scholarly journals Reciprocal Hemizygosity Analysis of Mouse Hepatic Lipase Reveals Influence on Obesity

2004 ◽  
Vol 12 (2) ◽  
pp. 292-305 ◽  
Author(s):  
Poupak Farahani ◽  
Janis S. Fisler ◽  
Howard Wong ◽  
Adam L. Diament ◽  
Nengjun Yi ◽  
...  
Keyword(s):  
Hepatic Lipase ◽  
Reciprocal Hemizygosity Analysis

scholarly journals Reciprocal hemizygosity analysis reveals that the Saccharomyces cerevisiae CGI121 gene affects lag time duration in synthetic grape must

2021 ◽  
Author(s):  
Runze Li ◽  
Rebecca C Deed
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Temperatures ◽  
Lag Time ◽  
Lag Phase ◽  
Phenotypic Traits ◽  
Time Duration ◽  
Phase Duration ◽  
Grape Must ◽  
The Impact ◽  
Reciprocal Hemizygosity Analysis

Abstract It is standard practice to ferment white wines at low temperatures (10-18 °C). However, low temperatures increase fermentation duration and risk of problem ferments, leading to significant costs. The lag duration at fermentation initiation is heavily impacted by temperature; therefore, identification of Saccharomyces cerevisiae genes influencing fermentation kinetics is of interest for winemaking. We selected 28 S. cerevisiae BY4743 single deletants, from a prior list of open reading frames (ORFs) mapped to quantitative trait loci (QTLs) on chromosomes VII and XIII, influencing the duration of fermentative lag time. Five BY4743 deletants, Δapt1, Δcgi121, Δclb6, Δrps17a, and Δvma21, differed significantly in their fermentative lag duration compared to BY4743 in synthetic grape must (SGM) at 15 °C, over 72 h. Fermentation at 12.5 °C for 528 h confirmed the longer lag times of BY4743 Δcgi121, Δrps17a, and Δvma21. These three candidate ORFs were deleted in S. cerevisiae RM11-1a and S288C to perform single reciprocal hemizygosity analysis (RHA). RHA hybrids and single deletants of RM11-1a and S288C were fermented at 12.5 °C in SGM and lag time measurements confirmed that the S288C allele of CGI121 on chromosome XIII, encoding a component of the EKC/KEOPS complex, increased fermentative lag phase duration. Nucleotide sequences of RM11-1a and S288C CGI121 alleles differed by only one synonymous nucleotide, suggesting that intron splicing, codon bias, or positional effects might be responsible for the impact on lag phase duration. This research demonstrates a new role of CGI121 and highlights the applicability of QTL analysis for investigating complex phenotypic traits in yeast.

scholarly journals Hepatic lipase facilitates the selective uptake of cholesteryl esters from remnant lipoproteins in apoE-deficient mice

1998 ◽  
Vol 39 (12) ◽  
pp. 2436-2442 ◽  
Author(s):  
Marcelo J.A. Amar ◽  
Klaus A. Dugi ◽  
Changting C. Haudenschild ◽  
Robert D. Shamburek ◽  
Bernhard Foger ◽  
...  
Keyword(s):  
Hepatic Lipase ◽  
Cholesteryl Esters ◽  
Selective Uptake ◽  
Remnant Lipoproteins ◽  
Deficient Mice

scholarly journals Metabolism of lipoproteins containing apolipoprotein B in hepatic lipase-deficient mice

1998 ◽  
Vol 39 (8) ◽  
pp. 1661-1668 ◽  
Author(s):  
Shiqiang Qiu ◽  
Nathalie Bergeron ◽  
Leila Kotite ◽  
Ronald M. Krauss ◽  
Andre Bensadoun ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Hepatic Lipase ◽  
Deficient Mice

scholarly journals Estrogen receptor-mediated repression of human hepatic lipase gene transcription

2002 ◽  
Vol 43 (3) ◽  
pp. 383-391 ◽  
Author(s):  
Daniel R. Jones ◽  
Robert J. Schmidt ◽  
Richard T. Pickard ◽  
Patricia S. Foxworthy ◽  
Patrick I. Eacho
Keyword(s):  
Estrogen Receptor ◽  
Gene Transcription ◽  
Hepatic Lipase ◽  
Lipase Gene ◽  
Hepatic Lipase Gene
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