scholarly journals Preparation of soft tissue samples for synchrotron radiation microtomography and nanotomography

2018 ◽  
Author(s):  
Ryuta Mizutani ◽  
Rino Saiga ◽  
Ryuta Mizutani
Keyword(s):  
Synchrotron Radiation ◽  
Soft Tissue ◽  
Tissue Samples ◽  
Synchrotron Radiation Microtomography

Synchrotron inline phase contrast µCT enables detailed virtual histology of embedded soft-tissue samples with and without staining

2018 ◽  
Vol 25 (4) ◽  
pp. 1153-1161 ◽  
Author(s):  
Mara Saccomano ◽  
Jonas Albers ◽  
Giuliana Tromba ◽  
Marina Dobrivojević Radmilović ◽  
Srećko Gajović ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Soft Tissue ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Strong Increase ◽  
Micro Computed Tomography ◽  
Contrast Imaging ◽  
Specific Staining ◽  
Tissue Samples ◽  
Virtual Histology

Synchrotron radiation micro-computed tomography (SRµCT) based virtual histology, in combination with dedicated ex vivo staining protocols and/or phase contrast, is an emerging technology that makes use of three-dimensional images to provide novel insights into the structure of tissue samples at microscopic resolution with short acquisition times of the order of minutes or seconds. However, the high radiation dose creates special demands on sample preparation and staining. As a result of the lack of specific staining in virtual histology, it can supplement but not replace classical histology. Therefore, the aim of this study was to establish and compare optimized ex vivo staining and acquisition protocols for SRµCT-based virtual histology of soft-tissue samples, which could be integrated into the standard workflow of classical histology. The high grade of coherence of synchrotron radiation allows the application of propagation-based phase contrast imaging (PBI). In this study, PBI yielded a strong increase in image quality even at lower radiation doses and consequently prevented any damage to the tissue samples or the embedding material. This work has demonstrated that the improvement in contrast-to-noise ratio by PBI enabled label-free virtual histology of soft-tissue specimens embedded in paraffin to a level of detail that exceeds that achieved with staining protocols.

Improved middle-ear soft-tissue visualization using synchrotron radiation phase-contrast imaging

2017 ◽  
Vol 354 ◽  
pp. 1-8 ◽  
Author(s):  
Mai Elfarnawany ◽  
Seyed Alireza Rohani ◽  
Soroush Ghomashchi ◽  
Daniel G. Allen ◽  
Ning Zhu ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Soft Tissue ◽  
Middle Ear ◽  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
Radiation Phase

Canine Synovial Sarcoma of the Stifle Joint with Pulmonary and Liver Metastases

2021 ◽  
Vol 38 ◽  
pp. 6-12
Author(s):  
R.J. Samson ◽  
F.H. Mpagike ◽  
A.K. Felix ◽  
A.B. Matondo ◽  
M. Makungu
Keyword(s):  
Soft Tissue ◽  
Lung Metastases ◽  
Synovial Cell ◽  
Proximal Region ◽  
Radiographic Examination ◽  
Tissue Samples ◽  
Stifle Joint ◽  
Mongrel Dog ◽  
Biopsy Examination ◽  
The Right

A seven-year-old female mongrel dog was presented at the Sokoine University of Agriculture Teaching Animal Hospital for second opinion regarding a progressive swelling of the right hind limb of two months duration. Clinical examination revealed a loss of body condition, tachypnea and tachycardia, a painful immobile solid mass of 20 cm x 14 cm x 10 cm located on the right stifle joint, leucocytosis, and anaemia. Radiographic examination of the joint revealed marked soft tissue swelling with amorphous areas of mineralization and complete destruction of the proximal tibia. Smooth and solid periosteal reaction was seen around the tibia and fibula with a Codman’s triangle. Multiple nodules and a mass with soft tissue opacity were seen in the lung fields. Post-mortem examination revealed separation of tibia and fibula, softening of the proximal parts of the tibia, and complete integration of the proximal region of the two bones and articular tissues into the surrounding muscular tissues. Multifocal nodular lesions of variable sizes were mostly found in the lungs and partly in the liver. Histologically, predominantly oval and round cells with variable nuclear sizes and moderate mitoses were observed in tissue samples from the joint, lungs and the liver although some parts of the lung metastases showed both round and spindle shaped tumor cells. Clinical profile is suggestive of aggressive biphasic (spindle and epithelial) type of synovial cell sarcoma with lung and liver metastasis. Early radiographic and biopsy examination of persistent musculoskeletal nodules is recommended for early diagnosis and interventions.

