scholarly journals The FANTOM5 collection, a data series underpinning mammalian transcriptome atlases in diverse cell types

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Hideya Kawaji ◽  
Takeya Kasukawa ◽  
Alistair Forrest ◽  
Piero Carninci ◽  
Yoshihide Hayashizaki
Keyword(s):  
Cell Types ◽  
Data Series ◽  
Mammalian Transcriptome

scholarly journals Calculating sample size requirements for temporal dynamics in single cell proteomics

2020 ◽  
Author(s):  
Hannah Boekweg ◽  
Amanda J. Guise ◽  
Edward D. Plowey ◽  
Ryan T. Kelly ◽  
Samuel H. Payne
Keyword(s):  
Single Cell ◽  
Time Course ◽  
Temporal Dynamics ◽  
False Positive Rate ◽  
Cell Types ◽  
State Transitions ◽  
Data Series ◽  
Cell Heterogeneity ◽  
Critical Question ◽  
Measurement Variability

AbstractSingle cell measurements are uniquely capable of characterizing cell-to-cell heterogeneity, and have been used to explore the large diversity of cell types and physiological functions present in tissues and other complex cell assemblies. An intriguing application of single cell proteomics is the characterization of proteome dynamics during biological transitions, like cellular differentiation or disease progression. Time course experiments, which regularly take measurements during state transitions, rely on the ability to detect dynamic trajectories in a data series. However, in a single cell proteomics experiment, cell-to-cell heterogeneity complicates the confident identification of proteome dynamics as measurement variability may be higher than expected. Therefore, a critical question for these experiments is how many data points need to be acquired during the time course to enable robust statistical analysis. We present here an analysis of the most important variables that affect statistical confidence in the detection of proteome dynamics: fold-change, measurement variability, and the number of cells measured during the time course. Importantly, we show that datasets with less than 16 measurements across the time domain suffer from low accuracy and also have a high false-positive rate. We also demonstrate how to balance competing demands in experimental design to achieve a desired result.

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