scholarly journals AtbHLH29 of Arabidopsis thaliana is a functional ortholog of tomato FER involved in controlling iron acquisition in strategy I plants

Cell Research ◽  
2005 ◽  
Vol 15 (8) ◽  
pp. 613-621 ◽  
Author(s):  
You Xi YUAN ◽  
Juan ZHANG ◽  
Dao Wen WANG ◽  
Hong Qing LING
Keyword(s):  
Arabidopsis Thaliana ◽  
Iron Acquisition ◽  
Strategy I

Comparison of iron acquisition from Fe–pyoverdine by strategy I and strategy II plants

Botany ◽  
2011 ◽  
Vol 89 (10) ◽  
pp. 731-735 ◽  
Author(s):  
Matt Shirley ◽  
Laure Avoscan ◽  
Eric Bernaud ◽  
Gérard Vansuyt ◽  
Philippe Lemanceau
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Species ◽  
Iron Uptake ◽  
Iron Acquisition ◽  
Iron Status ◽  
Iron Nutrition ◽  
Perennial Species ◽  
Major Class ◽  
Graminaceous Plants ◽  
Strategy I

Iron is an essential micronutrient for plants and associated microorganisms. However, the bioavailability of iron in cultivated soils is low. Plants and microorganisms have thus evolved active strategies of iron uptake. Two different iron uptake strategies have been described in dicotyledonous and monocotyledonous graminaceous species. In bacteria, this strategy relies on the synthesis of siderophores. Pyoverdines, a major class of siderophores produced by fluorescent pseudomonads, were previously shown to promote iron nutrition of the dicotyledonous species Arabidopsis thaliana L. (Heynh.), whereas contradictory reports were made on the contribution of those siderophores to the nutrition of graminaceous annuals. Furthermore, no information has so far been available on graminaceous perennials. Here, the contribution of purified pyoverdine of Pseudomonas fluorescens C7R12 to the iron nutrition of two annual and perennial graminaceous plants was assessed and compared with that of two dicotyledonous plant species. Fe–Pyoverdine promoted the iron status of all plant species tested. With the exception of wheat, this promotion was more dramatic in graminaceous species than in dicotyledonous species and was the highest in fescue, a perennial species. The incorporation of 15N-labeled pyoverdine was consistent with the effect on the iron status of the plants tested.

scholarly journals Iron Acquisition from Fe-Pyoverdine by Arabidopsis thaliana

2007 ◽  
Vol 20 (4) ◽  
pp. 441-447 ◽  
Author(s):  
Gérard Vansuyt ◽  
Agnès Robin ◽  
Jean-François Briat ◽  
Catherine Curie ◽  
Philippe Lemanceau
Keyword(s):  
Arabidopsis Thaliana ◽  
Iron Content ◽  
Reductase Activity ◽  
Iron Acquisition ◽  
Iron Nutrition ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wild Type ◽  
In Planta ◽  
Chlorophyll Contents ◽  
The Impact

Taking into account the strong iron competition in the rhizosphere and the high affinity of pyoverdines for Fe(III), these molecules are expected to interfere with the iron nutrition of plants, as they do with rhizospheric microbes. The impact of Fe-pyoverdine on iron content of Arabidopsis thaliana was compared with that of Fe-EDTA. Iron chelated to pyoverdine was incorporated in a more efficient way than when chelated to EDTA, leading to increased plant growth of the wild type. A transgenic line of A. thaliana overexpressing ferritin showed a higher iron content than the wild type when supplemented with Fe-EDTA but a lower iron content when supplemented with Fe-pyoverdine despite its increased reductase activity, suggesting that this activity was not involved in the iron uptake from pyoverdine. A mutant knockout iron transporter IRT1 showed lower iron and chlorophyll contents when supplemented with Fe-EDTA than the wild type but not when supplemented with Fe-pyoverdine, indicating that, in contrast to iron from EDTA, iron from pyoverdine was not incorporated through the IRT1 transporter. Altogether these data suggest that iron from Fe-pyoverdine was not incorporated in planta through the strategy I, which is based on reductase activity and IRT1 transporter. This is supported by the presence of pyoverdine in planta as shown by enzyme-linked immunosorbent assay and by tracing 15N of 15N-pyoverdine.

scholarly journals Uptake of Fe-fraxetin complexes, an IRT1 independent strategy for iron acquisition in Arabidopsis thaliana.

