scholarly journals Reconstitution of transmembrane protein Na+,K+-ATPase in giant unilamellar vesicles of lipid mixtures involving PSM, DOPC, DPPC and cholesterol at physiological buffer and temperature conditions

2016 ◽  
Author(s):  
Tripta Bhatia ◽  
Tripta Bhatia ◽  
Flemming Cornelius ◽  
Ole G. Mouritsen ◽  
John H. Ipsen
Keyword(s):  
Transmembrane Protein ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Temperature Conditions ◽  
Lipid Mixtures

Effect of flavonoids on the phase separation in giant unilamellar vesicles formed from binary lipid mixtures

2014 ◽  
Vol 178 ◽  
pp. 77-83 ◽  
Author(s):  
Olga S. Ostroumova ◽  
Evgeny G. Chulkov ◽  
Olga V. Stepanenko ◽  
Ludmila V. Schagina
Keyword(s):  
Phase Separation ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Lipid Mixtures

scholarly journals Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls

2021 ◽  
Vol 22 (12) ◽  
pp. 6335
Author(s):  
Mariem Souissi ◽  
Julien Pernier ◽  
Olivier Rossier ◽  
Gregory Giannone ◽  
Christophe Le Clainche ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Membrane ◽  
Transmembrane Protein ◽  
Model Membranes ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Good Practices ◽  
Adhesion Proteins ◽  
Controlled Conditions

Giant unilamellar vesicles (GUV) are powerful tools to explore physics and biochemistry of the cell membrane in controlled conditions. For example, GUVs were extensively used to probe cell adhesion, but often using non-physiological linkers, due to the difficulty of incorporating transmembrane adhesion proteins into model membranes. Here we describe a new protocol for making GUVs incorporating the transmembrane protein integrin using gel-assisted swelling. We report an optimised protocol, enumerating the pitfalls encountered and precautions to be taken to maintain the robustness of the protocol. We characterise intermediate steps of small proteoliposome formation and the final formed GUVs. We show that the integrin molecules are successfully incorporated and are functional.

scholarly journals Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi
Keyword(s):  
Phospholipid Fatty Acid ◽  
Enzymatic Reaction ◽  
Stimuli Responsive ◽  
Membrane Composition ◽  
Giant Unilamellar Vesicles ◽  
Physical Processes ◽  
Unilamellar Vesicles ◽  
Division Process ◽  
The Origin Of Life ◽  
Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1082-1088
Author(s):  
Chiho Kataoka-Hamai ◽  
Kohsaku Kawakami
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles
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