High-precision gene editing in monkeys is feasible

In 2015, the journal Science chose CRISPR-Cas9 technology as the most important technological advance of science in the last years. This magazine announced the beginning of a new era of biotechnology in which it would be possible to edit, correct and modify the genetic information of any cell in a feasible, fast and cheap way; and most importantly, with high precision. Its implementation in research laboratories in basic and applied sciences could help to develop therapeutic strategies for the health area with the main objective of healing diseases with a known genetic origin, and that until now have been impossible to cure

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Nanoparticle-based non-viral CRISPR delivery for enhanced immunotherapy

Chemical Communications ◽

10.1039/d1cc05999h ◽

2022 ◽

Author(s):

Hyunsu Shin ◽

Jaeyun Kim

Keyword(s):

High Precision ◽

Gene Editing ◽

Therapeutic Applications ◽

Crispr System

The CRISPR Cas9 system has received considerable attention due to its simplicity, efficiency, and high precision for gene editing. The development of various therapeutic applications of the CRISPR system is...

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Automatic measurement of SAED patterns from asbestos minerals

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100106296 ◽

1988 ◽

Vol 46 ◽

pp. 846-847

Author(s):

J. C. Russ ◽

T. Taguchi ◽

P. M. Peters ◽

E. Chatfield ◽

J. C. Russ ◽

...

Keyword(s):

Diffraction Pattern ◽

High Precision ◽

Diffraction Data ◽

Automatic Measurement ◽

Automatic Method ◽

Important Method ◽

X Ray ◽

Integrated Intensity ◽

Asbestiform Minerals ◽

True Center

Conventional SAD patterns as obtained in the TEM present difficulties for identification of materials such as asbestiform minerals, although diffraction data is considered to be an important method for making this purpose. The preferred orientation of the fibers and the spotty patterns that are obtained do not readily lend themselves to measurement of the integrated intensity values for each d-spacing, and even the d-spacings may be hard to determine precisely because the true center location for the broken rings requires estimation. We have implemented an automatic method for diffraction pattern measurement to overcome these problems. It automatically locates the center of patterns with high precision, measures the radius of each ring of spots in the pattern, and integrates the density of spots in that ring. The resulting spectrum of intensity vs. radius is then used just as a conventional X-ray diffractometer scan would be, to locate peaks and produce a list of d,I values suitable for search/match comparison to known or expected phases.

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On effects of non-systematic reflections on weak-beam images of partial dislocations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100087756 ◽

1991 ◽

Vol 49 ◽

pp. 688-689

Author(s):

K. Z. Botros ◽

S. S. Sheinin

Keyword(s):

High Precision ◽

Stacking Fault ◽

Important Application ◽

Stacking Fault Energy ◽

Sharp Peak ◽

Weak Beam ◽

Partial Dislocations ◽

Deviation Parameter ◽

Beam Diffraction

The main features of weak beam images of dislocations were first described by Cockayne et al. using calculations of intensity profiles based on the kinematical and two beam dynamical theories. The feature of weak beam images which is of particular interest in this investigation is that intensity profiles exhibit a sharp peak located at a position very close to the position of the dislocation in the crystal. This property of weak beam images of dislocations has an important application in the determination of stacking fault energy of crystals. This can easily be done since the separation of the partial dislocations bounding a stacking fault ribbon can be measured with high precision, assuming of course that the weak beam relationship between the positions of the image and the dislocation is valid. In order to carry out measurements such as these in practice the specimen must be tilted to "good" weak beam diffraction conditions, which implies utilizing high values of the deviation parameter Sg.

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Digital differential hysteresis iimage processing displays what the microscope acquires but the eye can't see

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100139585 ◽

1995 ◽

Vol 53 ◽

pp. 642-643

Author(s):

Klaus-Ruediger Peters

Keyword(s):

Image Processing ◽

Visual System ◽

High Precision ◽

Image Data ◽

Processing Technology ◽

Output Data ◽

Contrast Resolution ◽

Pixel Intensity ◽

Data Reading ◽

Hysteresis Properties

Differential hysteresis processing is a new image processing technology that provides a tool for the display of image data information at any level of differential contrast resolution. This includes the maximum contrast resolution of the acquisition system which may be 1,000-times higher than that of the visual system (16 bit versus 6 bit). All microscopes acquire high precision contrasts at a level of <0.01-25% of the acquisition range in 16-bit - 8-bit data, but these contrasts are mostly invisible or only partially visible even in conventionally enhanced images. The processing principle of the differential hysteresis tool is based on hysteresis properties of intensity variations within an image.Differential hysteresis image processing moves a cursor of selected intensity range (hysteresis range) along lines through the image data reading each successive pixel intensity. The midpoint of the cursor provides the output data. If the intensity value of the following pixel falls outside of the actual cursor endpoint values, then the cursor follows the data either with its top or with its bottom, but if the pixels' intensity value falls within the cursor range, then the cursor maintains its intensity value.

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