scholarly journals Erratum: Corrigendum: Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
D. D’Esposito ◽  
L. Orrù ◽  
E. Dattolo ◽  
L. Bernardo ◽  
A. Lamontanara ◽  
...  
Keyword(s):  
Simple Sequence Repeat Marker ◽  
Simple Sequence Repeat ◽  
Posidonia Oceanica ◽  
Sequence Repeat ◽  
Marker Discovery ◽  
Simple Sequence

scholarly journals Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
D. D’Esposito ◽  
L. Orrù ◽  
E. Dattolo ◽  
L. Bernardo ◽  
A. Lamontanara ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Population Genetic ◽  
Posidonia Oceanica ◽  
Rna Seq ◽  
Sequence Repeat ◽  
Functional Variation ◽  
Data Set ◽  
Genetic Features ◽  
Marker Discovery ◽  
Simple Sequence

Abstract Posidonia oceanica is an endemic seagrass in the Mediterranean Sea, where it provides important ecosystem services and sustains a rich and diverse ecosystem. P. oceanica meadows extend from the surface to 40 meters depth. With the aim of boosting research in this iconic species, we generated a comprehensive RNA-Seq data set for P. oceanica by sequencing specimens collected at two depths and two times during the day. With this approach we attempted to capture the transcriptional diversity associated with change in light and other depth-related environmental factors. Using this extensive data set we generated gene predictions and identified an extensive catalogue of potential Simple Sequence Repeats (SSR) markers. The data generated here will open new avenues for the analysis of population genetic features and functional variation in P. oceanica. In total, 79,235 contigs were obtained by the assembly of 70,453,120 paired end reads. 43,711 contigs were successfully annotated. A total of 17,436 SSR were identified within 13,912 contigs.

scholarly journals Study of Plant Genetic Variation through Molecular Markers: An Overview

2021 ◽  
pp. 464-473
Author(s):  
Zeina S. M. Al-Hadeithi ◽  
Saade Abdalkareem Jasim
Keyword(s):  
Molecular Markers ◽  
Simple Sequence Repeat Marker ◽  
Fragment Length ◽  
Simple Sequence Repeat ◽  
Plant Biotechnology ◽  
Molecular Techniques ◽  
Basic Material ◽  
Sequence Repeat ◽  
Simple Sequence

This article refers to viewing the role of molecular markers during analyzing the genome of plants and their importance in plant biotechnology. In recent years, we observed the role of molecular techniques in programs for improving plant breeding and preserving genetic resources has been observed, and molecular and biochemical indicators which represent basic material through determining the diversity between genotypes for indicators it is never affected by external surrounding conditions as always in the phenotype features. Molecular markers of DNA have been widely applied to answer a range of questions related to taxonomy, molecular evolution, population genetics, and genetic diversity, as well as monitoring trade in plants and food products , in addition to its having a role in studying gene expression , genetic mapping, and studies of species evolution providing fast and accurate results. In this work, the advantages and limitations of the molecular techniques applied in plant sciences such as: RAPD (Random Amplification Polymorphic DNA Marker); ISSR (Inter Simple Sequence Repeat Marker); SSR (Simple Sequence Repeat Marker); AFLP (Amplified Fragment Length Polymorphic Marker); RFLP (Restriction Fragment Length Polymorphism Marker); SNP (Single Nucleotide Polymorphism) and Real Time PCR.

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