PML bodies control the nuclear dynamics and function of the CHFR mitotic checkpoint protein

2004 ◽  
Vol 11 (11) ◽  
pp. 1114-1121 ◽  
Author(s):  
Matthew J Daniels ◽  
Alexander Marson ◽  
Ashok R Venkitaraman
Keyword(s):  
Mitotic Checkpoint ◽  
Nuclear Dynamics ◽  
Checkpoint Protein ◽  
Pml Bodies ◽  
Dynamics And Function ◽  
And Function ◽  
Mitotic Checkpoint Protein

scholarly journals Requirement of the Dynein-adaptor Spindly for mitotic and post-mitotic functions in Drosophila

2017 ◽  
Author(s):  
Giuliana D. Clemente ◽  
Matthew R. Hannaford ◽  
Jens Januschke ◽  
Eric R. Griffis ◽  
Hans-Arno J. Muller
Keyword(s):  
Germ Line ◽  
Mitotic Checkpoint ◽  
Checkpoint Protein ◽  
C Elegans ◽  
Protein Levels ◽  
Egg Morphology ◽  
Female Germ Line ◽  
And Function ◽  
Mitotic Checkpoint Protein ◽  
Dynactin Complex

AbstractSpindly is a mitotic checkpoint protein originally identified as a specific regulator of Dynein activity at the kinetochore. In metaphase, Spindly recruits the Dynein/Dynactin complex, promoting the establishment of stable kinetochore-microtubule interactions and progression into anaphase. While details of Spindly function in mitosis have been worked out in cultured human cells and in the C. elegans zygote, the function of Spindly within the context of an organism has not yet been addressed. Here we present loss- and gain-of-function studies of Spindly in Drosophila. We investigated the requirements of distinct protein domains for the localisation and function of Spindly. We find that knock-down of Spindly results in a range of mitotic defects in the female germ line and during cleavage divisions in embryogenesis. Overexpression of Spindly in the female germ line is embryonic lethal and results in altered egg morphology. To determine whether Spindly plays a role in post-mitotic cells we altered Spindly protein levels in migrating cells and found that ovarian border cell migration is sensitive to the levels of Spindly protein. Our study uncovers novel functions of the mitotic checkpoint protein Spindly in Drosophila.

scholarly journals Bub1 mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis

2007 ◽  
Vol 179 (2) ◽  
pp. 255-267 ◽  
Author(s):  
Karthik Jeganathan ◽  
Liviu Malureanu ◽  
Darren J. Baker ◽  
Susan C. Abraham ◽  
Jan M. van Deursen
Keyword(s):  
Cell Death ◽  
Chromosome Segregation ◽  
Physiological Role ◽  
Mitotic Checkpoint ◽  
Critical Threshold ◽  
Wild Type ◽  
Checkpoint Protein ◽  
Chromosome Missegregation ◽  
Mitotic Checkpoint Protein

The physiological role of the mitotic checkpoint protein Bub1 is unknown. To study this role, we generated a series of mutant mice with a gradient of reduced Bub1 expression using wild-type, hypomorphic, and knockout alleles. Bub1 hypomorphic mice are viable, fertile, and overtly normal despite weakened mitotic checkpoint activity and high percentages of aneuploid cells. Bub1 haploinsufficient mice, which have a milder reduction in Bub1 protein than Bub1 hypomorphic mice, also exhibit reduced checkpoint activity and increased aneuploidy, but to a lesser extent. Although cells from Bub1 hypomorphic and haploinsufficient mice have similar rates of chromosome missegregation, cell death after an aberrant separation decreases dramatically with declining Bub1 levels. Importantly, Bub1 hypomorphic mice are highly susceptible to spontaneous tumors, whereas Bub1 haploinsufficient mice are not. These findings demonstrate that loss of Bub1 below a critical threshold drives spontaneous tumorigenesis and suggest that in addition to ensuring proper chromosome segregation, Bub1 is important for mediating cell death when chromosomes missegregate.

scholarly journals The Vertebrate Mitotic Checkpoint Protein BUBR1 Is an Unusual Pseudokinase

2012 ◽  
Vol 22 (6) ◽  
pp. 1321-1329 ◽  
Author(s):  
Saskia J.E. Suijkerbuijk ◽  
Teunis J.P. van Dam ◽  
G. Elif Karagöz ◽  
Eleonore von Castelmur ◽  
Nina C. Hubner ◽  
...  
Keyword(s):  
Mitotic Checkpoint ◽  
Checkpoint Protein ◽  
Mitotic Checkpoint Protein

scholarly journals PML3 Orchestrates the Nuclear Dynamics and Function of TIP60

2009 ◽  
Vol 284 (13) ◽  
pp. 8747-8759 ◽  
Author(s):  
Quan Wu ◽  
Haixiang Hu ◽  
Jianping Lan ◽  
Chibuzo Emenari ◽  
Zhiyong Wang ◽  
...  
Keyword(s):  
Nuclear Dynamics ◽  
Dynamics And Function ◽  
And Function

scholarly journals Altered Expression of the Early Mitotic Checkpoint Protein, CHFR, in Breast Cancers: Implications for Tumor Suppression

2007 ◽  
Vol 67 (13) ◽  
pp. 6064-6074 ◽  
Author(s):  
Lisa M. Privette ◽  
Maria E. González ◽  
Lei Ding ◽  
Celina G. Kleer ◽  
Elizabeth M. Petty
Keyword(s):  
Tumor Suppression ◽  
Mitotic Checkpoint ◽  
Breast Cancers ◽  
Altered Expression ◽  
Checkpoint Protein ◽  
Mitotic Checkpoint Protein
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