Nondestructive imaging of the internal microstructure of vessels and nerve fibers in rat spinal cord using phase-contrast synchrotron radiation microtomography

2017 ◽  
Vol 24 (2) ◽  
pp. 482-489 ◽  
Author(s):  
Jianzhong Hu ◽  
Ping Li ◽  
Xianzhen Yin ◽  
Tianding Wu ◽  
Yong Cao ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Nerve Fibers ◽  
The Body ◽  
Rat Spinal Cord ◽  
Nondestructive Imaging ◽  
Synchrotron Radiation Microtomography ◽  
The Brain

The spinal cord is the primary neurological link between the brain and other parts of the body, but unlike those of the brain, advances in spinal cord imaging have been challenged by the more complicated and inhomogeneous anatomy of the spine. Fortunately with the advancement of high technology, phase-contrast synchrotron radiation microtomography has become widespread in scientific research because of its ability to generate high-quality and high-resolution images. In this study, this method has been employed for nondestructive imaging of the internal microstructure of rat spinal cord. Furthermore, digital virtual slices based on phase-contrast synchrotron radiation were compared with conventional histological sections. The three-dimensional internal microstructure of the intramedullary arteries and nerve fibers was vividly detected within the same spinal cord specimen without the application of a stain or contrast agent or sectioning. With the aid of image post-processing, an optimization of vessel and nerve fiber images was obtained. The findings indicated that phase-contrast synchrotron radiation microtomography is unique in the field of three-dimensional imaging and sets novel standards for pathophysiological investigations in various neurovascular diseases.

Synchrotron Radiation Microtomography for Large Area 3D Imaging of Multilevel Microelectronic Packages

2014 ◽  
Vol 43 (12) ◽  
pp. 4421-4427 ◽  
Author(s):  
John W. Elmer ◽  
Yan Li ◽  
Holly D. Barth ◽  
Dilworth Y. Parkinson ◽  
Mario Pacheco ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
3D Imaging ◽  
Large Area ◽  
Microelectronic Packages ◽  
Synchrotron Radiation Microtomography

scholarly journals Kinetic Modelling of [68Ga]Ga-DOTA-Siglec-9 in Porcine Osteomyelitis and Soft Tissue Infections

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4094 ◽  
Author(s):  
Lars Jødal ◽  
Anne Roivainen ◽  
Vesa Oikonen ◽  
Sirpa Jalkanen ◽  
Søren B. Hansen ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Hind Limb ◽  
Kinetic Modelling ◽  
Compartment Model ◽  
Cell Surface Expression ◽  
Soft Tissue Infections ◽  
Tissue Samples ◽  
Tissue Compartment ◽  
Arterial Blood ◽  
Kinetics Of

Background: [68Ga]Ga-DOTA-Siglec-9 is a positron emission tomography (PET) radioligand for vascular adhesion protein 1 (VAP-1), a protein involved in leukocyte trafficking. The tracer facilitates the imaging of inflammation and infection. Here, we studied the pharmacokinetic modelling of [68Ga]Ga-DOTA-Siglec-9 in osteomyelitis and soft tissue infections in pigs. Methods: Eight pigs with osteomyelitis and soft tissue infections in the right hind limb were dynamically PET scanned for 60 min along with arterial blood sampling. The fraction of radioactivity in the blood accounted for by the parent tracer was evaluated with radio-high-performance liquid chromatography. One- and two-tissue compartment models were used for pharmacokinetic evaluation. Post-mortem soft tissue samples from one pig were analysed with anti-VAP-1 immunofluorescence. In each analysis, the animal’s non-infected left hind limb was used as a control. Results: Tracer uptake was elevated in soft tissue infections but remained low in osteomyelitis. The kinetics of [68Ga]Ga-DOTA-Siglec-9 followed a reversible 2-tissue compartment model. The tracer metabolized quickly; however, taking this into account, produced more ambiguous results. Infected soft tissue samples showed endothelial cell surface expression of the Siglec-9 receptor VAP-1. Conclusion: The kinetics of [68Ga]Ga-DOTA-Siglec-9 uptake in porcine soft tissue infections are best described by the 2-tissue compartment model.

scholarly journals 3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

10.3791/60251 ◽  
2019 ◽  
Author(s):  
Madleen Busse ◽  
Mark Müller ◽  
Melanie A. Kimm ◽  
Simone Ferstl ◽  
Sebastian Allner ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Soft Tissue ◽  
3D Imaging ◽  
Staining Method ◽  
Specific Staining ◽  
Tissue Samples ◽  
X Ray
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