2021 ◽  
Author(s):  
Kevin ROBE ◽  
max STASSEN ◽  
joseph CHAMIEH ◽  
philippe GONZALEZ ◽  
sonia HEM ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Iron Uptake ◽  
Iron Acquisition ◽  
Grass Species ◽  
Alkaline Soil ◽  
Alkaline Soils ◽  
Fe Acquisition ◽  
Dependent Mechanism ◽  
Exogenous Supply

Iron (Fe) is a micronutrient essential for plant growth and development. Iron uptake in alkaline soil is a challenge for most plants. In this study, we investigated the role of the catechol coumarins fraxetin and esculetin in plant Fe acquisition and their Fe chelating properties. Mass spectrometry and capillary electrophoresis were used to characterize Fe-coumarin complexes. To understand the role of these complexes, genetic, molecular and biochemical approaches were deployed. We demonstrated that catechol coumarins are taken up by Arabidopsis thaliana root via an ATP dependent mechanism and that plants defective in IRT1 activity (the main high affinity Fe importer) or bHLH121 (a key regulator of Fe deficiency responses) can be complemented by exogenous supply of fraxetin and to a lesser extent of esculetin. We also showed that Fe and fraxetin can form stable complexes at neutral to alkaline pH that can be taken up by the plant. Overall, these results indicate that at high pH, fraxetin can improve Fe nutrition by directly transporting Fe(III) into the root, circumventing the FRO2/IRT1 system, in a similar way as phytosiderophores do in grasses. This strategy may explain how non-grass species can thrive in alkaline soils.

scholarly journals Metabolome Analysis of Arabidopsis thaliana Roots Identifies a Key Metabolic Pathway for Iron Acquisition

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e102444 ◽  
Author(s):  
Holger Schmidt ◽  
Carmen Günther ◽  
Michael Weber ◽  
Cornelia Spörlein ◽  
Sebastian Loscher ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Metabolic Pathway ◽  
Iron Acquisition ◽  
Metabolome Analysis

Shoot to root communication is necessary to control the expression of iron-acquisition genes in Strategy I plants

Planta ◽  
2012 ◽  
Vol 237 (1) ◽  
pp. 65-75 ◽  
Author(s):  
María J. García ◽  
Francisco J. Romera ◽  
Minviluz G. Stacey ◽  
Gary Stacey ◽  
Eduardo Villar ◽  
...  
Keyword(s):  
Iron Acquisition ◽  
Strategy I

Hypoxia and bicarbonate could limit the expression of iron acquisition genes in Strategy I plants by affecting ethylene synthesis and signaling in different ways

2013 ◽  
Vol 150 (1) ◽  
pp. 95-106 ◽  
Author(s):  
María J. García ◽  
María J. García-Mateo ◽  
Carlos Lucena ◽  
Francisco J. Romera ◽  
Carmen L. Rojas ◽  
...  
Keyword(s):  
Iron Acquisition ◽  
Ethylene Synthesis ◽  
Strategy I

Arthrobacter agilis UMCV2 induces iron acquisition in Medicago truncatula (strategy I plant) in vitro via dimethylhexadecylamine emission

2012 ◽  
Vol 362 (1-2) ◽  
pp. 51-66 ◽  
Author(s):  
Ma del Carmen Orozco-Mosqueda ◽  
Crisanto Velázquez-Becerra ◽  
Lourdes I. Macías-Rodríguez ◽  
Gustavo Santoyo ◽  
Idolina Flores-Cortez ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Iron Acquisition ◽  
Arthrobacter Agilis ◽  
Strategy I

scholarly journals Commensal Pseudomonas protect Arabidopsis thaliana from a coexisting pathogen via multiple lineage-dependent mechanisms

