scholarly journals α-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel

2010 ◽  
Vol 17 (3) ◽  
pp. 313-317 ◽  
Author(s):  
Shashi Bhushan ◽  
Marco Gartmann ◽  
Mario Halic ◽  
Jean-Paul Armache ◽  
Alexander Jarasch ◽  
...  
Keyword(s):  
Nascent Polypeptide ◽  
Exit Tunnel ◽  
Polypeptide Chains

O-GlcNAc occurs cotranslationally to stabilize nascent polypeptide chains

2015 ◽  
Vol 11 (5) ◽  
pp. 319-325 ◽  
Author(s):  
Yanping Zhu ◽  
Ta-Wei Liu ◽  
Samy Cecioni ◽  
Razieh Eskandari ◽  
Wesley F Zandberg ◽  
...  
Keyword(s):  
Nascent Polypeptide ◽  
Polypeptide Chains

scholarly journals Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuzuru Itoh ◽  
Andreas Naschberger ◽  
Narges Mortezaei ◽  
Johannes M. Herrmann ◽  
Alexey Amunts
Keyword(s):  
Large Subunit ◽  
Small Subunit ◽  
Translation Regulation ◽  
Structural Rearrangements ◽  
Nascent Polypeptide ◽  
Maturation Factor ◽  
Exit Tunnel ◽  
Assembly Intermediate ◽  
Insight Into ◽  
Inhibitory Factor 1

Abstract Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF1) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.

Folding of nascent polypeptide chains in a high molecular mass assembly with molecular chaperones

Nature ◽  
1994 ◽  
Vol 370 (6485) ◽  
pp. 111-117 ◽  
Author(s):  
Judith Frydman ◽  
Elmar Nimmesgern ◽  
Kenzo Ohtsuka ◽  
F. Ulrich Hartl
Keyword(s):  
Molecular Mass ◽  
Molecular Chaperones ◽  
High Molecular Mass ◽  
Nascent Polypeptide ◽  
Mass Assembly ◽  
Polypeptide Chains
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