecular composition of the MIP repertoire. We developed a novel high-throughput mass spectrometry approach that yields an accurate definition of the nature and relative abundance of unlabeled peptides presented by MHC I molecules. We identified 189 and 196 MHC I–associated peptides from normal and neoplastic mouse thymocytes, respectively. By integrating our peptidomic data with global profiling of the transcriptome, we reached two conclusions. The MIP repertoire of primary mouse thymocytes is biased toward peptides derived from highly abundant transcripts and is enriched in peptides derived from cyclins/cyclin-dependent kinases and helicases. Furthermore, we found that ∼25% of MHC I–associated peptides were differentially expressed on normal versus neoplastic thymocytes. Approximately half of those peptides are derived from molecules directly implicated in neoplastic transformation (e.g., components of the PI3K–AKT–mTOR pathway). In most cases, overexpression of MHC I peptides on cancer cells entailed posttranscriptional mechanisms. Our results show that high-throughput analysis and sequencing of MHC I–associated peptides yields unique insights into the genesis of the MIP repertoire in normal and neoplastic cells.

Evaluation of T-cell responses to peptides with MHC class I-binding motifs derived from PE_PGRS 33 protein of Mycobacterium tuberculosis

2007 ◽  
Vol 56 (4) ◽  
pp. 466-474 ◽  
Author(s):  
M. G. Chaitra ◽  
M. S. Shaila ◽  
R. Nayak
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Cell ◽  
Mhc Class I ◽  
Class I ◽  
Interleukin 4 ◽  
Specific Response ◽  
T Cell Epitopes ◽  
Cytotoxic Lymphocyte ◽  
Cell Responses ◽  
High Affinity

The PE and PPE proteins of Mycobacterium tuberculosis form a source of antigenic variation among different strains of M. tuberculosis. One of the PE_PGRS proteins, Rv1818c, plays a role in the pathogenesis of mycobacterial infection and specifically influences host-cell responses to tuberculosis infection. Although little is known about these two classes of protein, an immunoinformatics approach has indicated the possibility of their participation in eliciting a major histocompatibility complex (MHC) class I-mediated immune response against tuberculosis, as peptides derived from Rv1818c are predicted to bind to MHC class I molecules with high affinity. In the present work, a DNA vaccine was constructed encoding the full-length Rv1818c protein of M. tuberculosis and its immunogenicity was analysed in BALB/c mice. Immunization with Rv1818c DNA induced a strong CD8+ cytotoxic lymphocyte and Th1-type response, with high levels of gamma interferon (IFN-γ) and low levels of interleukin-4. Two nonameric peptides (Peptide6–14 and Peptide385–393) from Rv1818c were identified by their ability to induce the production of IFN-γ by CD8+ T cells in mice immunized with Rv1818c DNA. An epitope-specific response was demonstrated by the lysis of peptide-pulsed antigen-presenting cells, release of cytotoxic granules and IFN-γ production. These peptides bound with high affinity to MHC H-2Kd and showed low dissociation rates of peptide–MHC complexes. These results could form the basis for testing the identified T-cell epitopes of PE_PGRS proteins in the induction of protective immunity against M. tuberculosis challenge in the mouse model.

scholarly journals Exosomes as Potent Cell-Free Peptide-Based Vaccine. I. Dendritic Cell-Derived Exosomes Transfer Functional MHC Class I/Peptide Complexes to Dendritic Cells

2004 ◽  
Vol 172 (4) ◽  
pp. 2126-2136 ◽  
Author(s):  
Fabrice André ◽  
Nathalie Chaput ◽  
Nöel E. C. Schartz ◽  
Caroline Flament ◽  
Nathalie Aubert ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Dendritic Cell ◽  
Mhc Class I ◽  
Class I ◽  
Free Peptide ◽  
Peptide Complexes
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