A newly discovered function for RNase L in regulating translation termination

Florence Le Roy; Tamim Salehzada; Catherine Bisbal; Joseph P Dougherty; Stuart W Peltz

doi:10.1038/nsmb944

A newly discovered function for RNase L in regulating translation termination

Nature Structural & Molecular Biology ◽

10.1038/nsmb944 ◽

2005 ◽

Vol 12 (6) ◽

pp. 505-512 ◽

Cited By ~ 53

Author(s):

Florence Le Roy ◽

Tamim Salehzada ◽

Catherine Bisbal ◽

Joseph P Dougherty ◽

Stuart W Peltz

Keyword(s):

Translation Termination ◽

Rnase L

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The iron–sulphur protein RNase L inhibitor functions in translation termination

EMBO Reports ◽

10.1038/embor.2009.272 ◽

2010 ◽

Vol 11 (3) ◽

pp. 214-219 ◽

Cited By ~ 80

Author(s):

Sohail Khoshnevis ◽

Thomas Gross ◽

Carmen Rotte ◽

Claudia Baierlein ◽

Ralf Ficner ◽

...

Keyword(s):

Translation Termination ◽

Rnase L ◽

Rnase L Inhibitor

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Influence of nascent polypeptide positive charges on translation dynamics

Biochemical Journal ◽

10.1042/bcj20200303 ◽

2020 ◽

Vol 477 (15) ◽

pp. 2921-2934

Author(s):

Rodrigo D. Requião ◽

Géssica C. Barros ◽

Tatiana Domitrovic ◽

Fernando L. Palhano

Keyword(s):

Mrna Decay ◽

Translation Termination ◽

Published Data ◽

Translation Efficiency ◽

Amino Acid Residues ◽

High Concentration ◽

Strong Argument ◽

Translation Arrest ◽

Exit Tunnel ◽

Positively Charged

Protein segments with a high concentration of positively charged amino acid residues are often used in reporter constructs designed to activate ribosomal mRNA/protein decay pathways, such as those involving nonstop mRNA decay (NSD), no-go mRNA decay (NGD) and the ribosome quality control (RQC) complex. It has been proposed that the electrostatic interaction of the positively charged nascent peptide with the negatively charged ribosomal exit tunnel leads to translation arrest. When stalled long enough, the translation process is terminated with the degradation of the transcript and an incomplete protein. Although early experiments made a strong argument for this mechanism, other features associated with positively charged reporters, such as codon bias and mRNA and protein structure, have emerged as potent inducers of ribosome stalling. We carefully reviewed the published data on the protein and mRNA expression of artificial constructs with diverse compositions as assessed in different organisms. We concluded that, although polybasic sequences generally lead to lower translation efficiency, it appears that an aggravating factor, such as a nonoptimal codon composition, is necessary to cause translation termination events.

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