Spliceosome assembly is coupled to RNA polymerase II dynamics at the 3′ end of human genes

2011 ◽  
Vol 18 (10) ◽  
pp. 1115-1123 ◽  
Author(s):  
Sandra Bento Martins ◽  
José Rino ◽  
Teresa Carvalho ◽  
Célia Carvalho ◽  
Minoru Yoshida ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Spliceosome Assembly ◽  
Human Genes

scholarly journals A Human RNA Polymerase II-containing Complex Associated with Factors Necessary for Spliceosome Assembly

2001 ◽  
Vol 277 (11) ◽  
pp. 9302-9306 ◽  
Author(s):  
François Robert ◽  
Marco Blanchette ◽  
Olivier Maes ◽  
Benoit Chabot ◽  
Benoit Coulombe
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Spliceosome Assembly

scholarly journals Human RNA polymerase II subunit hsRPB7 functions in yeast and influences stress survival and cell morphology.

1995 ◽  
Vol 6 (7) ◽  
pp. 759-775 ◽  
Author(s):  
V Khazak ◽  
P P Sadhale ◽  
N A Woychik ◽  
R Brent ◽  
E A Golemis
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Specific Pattern ◽  
Yeast Cells ◽  
Human Genes ◽  
Complete Set ◽  
High And Low Temperatures ◽  
And Function ◽  
Two Hybrid ◽  
Potential Regulatory Role

Using a screen to identify human genes that promote pseudohyphal conversion in Saccharomyces cerevisiae, we obtained a cDNA encoding hsRPB7, a human homologue of the seventh largest subunit of yeast RNA polymerase II (RPB7). Overexpression of yeast RPB7 in a comparable strain background caused more pronounced cell elongation than overexpression of hsRPB7. hsRPB7 sequence and function are strongly conserved with its yeast counterpart because its expression can rescue deletion of the essential RPB7 gene at moderate temperatures. Further, immuno-precipitation of RNA polymerase II from yeast cells containing hsRPB7 revealed that the hsRPB7 assembles the complete set of 11 other yeast subunits. However, at temperature extremes and during maintenance at stationary phase, hsRPB7-containing yeast cells lose viability rapidly, stress-sensitive phenotypes reminiscent of those associated with deletion of the RPB4 subunit with which RPB7 normally complexes. Two-hybrid analysis revealed that although hsRPB7 and RPB4 interact, the association is of lower affinity than the RPB4-RPB7 interaction, providing a probable mechanism for the failure of hsRPB7 to fully function in yeast cells at high and low temperatures. Finally, surprisingly, hsRPB7 RNA in human cells is expressed in a tissue-specific pattern that differs from that of the RNA polymerase II largest subunit, implying a potential regulatory role for hsRPB7. Taken together, these results suggest that some RPB7 functions may be analogous to those possessed by the stress-specific prokaryotic sigma factor rpoS.

scholarly journals Functional Association of Gdown1 with RNA Polymerase II Poised on Human Genes

2012 ◽  
Vol 45 (1) ◽  
pp. 38-50 ◽  
Author(s):  
Bo Cheng ◽  
Tiandao Li ◽  
Peter B. Rahl ◽  
Todd E. Adamson ◽  
Nicholas B. Loudas ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Functional Association ◽  
Human Genes

Nutrient-regulated gene expression in eukaryotes

2006 ◽  
Vol 73 ◽  
pp. 85-96 ◽  
Author(s):  
Richard J. Reece ◽  
Laila Beynon ◽  
Stacey Holden ◽  
Amanda D. Hughes ◽  
Karine Rébora ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcriptional Control ◽  
Direct Interaction ◽  
Signalling Pathways ◽  
A Cell ◽  
One Step ◽  
Higher Eukaryotes ◽  
Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

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