scholarly journals Structural and functional insights into sorting nexin 5/6 interaction with bacterial effector IncE

2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Qingxiang Sun ◽  
Xin Yong ◽  
Xiaodong Sun ◽  
Fan Yang ◽  
Zhonghua Dai ◽  
...  
Keyword(s):  
Sorting Nexin ◽  
Sorting Nexin 5

scholarly journals Interaction of Human Cytomegalovirus Tegument Proteins ppUL35 and ppUL35A with Sorting Nexin 5 Regulates Glycoprotein B (gpUL55) Localization

2018 ◽  
Vol 92 (9) ◽  
Author(s):  
Gregor Maschkowitz ◽  
Sabine Gärtner ◽  
Heike Hofmann-Winkler ◽  
Helmut Fickenscher ◽  
Michael Winkler
Keyword(s):  
Human Cytomegalovirus ◽  
Retrograde Transport ◽  
Viral Assembly ◽  
Glycoprotein B ◽  
Tegument Protein ◽  
Immunocompromised Patients ◽  
Structural Protein ◽  
Transport Pathway ◽  
Sorting Nexin ◽  
Sorting Nexin 5

ABSTRACTHuman cytomegalovirus (HCMV) is a widespread human pathogen that causes asymptomatic infection in healthy individuals but poses a serious threat to immunocompromised patients. During the late phase of HCMV infection, the viral capsid is transported to the cytoplasmic viral assembly center (cVAC), where it is enclosed by the tegument protein layer and the viral envelope. The cVAC consists of circularly arranged vesicles from thetrans-Golgi and endosomal networks. The HCMV gene UL35 encodes ppUL35 and its shorter form, ppUL35A. We have previously shown that the UL35 gene is involved in HCMV assembly, but it is unknown how UL35 proteins regulate viral assembly. Here we show that sorting nexin 5 (SNX5), a component of the retromer and part of the retrograde transport pathway, interacts with UL35 proteins. Expression of wild-type proteins but not mutants defective in SNX5 binding resulted in the cellular redistribution of the cation-independent mannose-6-phosphate receptor (CI-M6PR), indicating that UL35 proteins bind and negatively regulate SNX5 to modulate cellular transport pathways. Furthermore, binding of UL35 proteins to SNX5 was required for efficient viral replication and for transport of the most abundant HCMV glycoprotein B (gB; gpUL55) to the cVAC. These results indicate that ppUL35 and ppUL35A control the localization of the essential gB through the regulation of a retrograde transport pathway. Thus, this work is the first to define a molecular interaction between a tegument protein and a vesicular transport factor to regulate glycoprotein localization.IMPORTANCEHuman cytomegalovirus is ubiquitously present in the healthy population, but reactivation or reinfection can cause serious, life-threatening infections in immunocompromised patients. For completion of its lytic cycle, human cytomegalovirus induces formation of an assembly center where mature virus particles are formed from multiple viral proteins. Viral glycoproteins use separate vesicular pathways for transport to the assembly center, which are incompletely understood. Our research identified a viral structural protein which affects the localization of one of the major glycoproteins. We could link this change in glycoprotein localization to an interaction of the structural protein with a cellular protein involved in regulation of vesicle transport. This increases our understanding of how the virus intersects into cellular regulatory pathways to enhance its own replication.

Sorting nexin‐5 is not required for normal spermatogenesis and male fertilization in mice

2020 ◽  
Vol 87 (12) ◽  
pp. 1202-1205
Author(s):  
Qi Zhou ◽  
Zhenyu Wang ◽  
Neha Nayak ◽  
Mark Subler ◽  
Zhibing Zhang
Keyword(s):  
Sorting Nexin ◽  
Normal Spermatogenesis ◽  
Sorting Nexin 5

scholarly journals Sorting Nexin 5 Controls Head and Neck Squamous Cell Carcinoma Progression by Modulating FBW7

2019 ◽  
Vol 10 (13) ◽  
pp. 2942-2952 ◽  
Author(s):  
Jinyang Cai ◽  
Ming Sun ◽  
Bin Hu ◽  
Brad Windle ◽  
Xin Ge ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Sorting Nexin ◽  
Sorting Nexin 5

scholarly journals Sorting nexin 5 of a new diagnostic marker of papillary thyroid carcinoma regulates Caspase-2

2012 ◽  
Vol 103 (7) ◽  
pp. 1356-1362 ◽  
Author(s):  
Shihoko Ara ◽  
Tomoki Kikuchi ◽  
Hiroshi Matsumiya ◽  
Takashi Kojima ◽  
Terufumi Kubo ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Diagnostic Marker ◽  
Papillary Thyroid ◽  
Sorting Nexin ◽  
Caspase 2 ◽  
Sorting Nexin 5

Loss of sorting nexin 5 stabilizes internalized growth factor receptors to promote thyroid cancer progression

2017 ◽  
Vol 243 (3) ◽  
pp. 342-353 ◽  
Author(s):  
Sumito Jitsukawa ◽  
Ryuta Kamekura ◽  
Koji Kawata ◽  
Fumie Ito ◽  
Akinori Sato ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Growth Factor ◽  
Cancer Progression ◽  
Growth Factor Receptors ◽  
Sorting Nexin ◽  
Sorting Nexin 5

scholarly journals Novel role of sorting nexin 5 in renal D 1 dopamine receptor trafficking and function: implications for hypertension

2012 ◽  
Vol 27 (5) ◽  
pp. 1808-1819 ◽  
Author(s):  
Van Anthony M. Villar ◽  
Ines Armando ◽  
Hironobu Sanada ◽  
Lauren C. Frazer ◽  
Christen M. Russo ◽  
...  
Keyword(s):  
Dopamine Receptor ◽  
Receptor Trafficking ◽  
Sorting Nexin ◽  
And Function ◽  
Sorting Nexin 5

scholarly journals The phosphorylation of sorting nexin 5 at serine 226 regulates retrograde transport and macropinocytosis

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0207205 ◽  
Author(s):  
Nao Itai ◽  
Tsukasa Shimazu ◽  
Takayuki Kimura ◽  
Issei Ibe ◽  
Ryo Yamashita ◽  
...  
Keyword(s):  
Retrograde Transport ◽  
Sorting Nexin ◽  
Sorting Nexin 5

scholarly journals Sorting nexin 5 selectively regulates dorsal-ruffle-mediated macropinocytosis in primary macrophages

2015 ◽  
Vol 128 (23) ◽  
pp. 4407-4419 ◽  
Author(s):  
J. P. Lim ◽  
P. Gosavi ◽  
J. D. Mintern ◽  
E. M. Ross ◽  
P. A. Gleeson
Keyword(s):  
Sorting Nexin ◽  
Sorting Nexin 5

scholarly journals The Phox Domain of Sorting Nexin 5 Lacks Phosphatidylinositol 3-Phosphate (PtdIns(3)P) Specificity and Preferentially Binds to Phosphatidylinositol 4,5-Bisphosphate (PtdIns(4,5)P2)

2009 ◽  
Vol 284 (35) ◽  
pp. 23697-23707 ◽  
Author(s):  
Leonardus M. I. Koharudin ◽  
William Furey ◽  
Hao Liu ◽  
Yong-Jian Liu ◽  
Angela M. Gronenborn
Keyword(s):  
Sorting Nexin ◽  
Phosphatidylinositol 3 ◽  
Sorting Nexin 5
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