scholarly journals Analysis of redox regulation of cytochrome c-induced apoptosis in a cell-free system

1999 ◽  
Vol 6 (7) ◽  
pp. 683-688 ◽  
Author(s):  
Zhaohui Pan ◽  
David W Voehringer ◽  
Raymond E Meyn
Keyword(s):  
Cytochrome C ◽  
Redox Regulation ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Induced Apoptosis

A Cytofluorometric Assay of Nuclear Apoptosis Induced in a Cell-Free System: Application to Ceramide-Induced Apoptosis

1997 ◽  
Vol 236 (2) ◽  
pp. 397-403 ◽  
Author(s):  
Santos A. Susin ◽  
Naoufal Zamzami ◽  
Nathanael Larochette ◽  
Bruno Dallaporta ◽  
Isabel Marzo ◽  
...  
Keyword(s):  
System Application ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Induced Apoptosis ◽  
Nuclear Apoptosis

Reconstitution of electron transport by cytochrome c-553 in a cell-free system of Nostoc muscorum

1982 ◽  
Vol 3 (3) ◽  
pp. 191-201 ◽  
Author(s):  
Erwin St�rzl ◽  
Siegfried Scherer ◽  
Peter B�ger
Keyword(s):  
Electron Transport ◽  
Cytochrome C ◽  
Nostoc Muscorum ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells

Sensors ◽  
2020 ◽  
Vol 20 (6) ◽  
pp. 1782 ◽  
Author(s):  
Azarakhsh Oladzad ◽  
Maryam Nikkhah ◽  
Saman Hosseinkhani
Keyword(s):  
Cytochrome C ◽  
Caspase 3 ◽  
Luciferase Activity ◽  
Early Stage ◽  
Apoptosis Induction ◽  
Free System ◽  
Biological Tool ◽  
A Cell ◽  
Induced Apoptosis ◽  
Split Luciferase

The apoptotic protease-activating factor 1 (Apaf-1) split luciferase biosensor has been used as a biological tool for the detection of early stage of apoptosis. The effect of doxorubicin in a cell-based assay and the addition of cytochrome c and ATP in a cell-free system have been used to test the functionality of the reporter for the detection of apoptosome formation. Here, our data established a drug- and cytochrome c/ATP-independent way of apoptosis induction relying on the expression of the biosensor itself to induce formation of apoptosome. Overexpression of Apaf-1 constructs led to increased split luciferase activity and caspase-3 activity in the absence of any drug treatment. Caspase-3 activity was significantly inhibited when caspase-9DN was co-overexpressed, while the activity of the Apaf1 biosensor was significantly increased. Our results show that the Apaf-1 biosensor does not detect etoposide-induced apoptosis.

scholarly journals FLICE Induced Apoptosis in a Cell-free System

1997 ◽  
Vol 272 (5) ◽  
pp. 2952-2956 ◽  
Author(s):  
Marta Muzio ◽  
Guy S. Salvesen ◽  
Vishva M. Dixit
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Induced Apoptosis

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell
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