2021 ◽  
Author(s):  
Or Shalev ◽  
Haim Ashkenazy ◽  
Manuela Neumann ◽  
Detlef Weigel
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Protection ◽  
Iron Acquisition ◽  
Protective Role ◽  
Gene Inactivation ◽  
Functional Differences ◽  
Genome Wide ◽  
Bacterial Genes ◽  
Lineage Specific Genes

AbstractPlants are protected from pathogens not only by their own immunity but often also by colonizing commensal microbes. In Arabidopsis thaliana, a group of cryptically pathogenic Pseudomonas strains often dominates local populations. This group coexists in nature with commensal Pseudomonas strains that can blunt the deleterious effects of the pathogens in the laboratory. We have investigated the interaction between one of the Pseudomonas pathogens and 99 naturally co-occurring commensals, finding plant protection to be common among non-pathogenic Pseudomonas. While protective ability is enriched in one specific lineage, there is also a substantial variation for this trait among isolates of this lineage. These functional differences do not align with core-genome phylogenies, suggesting repeated gene inactivation or loss as causal. Using genome-wide association, we discovered that different bacterial genes are linked to plant protection in each lineage. We validated a protective role of several lineage-specific genes by gene inactivation, highlighting iron acquisition and biofilm formation as prominent mechanisms of plant protection in this Pseudomonas lineage. Collectively, our work illustrates the importance of functional redundancy in plant protective traits across an important group of commensal bacteria.

Differences between two green algae in biological availability of iron bound to strong chelators

2006 ◽  
Vol 84 (3) ◽  
pp. 400-411 ◽  
Author(s):  
Harold G. Weger ◽  
Carlyn J. Matz ◽  
Rachel S. Magnus ◽  
Crystal N. Walker ◽  
Michael B. Fink ◽  
...  
Keyword(s):  
Green Algae ◽  
Green Alga ◽  
Vascular Plants ◽  
Iron Acquisition ◽  
Algal Species ◽  
Desferrioxamine B ◽  
Green Algal ◽  
Uptake Rates ◽  
Green Alga Chlorella ◽  
Strategy I

N,N′-di(2-hydroxybenzoyl)-ethylenediamine-N,N′-diacetic acid (HBED) is a very strong Fe3+ chelator. Strategy I vascular plants, which use a reductive system for iron acquisition, similar to many green algae, are able to access iron from HBED (R.L. Chaney. 1988. J. Plant Nutr. 11: 1033–1050). However, iron-limited cells of the Strategy I green alga Chlamydomonas reinhardtii Dangeard were unable to access iron present as Fe3+–HBED. In contrast, Fe3+ chelated with hydroxyethylethylenediaminetriacetic acid (HEDTA; a weaker chelator) was rapidly taken up by iron-limited Chlamydomonas cells. Chlamydomonas ferric reduction rates with Fe3+–HBED were approximately 15% of the rate observed with Fe3+–HEDTA, suggesting that low reduction rates with Fe3+–HBED might be one factor in the low rate of iron acquisition. By contrast, iron-limited cells of the Strategy I green alga Chlorella kessleri Fott et Nováková were able to rapidly assimilate Fe3+ chelated by HBED, although ferric reduction rates with Fe3+–HBED were approximately 38% the rate of activity with Fe3+–HEDTA. Similar differential iron uptake rates for the two algal species were obtained using the strong Fe3+ chelator (and siderophore analogue) desferrioxamine B mesylate and the cyanobacterial siderophore schizokinen. These results suggest that there are differences among Strategy I green algae in their abilities to acquire Fe3+ from various ferric chelates: not all Strategy I algae can equally access tightly complexed Fe3+. Chlamydomonas appears to be the first documented Strategy I organism that is unable to acquire iron from Fe3+–HBED. These results also suggest that green algal iron acquisition from siderophores is species dependent. Finally, we suggest that iron acquisition from Fe3+–HBED might serve as an assay for an organisms’ ability to access tightly complexed iron.

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