scholarly journals Gene array analysis reveals a common Runx transcriptional programme controlling cell adhesion and survival

Oncogene ◽  
2008 ◽  
Vol 27 (44) ◽  
pp. 5856-5866 ◽  
Author(s):  
S Wotton ◽  
A Terry ◽  
A Kilbey ◽  
A Jenkins ◽  
P Herzyk ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Gene Array ◽  
Array Analysis ◽  
Gene Array Analysis

scholarly journals DICER1 and PRKRA in Colon Adenocarcinoma

2008 ◽  
Vol 3 ◽  
pp. BMI.S600 ◽  
Author(s):  
S. Chiosea ◽  
M. Acquafondata ◽  
J. Luo ◽  
SF. Kuan ◽  
RR. Seethala
Keyword(s):  
Rna Interference ◽  
Gene Array ◽  
Colon Adenocarcinoma ◽  
Clinicopathologic Features ◽  
Array Analysis ◽  
Gene Array Analysis ◽  
Microrna Profile ◽  
The Status ◽  
And Function ◽  
Dicer1 Expression

Differential microRNA expression in colon adenocarcinoma (CA) was previously reported. MicroRNA biogenesis and function requires a set of proteins designated as the microRNA machinery, which includes DICER1 and PRKRA. Loss of heterozygosity at 14q32.13 DICER1 locus was detected in up to 60% of CA cases. The in silico gene array analysis of CA showed down-regulation of DICER1 and an up-regulation of PRKRA. Immunohistochemically, DICER1 expression was abnormal in 65% of CA (95 of 147 cases). PRKRA was deregulated in 70% of CA (32 of 46 cases). Expression of DICER1 and PRKRA was correlated with clinicopathologic features of CA. DICER1 up-regulation was seen more commonly in women. Only 10 of 46 cases immunostained for both DICER1 and PRKRA showed normal levels of both DICER1 and PRKRA. Microsatellite status of 32 cases was determined. Microsatellite instable cases showed DICER1 up-regulation more commonly when compared to microsatellite stable cases; however, this trend was not statistically significant. Abnormal DICER1 and/or PRKRA expression might explain the observed changes in microRNA profile. The status of the endogenous DICER1 and PRKRA in CA may help to predict the response to future RNA interference-based therapy.

Stability of gene expression in postmortem brain revealed by cDNA gene array analysis

2002 ◽  
Vol 942 (1-2) ◽  
pp. 120-123 ◽  
Author(s):  
Stacey A. Trotter ◽  
Louis B. Brill II ◽  
James P. Bennett
Keyword(s):  
Gene Expression ◽  
Gene Array ◽  
Postmortem Brain ◽  
Array Analysis ◽  
Gene Array Analysis

Gene array analysis of the expression profile associated with intestinal cell differentiation

2001 ◽  
Vol 120 (5) ◽  
pp. A88
Author(s):  
Qingding Wang ◽  
Robert Thomas ◽  
Nan Li ◽  
Xiaofu Wang ◽  
B. Mark Evers
Keyword(s):  
Cell Differentiation ◽  
Expression Profile ◽  
Gene Array ◽  
Intestinal Cell ◽  
Array Analysis ◽  
Gene Array Analysis

Effect of RTVP-1 on the mesenchymal transformation of glioma stem cells (GSCs) and their self-renewal and migration.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. 2073-2073
Author(s):  
Chaya Brodie ◽  
Nissim Giladi ◽  
Amotz Ziv-Av ◽  
Hae Kyung Lee ◽  
Susan Finniss ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Array ◽  
Tumor Grade ◽  
Small Population ◽  
Array Analysis ◽  
Self Renewal ◽  
Human Neural Stem Cells ◽  
Gene Array Analysis ◽  
And Migration ◽  
Mesenchymal Transformation

2073 Background: Glioblastoma (GBM), the most aggressive primary brain tumors, exhibit increased invasiveness and resistance to anti-tumor treatments. GBM are categorized into proneural, neural, classical and mesenchymal subgroups, the latter being characterized by increased invasion and poor prognosis. We recently reported that RTVP-1 is highly expressed in GBM and its expression is correlated with astrocytic tumor grade. Methods: We employed promoter and Chip analyses, analysis of tumor specimens submitted to TCGA , GSC self-renewal and migration assays, mesenchymal and neural differentiation, gene array analysis and pull-down assay followed by FRET. Results: The RTVP-1 promoter binds STAT3 and C/EBPb, the transcription factors that regulate mesenchymal transformation of GBM. The expression of RTVP-1 is higher in mesenchymal GBM and is inversely correlated with patient survival in the proneural group. We examined the expression and functions of RTVP-1 in GSCs, a small population of cancer stem cells that are implicated in the increased migration, radio- and chemo-resistance of GSCs and tumor recurrence. RTVP-1 was expressed in the different GSCs but not in the normal human neural stem cells (NSCs). Overexpression of RTVP-1 in NSCs induced their mesenchymal transformation, whereas silencing of RTVP-1 in GSCs decreased their mesenchymal and increased their neural phenotypes. Moreover, RTVP-1 promoted the self-renewal and migration of GSCs. Using gene array analysis of RTVP-1 silenced cells we identified IL-6 and CXCR4 as major mediators of RTVP-1 effects on the mesenchymal transformation and self-renewal of GSCs. In addition, using a pull down assay with His-tagged RTVP-1 we identified N-WASP and hnRNPK as novel interacting proteins of RTVP-1 that mediate its effects on glioma cell migration. Conclusions: RTVP-1 expression is associated with mesenchymal transformation of GSCs. RTVP-1 promotes self-renewal and migration of GSCs and these effects are mediated by the increased expression of IL-6 and CXCR4 and via interaction with N-WASP and hnRNPK. Collectively, these results suggest that RTVP-1 may represent a novel diagnostic marker and a therapeutic target in GBM.

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