scholarly journals Effects of Melatonin and Pentoxyphilline Treatment on Newborn Rat Model of Hypoxic Ischemic Encephalopathy

2011 ◽  
Vol 70 ◽  
pp. 220-220
Author(s):  
O Soz ◽  
M K Turkmen ◽  
A Kumral ◽  
B Baykara ◽  
D C Yesilirmak ◽  
...  
Keyword(s):  
Rat Model ◽  
Hypoxic Ischemic Encephalopathy ◽  
Newborn Rat ◽  
Ischemic Encephalopathy

scholarly journals hUC-MSCs Exert a Neuroprotective Effect via Anti-apoptotic Mechanisms in a Neonatal HIE Rat Model

2019 ◽  
Vol 28 (12) ◽  
pp. 1552-1559 ◽  
Author(s):  
Jianwei Xu ◽  
Zhanhui Feng ◽  
Xianyao Wang ◽  
Ying Xiong ◽  
Lan Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Rat Model ◽  
Caspase 3 ◽  
Neuroprotective Effect ◽  
Hypoxic Ischemic Encephalopathy ◽  
Human Umbilical Cord ◽  
Motor Functions ◽  
Ischemic Encephalopathy

In this study, we investigated how human umbilical cord mesenchymal stem cells exerted a neuroprotective effect via antiapoptotic mechanisms in a neonatal hypoxic-ischemic encephalopathy rat model. A total of 78 10-day old (P10) rats were used. After human umbilical cord mesenchymal stem cells were collected from human umbilical cords and amplified in culture, they were administered to rat subjects 1 h after induced hypoxic-ischemic encephalopathy treatment. The short-term (48 h) and long-term (28 day) outcomes were evaluated after human umbilical cord mesenchymal stem cells treatment using neurobehavioral function assessment. Triphenyltetrazolium chloride monohydrate staining was performed at 48 h. Beclin-2 and caspase-3 levels were evaluated with Western blot and real time polymerase chain reaction at 48 h. Human umbilical cord mesenchymal stem cells were collected and administrated to hypoxic-ischemic encephalopathy pups by intracerebroventricular injection. Hypoxic-ischemic encephalopathy typically induced significant delay in development and caused impairment in both cognitive and motor functions in rat subjects. Human umbilical cord mesenchymal stem cells were shown to ameliorate hypoxic-ischemic encephalopathy-induced damage and improve both cognitive and motor functions. Although hypoxic-ischemic encephalopathy induced significant expression of caspase-3 and Beclin-2, human umbilical cord mesenchymal stem cells decreased the expression of both of them. Human umbilical cord mesenchymal stem cells may serve as a potential treatment to ameliorate brain injury in hypoxic-ischemic encephalopathy patients.

scholarly journals FASUDIL HYDROCHLORIDE EFFECTS ON EAAT2 AND ROCK2 PROTEIN EXPRESSIONS IN RAT MODEL OF NEONATAL HYPOXIC-ISCHEMIC ENCEPHALOPATHY.

2017 ◽  
Vol 5 (4) ◽  
pp. 1674-1679
Author(s):  
NdefiA ntimaYadiswaRobert ◽  
◽  
WangXian He ◽  
ZhangYa Li ◽  
ZhuZhang Long ◽  
...  
Keyword(s):  
Rat Model ◽  
Hypoxic Ischemic Encephalopathy ◽  
Protein Expressions ◽  
Ischemic Encephalopathy

Transplantation of multipotent astrocytic stem cells into a rat model of neonatal hypoxic–ischemic encephalopathy

2006 ◽  
Vol 1112 (1) ◽  
pp. 99-105 ◽  
Author(s):  
Tong Zheng ◽  
Candace Rossignol ◽  
Avital Leibovici ◽  
Kevin J. Anderson ◽  
Dennis A. Steindler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Rat Model ◽  
Hypoxic Ischemic Encephalopathy ◽  
Ischemic Encephalopathy

Bacteriophage carriers localize in the brain of a rat model of neonatal hypoxic‐ischemic encephalopathy

2021 ◽  
pp. 2100226
Author(s):  
Abdullah K. Alshememry ◽  
Jung‐Lynn Jonathan Yang ◽  
Edward A. Armstrong ◽  
Jerome Y. Yager ◽  
Larry D. Unsworth
Keyword(s):  
Rat Model ◽  
Hypoxic Ischemic Encephalopathy ◽  
The Brain ◽  
Ischemic Encephalopathy

Exogenous β-Hydroxybutyrate Treatment and Neuroprotection in a Suckling Rat Model of Hypoxic-Ischemic Encephalopathy

2018 ◽  
Vol 40 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Byong Sop Lee ◽  
Dong-Cheol Woo ◽  
Chul-Woong Woo ◽  
Ki-Soo Kim
Keyword(s):  
Rat Model ◽  
Hypoxic Ischemic Encephalopathy ◽  
Functional Tests ◽  
Rotarod Test ◽  
Object Recognition Test ◽  
Human Infants ◽  
Rat Pups ◽  
Cylinder Test ◽  
Neuroprotective Efficacy ◽  
Ischemic Encephalopathy

β-Hydroxybutyrate (BHB) is a representative ketone body that may play a role in the mitigation of neonatal hypoxic-ischemic encephalopathy by altering energy metabolism. This study aimed to investigate the neuroprotective efficacy of exogenous BHB administration in a suckling rat model after hypoxia-ischemia (HI). Thirteen-day-old (P13) rat pups were subjected to 120 min of hypoxia according to the Rice-Vannucci model. BHB (5.0 mmol/kg, HI-BHB) or vehicle (0.9% saline, HI-Veh) was administered 0, 2, 4, and 6 h after HI induction. Pathologic injury scores and the number of TUNEL-positive cells were evaluated on P15. Residual hemispheric volume was measured with T2-weighted MRI (on P27) and functional tests, such as the negative geotaxis test, rope suspension test, rotarod test, novel object recognition test, and cylinder test, were performed. Systemic ketosis (approx. 2.0–3.0 mM/L) was well tolerated by the rat pups with no difference in the mortality rate between both groups. Compared with the HI-Veh group, the HI-BHB group demonstrated significantly lower pathological scores as well as fewer TUNEL-positive cells. The intact residual hemispheric and hippocampal volumes were greater in the HI-BHB group than the HI-Veh group. However, the results of functional tests did not differ between both groups. Postischemic BHB administration reduced brain injury in suckling rats after HI. The safe clinical application of our animal model to human infants with HI requires further investigation.

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