A HEX-1 crystal lattice required for Woronin body function in Neurospora crassa

2003 ◽  
Vol 10 (4) ◽  
pp. 264-270 ◽  
Author(s):  
Ping Yuan ◽  
Gregory Jedd ◽  
Desigan Kumaran ◽  
Subramanyam Swaminathan ◽  
Helen Shio ◽  
...  
Keyword(s):  
Crystal Lattice ◽  
Neurospora Crassa ◽  
Body Function ◽  
Woronin Body

scholarly journals Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting

2008 ◽  
Vol 180 (2) ◽  
pp. 325-339 ◽  
Author(s):  
Fangfang Liu ◽  
Seng Kah Ng ◽  
Yanfen Lu ◽  
Wilson Low ◽  
Julian Lai ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Neurospora Crassa ◽  
Filamentous Fungi ◽  
Dense Core ◽  
Cell Cortex ◽  
Protein Matrix ◽  
Woronin Body ◽  
Membrane Bound ◽  
And Control ◽  
Dual Functions

Woronin bodies (WBs) are dense-core organelles that are found exclusively in filamentous fungi and that seal the septal pore in response to wounding. These organelles consist of a membrane-bound protein matrix comprised of the HEX protein and, although they form from peroxisomes, their biogenesis is poorly understood. In Neurospora crassa, we identify Woronin sorting complex (WSC), a PMP22/MPV17-related membrane protein with dual functions in WB biogenesis. WSC localizes to large peroxisome membranes where it self-assembles into detergent-resistant oligomers that envelop HEX assemblies, producing asymmetrical nascent WBs. In a reaction requiring WSC, these structures are delivered to the cell cortex, which permits partitioning of the nascent WB and WB inheritance. Our findings suggest that WSC and HEX collaborate and control distinct aspects of WB biogenesis and that cortical association depends on WSC, which in turn depends on HEX. This dependency helps order events across the organellar membrane, permitting the peroxisome to produce a second organelle with a distinct composition and intracellular distribution.

scholarly journals SO, a Protein Involved in Hyphal Fusion in Neurospora crassa, Localizes to Septal Plugs

2006 ◽  
Vol 6 (1) ◽  
pp. 84-94 ◽  
Author(s):  
André Fleiβner ◽  
N. Louise Glass
Keyword(s):  
Cell Death ◽  
Neurospora Crassa ◽  
Colony Development ◽  
Independent Manner ◽  
Hyphal Fusion ◽  
Filamentous Ascomycete ◽  
Sealing Material ◽  
Woronin Body ◽  
Local Injury ◽  
Extensive Damage

ABSTRACT The colony of a filamentous ascomycete fungus typically grows as a multinucleate syncytium. While this syncytial organization has developmental advantages, it bears the risk of extensive damage caused by local injury of hyphae. Loss of cytoplasm in injured hyphae is restricted by the fast and efficient sealing of the central pores of hyphal crosswalls, or septa, by a peroxisome-derived organelle called the Woronin body. The formation of septal plugs is also associated with development and leads to separation of certain parts of the colony. Septal plugs associated with developmental processes or aging hyphae typically occur by the accumulation of sealing material. Here we report that in Neurospora crassa, a protein necessary for hyphal fusion and proper colony development called SO (SOFT) localizes to septal plugs. In response to injury, SO accumulates at the septal plug in a Woronin body-independent manner. However, the presence of the Woronin body affects the speed of accumulation of SO at the septal pore. We determined that SO contributes to, but is not essential for, septal plugging. SO accumulation was also observed at septal plugs formed during hyphal aging and during programmed cell death mediated by genetic differences at heterokaryon incompatibility (het) loci.

scholarly journals Woronin Body Function in Magnaporthe grisea Is Essential for Efficient Pathogenesis and for Survival during Nitrogen Starvation Stress

2004 ◽  
Vol 16 (6) ◽  
pp. 1564-1574 ◽  
Author(s):  
Shanthi Soundararajan ◽  
Gregory Jedd ◽  
Xiaolei Li ◽  
Marilou Ramos-Pamploña ◽  
Nam H. Chua ◽  
...  
Keyword(s):  
Magnaporthe Grisea ◽  
Nitrogen Starvation ◽  
Starvation Stress ◽  
Body Function ◽  
Woronin Body

Ultrastructure and Morphology of the Neurospora Crassa Cell Wall Mutants, Slime And Slime X, Using Tem and Sem

1976 ◽  
Vol 34 ◽  
pp. 54-55
Author(s):  
Karen S. Howard ◽  
H. D. Braymer ◽  
M. D. Socolofsky ◽  
S. A. Milligan
Keyword(s):  
Cell Wall ◽  
Neurospora Crassa ◽  
Wild Type ◽  
Morphological Comparison ◽  
Small Areas ◽  
Solid Media ◽  
Thin Sectioning ◽  
X Cells ◽  
Mutant Cells ◽  
Isolated Cell

The recently isolated cell wall mutant slime X of Neurospora crassa was prepared for ultrastructural and morphological comparison with the cell wall mutant slime. The purpose of this article is to discuss the methods of preparation for TEM and SEM observations, as well as to make a preliminary comparison of the two mutants.TEM: Cells of the slime mutant were prepared for thin sectioning by the method of Bigger, et al. Slime X cells were prepared in the same manner with the following two exceptions: the cells were embedded in 3% agar prior to fixation and the buffered solutions contained 5% sucrose throughout the procedure.SEM: Two methods were used to prepare mutant and wild type Neurospora for the SEM. First, single colonies of mutant cells and small areas of wild type hyphae were cut from solid media and fixed with OSO4 vapors similar to the procedure used by Harris, et al. with one alteration. The cell-containing agar blocks were dehydrated by immersion in 2,2-dimethoxypropane (DMP).

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