Ultrafast enzymatic reaction dynamics in protochlorophyllide oxidoreductase

2003 ◽  
Vol 10 (6) ◽  
pp. 491-492 ◽  
Author(s):  
Derren J Heyes ◽  
C Neil Hunter ◽  
Ivo H M van Stokkum ◽  
Rienk van Grondelle ◽  
Marie Louise Groot
Keyword(s):  
Enzymatic Reaction ◽  
Reaction Dynamics ◽  
Protochlorophyllide Oxidoreductase

scholarly journals Phase separation of chromatin brush driven by enzymatic reaction dynamics of histone posttranslational modifications

2020 ◽  
Author(s):  
Tetsuya Yamamoto ◽  
Takahiro Sakaue ◽  
Helmut Schiessel
Keyword(s):  
Phase Separation ◽  
Posttranslational Modifications ◽  
Enzymatic Reaction ◽  
Reaction Dynamics ◽  
The State ◽  
Condensed Chromatin ◽  
Enzymatic Reactions ◽  
Histone Tails ◽  
Undifferentiated Cells ◽  
Brush Height

The nuclei of undifferentiated cells show uniform decompacted chromatin while during development nuclei decrease in size and foci of condensed chromatin appear, reminiscent of phase separation. This study is motivated by recent experiments that suggest that the unbinding of enzymes that chemically modify (acetylate) histone tails causes decompaction of condensed chromatin. Here we take into account the enzymatic reactions of histone modifications to predict the phase separation of chromatin in a model system, the chromatin brush, which mimics chromatin at the proximity of a nuclear membrane. The model contains ‘activators’ and ‘silencers’, which change the state of the nucleosomes to (transcriptionally) active or inactive via the Michaelis-Menten kinetics. Our theory predicts that the chromatin brush will phase separate when the brush height is reduced below a threshold height. The phase separation is driven by an anti-correlation: Activators change the state of nucleosomes to the active state suppressing the binding of silencers to these nucleosomes and vice versa.

Light-Driven Enzymatic Reaction in Thermophilic Protochlorophyllide Oxidoreductase

2006 ◽  
pp. 428-431
Author(s):  
O. Sytina ◽  
M.L. Groot ◽  
R. van Grondelle ◽  
D.J. Heyes ◽  
C.N. Hunter
Keyword(s):  
Enzymatic Reaction ◽  
Protochlorophyllide Oxidoreductase

Reaction Dynamics — Recent Advances

1991 ◽  
Vol 174 (Part_2) ◽  
pp. 225-225
Author(s):  
Wolfgang Schirmer
Keyword(s):  
Reaction Dynamics ◽  
Recent Advances

Computer Simulation of Amperometric Biosensor Response to Mixtures of Compounds

2002 ◽  
Vol 7 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
J. Christensen ◽  
F. Ivanauskas ◽  
J. Kulys
Keyword(s):  
Computer Simulation ◽  
Enzymatic Reaction ◽  
Diffusion Equations ◽  
Response Data ◽  
Finite Difference Technique ◽  
Stationary Diffusion ◽  
Biosensor Array ◽  
Menten Kinetic ◽  
Biosensor Response

A mathematical model of amperometric biosensors has been developed. The model bases on non-stationary diffusion equations containing a non-linear term related to Michaelis-Menten kinetic of the enzymatic reaction. The model describes the biosensor response to mixtures of multiple compounds in two regimes of analysis: batch and flow injection. Using computer simulation, large amount of biosensor response data were synthesised for calibration of a biosensor array to be used for characterization of wastewater. The computer simulation was carried out using the finite difference technique.

Sign in / Sign up

Export Citation Format

Share Document