scholarly journals Covalent immobilization of proteins onto photoactivated polystyrene microtiter plates for enzyme-linked immunosorbent assay procedures

2013 ◽  
Author(s):  
Pradip Nahar ◽  
Pradip Nahar
Keyword(s):  
Immunosorbent Assay ◽  
Covalent Immobilization ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtiter Plates

scholarly journals Reevaluation of enzyme-linked immunosorbent assay with ultraviolet-treated polystyrene microtiter plates for measurement of antibodies to dsDNA.

1996 ◽  
Vol 19 (2) ◽  
pp. 163-167
Author(s):  
Junichi Kaburaki ◽  
Takashi Ogasawara ◽  
Masakatsu Hayakawa ◽  
Masataka Kuwana ◽  
Takeshi Tojo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtiter Plates ◽  
Antibodies To Dsdna

scholarly journals An enzyme-linked immunosorbent assay for platelet compatibility testing

Blood ◽  
1983 ◽  
Vol 62 (4) ◽  
pp. 744-749 ◽  
Author(s):  
JD Tamerius ◽  
JG Curd ◽  
P Tani ◽  
R McMillan
Keyword(s):  
Antibody Titer ◽  
Immunosorbent Assay ◽  
Clinical Laboratory ◽  
Clinical Problem ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtiter Plates ◽  
Volunteer Donor ◽  
Antibody Titers ◽  
Test Serum ◽  
Selection Of

Abstract The selection of platelet donors for patients who are refractory to random donor platelets often presents a difficult clinical problem. We describe an enzyme-linked immunosorbent assay (ELISA) for evaluating alloantibodies in refractory patients. Platelets from prospective donors are immobilized on microtiter plates and, after incubation with test serum and washing, platelet-bound IgG is detected with enzyme- linked anti-human IgG. Platelets from 46 prospective donors were tested. Twenty-two were judged compatible (reciprocal of the antibody titer less than 16) and, of these, 15 were used as platelet donors; each gave a measurable platelet increment after transfusion. The magnitude of the response was roughly proportional to the assay results. Platelets from donors giving antibody titers less than 4 resulted in platelet increments at 1 hr ranging from 4,890 to 22,200 (median 12,600), while platelets from donors giving titers of 8 or 16 resulted in lesser increments (550–4548). Conversely, 5 of the 24 patients found incompatible by the assay (titer greater than 16) gave no platelet increment, and in 3 instances, the recipient developed fever and chills after the transfusion. The assay is sensitive, simple, and adaptable to the clinical laboratory. Platelets from volunteer donor panels can be plated and stored for up to 6 mo.

scholarly journals Covalent Immobilization of Antibodies through Tetrazine-TCO Reaction to Improve Sensitivity of ELISA Technique

Biosensors ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 524
Author(s):  
Tania García-Maceira ◽  
Fé I. García-Maceira ◽  
José A. González-Reyes ◽  
Luis A. Torres-Sánchez ◽  
Ana Belén Aragón-Gómez ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Recombinant Protein ◽  
Sandwich Elisa ◽  
Covalent Immobilization ◽  
The Other ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtiter Plates ◽  
Elisa Technique ◽  
Density Surface ◽  
Capture Antibody

Enzyme-linked immunosorbent assay (ELISA) is routinely used to detect biomolecules related to several diseases facilitating diagnosis and monitoring of these, as well as the possibility of decreasing their mortality rate. Several methods have been carried out to improve the ELISA sensitivity through antibodies immobilization on the microtiter plates. Here, we have developed a strategy of antibodies immobilization to improve the ELISA sensitivity increasing the antibody density surface through the tetrazine (Tz)-trans-cyclooctene (TCO) reaction. For this, we prepared surfaces with tetrazine groups while the captured antibody was conjugated with TCO. The tetrazine surfaces were prepared in two different ways: (1) from aminated plates and (2) from Tz-BSA-coated plates. The surfaces were evaluated using two sandwich ELISA models, one of them using the low-affinity antibody anti-c-myc as a capture antibody to detect the c-myc-GST-IL8h recombinant protein, and the other one to detect the carcinoembryonic human protein (CEA). The sensitivity increased in both surfaces treated with tetrazine in comparison with the standard unmodified surface. The c-myc-GST-IL8h detection was around 10-fold more sensible on both tetrazine surfaces, while CEA ELISA detection increased 12-fold on surfaces coated with Tz-BSA. In conclusion, we show that it is possible to improve the ELISA sensitivity using this immobilization system, where capture antibodies bond covalently to surfaces.

