Extracting high molecular weight genomic DNA from Saccharomyces cerevisiae

2018 ◽  
Author(s):  
Erwan DENIS ◽  
Erwan Denis ◽  
Sophie Sanchez ◽  
Barbara Mairey ◽  
Odette Beluche ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
High Molecular Weight ◽  
Genomic Dna

The 70-kilodalton adenylyl cyclase-associated protein is not essential for interaction of Saccharomyces cerevisiae adenylyl cyclase with RAS proteins

1992 ◽  
Vol 12 (11) ◽  
pp. 4937-4945
Author(s):  
J Wang ◽  
N Suzuki ◽  
T Kataoka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Adenylyl Cyclase ◽  
High Molecular Weight ◽  
Size Difference ◽  
Missense Mutations ◽  
Ras Proteins ◽  
High Molecular Weight Complex ◽  
Ras Protein ◽  
Dependent Activity

In the yeast Saccharomyces cerevisiae, adenylyl cyclase is regulated by RAS proteins. We show here that the yeast adenylyl cyclase forms at least two high-molecular-weight complexes, one with the RAS protein-dependent adenylyl cyclase activity and the other with the Mn(2+)-dependent activity, which are separable by their size difference. The 70-kDa adenylyl cyclase-associated protein (CAP) existed in the former complex but not in the latter. Missense mutations in conserved motifs of the leucine-rich repeats of the catalytic subunit of adenylyl cyclase abolished the RAS-dependent activity, which was accompanied by formation of a very high molecular weight complex having the Mn(2+)-dependent activity. Contrary to previous results, disruption of the gene encoding CAP did not alter the extent of RAS protein-dependent activation of adenylyl cyclase, while a concomitant decrease in the size of the RAS-responsive complex was observed. These results indicate that CAP is not essential for interaction of the yeast adenylyl cyclase with RAS proteins even though it is an inherent component of the RAS-responsive adenylyl cyclase complex.

Calcium Phosphate-Mediated Transfection of Cells with High-Molecular-Weight Genomic DNA

2019 ◽  
Vol 2019 (10) ◽  
pp. pdb.prot095448 ◽  
Author(s):  
Priti Kumar ◽  
Arvindhan Nagarajan ◽  
Pradeep D. Uchil
Keyword(s):  
Molecular Weight ◽  
Calcium Phosphate ◽  
High Molecular Weight ◽  
Genomic Dna

scholarly journals Durable synthesis of high molecular weight heat shock proteins in G0 cells of the yeast and other eucaryotes.

1984 ◽  
Vol 99 (1) ◽  
pp. 199-207 ◽  
Author(s):  
H Iida ◽  
I Yahara
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
High Molecular Weight ◽  
Resting State ◽  
Specific Class ◽  
Embryonic Fibroblasts ◽  
Long Period ◽  
Shock Proteins

We report that eucaryotic cells were induced to synthesize a specific class of heat shock proteins (hsps) when they entered the resting state, G0. This finding was originally made with Saccharomyces cerevisiae cells by taking advantage of the system in which we can distinguish between G1 arrests leading to G0 and those that do not result in G0 (Iida, H., and I. Yahara, 1984, J. Cell Biol. 98:1185-1193). Similar observations were subsequently made with higher eucaryotic cells including chick embryonic fibroblasts (CEF), mouse T lymphocytes, and Drosophila GM1 cells. The induction of hsps in G0 cells was distinct from that in heat-shocked cells in two respects. First, hsps with molecular weight around 25,000 were not induced in G0 cells, whereas most, if not all, high molecular weight (HMW) hsps were commonly induced both in G0 cells and in heat-shocked cells. Second, in contrast to the transient synthesis of hsps in heat-shocked cells, G0 cells continued to synthesize hsps at the stimulated rate for a relatively long period. These results suggest the possibility that high molecular weight hsps might function in a transition from the proliferating state to G0 or in maintaining G0 in the eucaryote.

A Fast and Inexpensive High-Throughput Protocol for Isolating High Molecular Weight Genomic DNA from Lichens

Herzogia ◽  
2016 ◽  
Vol 29 (2) ◽  
pp. 610 ◽  
Author(s):  
Silke Werth ◽  
Sigrún Reynisdóttir ◽  
Hörður Gudmundsson ◽  
Ólafur S. Andrésson
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Throughput ◽  
Genomic Dna
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