GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop

2007 ◽  
Vol 14 (4) ◽  
pp. 280-286 ◽  
Author(s):  
Gustavo Fenalti ◽  
Ruby H P Law ◽  
Ashley M Buckle ◽  
Christopher Langendorf ◽  
Kellie Tuck ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase ◽  
Gaba Production ◽  
Catalytic Loop

scholarly journals Structural characterization of the mechanism through which human glutamic acid decarboxylase auto-activates

2013 ◽  
Vol 33 (1) ◽  
Author(s):  
Christopher G. Langendorf ◽  
Kellie L. Tuck ◽  
Trevor L. G. Key ◽  
Gustavo Fenalti ◽  
Robert N. Pike ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Enzyme Inactivation ◽  
Aminobutyric Acid ◽  
Acid Decarboxylase ◽  
X Ray ◽  
Terminal Domain ◽  
Catalytic Loop ◽  
Structural Insights

Imbalances in GABA (γ-aminobutyric acid) homoeostasis underlie psychiatric and movement disorders. The ability of the 65 kDa isoform of GAD (glutamic acid decarboxylase), GAD65, to control synaptic GABA levels is influenced through its capacity to auto-inactivate. In contrast, the GAD67 isoform is constitutively active. Previous structural insights suggest that flexibility in the GAD65 catalytic loop drives enzyme inactivation. To test this idea, we constructed a panel of GAD65/67 chimaeras and compared the ability of these molecules to auto-inactivate. Together, our data reveal the important finding that the C-terminal domain of GAD plays a key role in controlling GAD65 auto-inactivation. In support of these findings, we determined the X-ray crystal structure of a GAD65/67 chimaera that reveals that the conformation of the catalytic loop is intimately linked to the C-terminal domain.

Autoantibodies in IDDM primarily recognize the 65,000-M(r) rather than the 67,000-M(r) isoform of glutamic acid decarboxylase

Diabetes ◽  
1993 ◽  
Vol 42 (4) ◽  
pp. 631-636 ◽  
Author(s):  
W. A. Hagopian ◽  
B. Michelsen ◽  
A. E. Karlsen ◽  
F. Larsen ◽  
A. Moody ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Acid Decarboxylase

Brain FDG‐PET findings in glutamic acid decarboxylase antibody‐associated epilepsy

2021 ◽  
Author(s):  
Neus Mongay‐Ochoa ◽  
Jacint Sala‐Padró ◽  
Gabriel Reynés‐Llompart ◽  
Laura Rodríguez‐Bel ◽  
Sònia Jaraba ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Fdg Pet ◽  
Acid Decarboxylase ◽  
Glutamic Acid Decarboxylase Antibody
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