scholarly journals Detection of platelet antibodies using a micro-enzyme-linked immunosorbent assay (ELISA)

Blood ◽  
1983 ◽  
Vol 61 (2) ◽  
pp. 311-317 ◽  
Author(s):  
CA Schiffer ◽  
V Young
Keyword(s):  
Immunosorbent Assay ◽  
Immune Thrombocytopenic Purpura ◽  
Hla Typing ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thrombocytopenic Purpura ◽  
Microtiter Plates ◽  
Platelet Antibodies ◽  
Liquid Suspension ◽  
Large Numbers ◽  
Specific Antigens

Abstract An enzyme-linked immunosorbent assay (ELISA) for the measurement of circulating platelet antibody and platelet-associated IgG (PAIgG) is described. The test is done in microtiter plates and rapidly provides quantitative and highly reproducible results. Alloantibodies from 28 of 30 multiple transfused patients and isoantibodies from 3 of 4 patients with immune thrombocytopenic purpura (ITP) were detected. PAIgG was elevated in all 4 patients with ITP, and HLA and platelet-specific antigens were reliably detected using HLA typing sera and anti-PIA1 antibody, respectively. Platelets preserved wither by dessication in the wells of the microtiter plates or in liquid suspension in saline at 4 degrees C gave results comparable to values using fresh platelets. Storage periods ranged from 30 days for dessicated platelets to more than 1 yr for platelets stored in suspension. The ability to utilize preserved platelets may allow relatively convenient screening of large numbers of potential platelet donors for alloimmunized patients.

Quantitative Determination of Dietary Lectin Activities by Enzyme-Linked Immunosorbent Assay Using Specific Glycoproteins Immobilized on Microtiter Plates

2002 ◽  
Vol 50 (22) ◽  
pp. 6266-6270 ◽  
Author(s):  
Simone Vincenzi ◽  
Gianni Zoccatelli ◽  
Fabio Perbellini ◽  
Corrado Rizzi ◽  
Roberto Chignola ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtiter Plates

Detection of platelet antibodies using a micro-enzyme-linked immunosorbent assay (ELISA)

Blood ◽  
1983 ◽  
Vol 61 (2) ◽  
pp. 311-317
Author(s):  
CA Schiffer ◽  
V Young
Keyword(s):  
Immunosorbent Assay ◽  
Immune Thrombocytopenic Purpura ◽  
Hla Typing ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thrombocytopenic Purpura ◽  
Microtiter Plates ◽  
Platelet Antibodies ◽  
Liquid Suspension ◽  
Large Numbers ◽  
Specific Antigens

An enzyme-linked immunosorbent assay (ELISA) for the measurement of circulating platelet antibody and platelet-associated IgG (PAIgG) is described. The test is done in microtiter plates and rapidly provides quantitative and highly reproducible results. Alloantibodies from 28 of 30 multiple transfused patients and isoantibodies from 3 of 4 patients with immune thrombocytopenic purpura (ITP) were detected. PAIgG was elevated in all 4 patients with ITP, and HLA and platelet-specific antigens were reliably detected using HLA typing sera and anti-PIA1 antibody, respectively. Platelets preserved wither by dessication in the wells of the microtiter plates or in liquid suspension in saline at 4 degrees C gave results comparable to values using fresh platelets. Storage periods ranged from 30 days for dessicated platelets to more than 1 yr for platelets stored in suspension. The ability to utilize preserved platelets may allow relatively convenient screening of large numbers of potential platelet donors for alloimmunized patients.

Enzyme-linked immunosorbent assay for free thyroxin in human serum.

1989 ◽  
Vol 35 (8) ◽  
pp. 1770-1772 ◽  
Author(s):  
S H Chui ◽  
K C Wan ◽  
C W Lam ◽  
W H Lewis
Keyword(s):  
Human Serum ◽  
Immunosorbent Assay ◽  
Turnaround Time ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtiter Plates ◽  
Coefficients Of Variation ◽  
Radioimmunoassay Method

Abstract We describe a simple enzyme-linked immunosorbent assay (ELISA) in which microtiter plates are used for determining the free thyroxin concentration (FT4) in serum. Only commercially available chemicals and reagents are needed, including the antiserum. The working range is 1 to 60 ng/L. Turnaround time is about 4 h. Within-run coefficients of variation (CV) for FT4 concentrations of 9.0, 19.0, and 35.0 ng/L were less than 5%; between-run CVs were 10% to 11%. Results by a radioimmunoassay method for 150 sera correlated well (r = 0.922).

Sign in / Sign up

Export Citation Format

Share Document