scholarly journals Role of Insulin and Free Fatty Acids in the Regulation ofobGene Expression and Plasma Leptin in Normal Rats

2004 ◽  
Vol 12 (12) ◽  
pp. 2062-2069 ◽  
Author(s):  
Claudio Pagano ◽  
Alberto Dorigo ◽  
Enzo Nisoli ◽  
Cristina Tonello ◽  
Alessandra Calcagno ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Plasma Leptin

Alcohol intake impairs glucose counterregulation during acute insulin-induced hypoglycemia in IDDM patients. Evidence for a critical role of free fatty acids

Diabetes ◽  
1993 ◽  
Vol 42 (11) ◽  
pp. 1626-1634 ◽  
Author(s):  
A. Avogaro ◽  
P. Beltramello ◽  
L. Gnudi ◽  
A. Maran ◽  
A. Valerio ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Alcohol Intake ◽  
Critical Role

scholarly journals Free Fatty acids receptors (FFAR2 and FFAR3) control cell proliferation by regulating cellular glucose uptake

2019 ◽  
Author(s):  
Mohammad Aziz ◽  
Saeed Al Mahri ◽  
Amal Alghamdi ◽  
Maaged AlAkiel ◽  
Monira Al Aujan ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Cell Proliferation ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Glucose Uptake ◽  
Microarray Data ◽  
Free Fatty Acid Receptors

Abstract Background Colorectal cancer is a worldwide problem which has been associated with changes in diet and lifestyle pattern. As a result of colonic fermentation of dietary fibres, short chain free fatty acids are generated which activate Free Fatty Acid Receptors 2 and 3 (FFAR2 and FFAR3). FFAR2 and FFAR3 genes are abundantly expressed in colonic epithelium and play an important role in the metabolic homeostasis of colonic epithelial cells. Earlier studies point to the involvement of FFAR2 in colorectal carcinogenesis. Methods Transcriptome analysis console was used to analyse microarray data from patients and cell lines. We employed shRNA mediated down regulation of FFAR2 and FFAR3 genes which was assessed using qRT-PCR. Assays for glucose uptake and cAMP generation was done along with immunofluorescence studies. For measuring cell proliferation, we employed real time electrical impedance based assay available from xCelligence. Results Microarray data analysis of colorectal cancer patient samples showed a significant down regulation of FFAR2 gene expression. This prompted us to study the FFAR2 in colorectal cancer. Since, FFAR3 shares significant structural and functional homology with FFAR2, we knocked down both these receptors in colorectal cancer cell line HCT 116. These modified cell lines exhibited higher proliferation rate and were found to have increased glucose uptake as well as increased level of GLUT1. Since, FFAR2 and FFAR3 signal through G protein subunit (Gαi), knockdown of these receptors was associated with increased cAMP. Inhibition of PKA did not alter the growth and proliferation of these cells indicating a mechanism independent of cAMP/PKA pathway. Conclusion: Our results suggest role of FFAR2/FFAR3 genes in increased proliferation of colon cancer cells via enhanced glucose uptake and exclude the role of protein kinase A mediated cAMP signalling. Alternate pathways could be involved that would ultimately result in increased cell proliferation as a result of down regulated FFAR2/FFAR3 genes. This study paves the way to understand the mechanism of action of short chain free fatty acid receptors in colorectal cancer.

Does valproate therapy in epileptic patients contribute to changing atherosclerosis risk factors? The role of lipids and free fatty acids

2016 ◽  
Vol 68 (6) ◽  
pp. 1339-1344 ◽  
Author(s):  
Elżbieta Płonka-Półtorak ◽  
Paweł Zagrodzki ◽  
Jadwiga Kryczyk-Kozioł ◽  
Tuomas Westermarck ◽  
Pekka Kaipainen ◽  
...  
Keyword(s):  
Risk Factors ◽  
Fatty Acids ◽  
Free Fatty Acids ◽  
Atherosclerosis Risk ◽  
Epileptic Patients ◽  
Atherosclerosis Risk Factors

The antihyperglycemic effect of curcumin in high fat diet fed rats. Role of TNF-α and free fatty acids

2011 ◽  
Vol 49 (5) ◽  
pp. 1129-1140 ◽  
Author(s):  
Mohamed A. El-Moselhy ◽  
Ashraf Taye ◽  
Sara Shaaban Sharkawi ◽  
Suzan F.I. El-Sisi ◽  
Ahmed Fahmy Ahmed
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
High Fat Diet ◽  
High Fat ◽  
Antihyperglycemic Effect ◽  
Tnf Α

scholarly journals The nicotinic acid analogue acipimox increases plasma leptin and decreases free fatty acids in type 2 diabetic patients

2000 ◽  
pp. 389-395 ◽  
Author(s):  
D Worm ◽  
J Vinten ◽  
A Vaag ◽  
JE Henriksen ◽  
H Beck-Nielsen
Keyword(s):  
Fatty Acids ◽  
Nicotinic Acid ◽  
Free Fatty Acids ◽  
Experimental Period ◽  
Mirror Image ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Double Blind ◽  
Plasma Leptin

The effect of 3 days of intensive treatment with acipimox, an antilipolytic nicotinic acid derivative, on plasma leptin levels was studied in eight patients with Type 2 diabetes mellitus in a double-blind, placebo-controlled, cross-over study. Acipimox reduced plasma free fatty acids (FFA) markedly and lowered plasma triglycerides, glucose and insulin. Plasma leptin levels were elevated in all eight patients during 3 days of acipimox treatment (mean increase+/-s.e.: 2.38+/-0.57ng/ml, P<0.005) and the 24h mean effect of acipimox on leptin levels increased during the experimental period (P<0.03). The effect on plasma insulin and glucose resembled a mirror image of the effect on plasma leptin during 3 days of treatment. The suggestion that leptin mediates insulin resistance and may be involved in the development of the diabetic syndrome cannot be supported by the present results. It has been reported that FFA stimulates leptin secretion. Surprisingly, despite a markedly reduced FFA level, leptin concentration increased in the present study. We suggest that a primary acipimox effect is to increase leptin secretion, and that this prevails over the reduced FFA stimulus.

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