scholarly journals Telomere length measurement in single cells

2013 ◽  
Vol 14 (7) ◽  
pp. 444-444
Author(s):  
Hannah Stower
Keyword(s):  
Telomere Length ◽  
Single Cells ◽  
Length Measurement

scholarly journals Transcriptomic analyses and leukocyte telomere length measurement in subjects exposed to severe recent stressful life events

2017 ◽  
Vol 7 (2) ◽  
pp. e1042-e1042 ◽  
Author(s):  
N Lopizzo ◽  
S Tosato ◽  
V Begni ◽  
S Tomassi ◽  
N Cattane ◽  
...  
Keyword(s):  
Telomere Length ◽  
Life Events ◽  
Stressful Life Events ◽  
Length Measurement ◽  
Stressful Life ◽  
Leukocyte Telomere Length

Telomere length measurement in tumor and non‐tumor cells as a valuable prognostic for tumor progression

2019 ◽  
Vol 238 ◽  
pp. 50-61 ◽  
Author(s):  
Fatma Mehrez ◽  
Karim Bougatef ◽  
Elisa Delle Monache ◽  
Ivan Arisi ◽  
Luca Proietti-De-Santis ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Telomere Length ◽  
Tumor Cells ◽  
Length Measurement

Comparison of different methods for telomere length measurement in whole blood and blood cell subsets: Recommendations for telomere length measurement in hematological diseases

2017 ◽  
Vol 56 (9) ◽  
pp. 700-708 ◽  
Author(s):  
Yvonne Lisa Behrens ◽  
Kathrin Thomay ◽  
Maike Hagedorn ◽  
Juliane Ebersold ◽  
Lea Henrich ◽  
...  
Keyword(s):  
Blood Cell ◽  
Telomere Length ◽  
Whole Blood ◽  
Length Measurement ◽  
Hematological Diseases

scholarly journals Blood and Dried Blood Spot Telomere Length Measurement by qPCR: Assay Considerations

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e57787 ◽  
Author(s):  
DeAnna L. Zanet ◽  
Sara Saberi ◽  
Laura Oliveira ◽  
Beheroze Sattha ◽  
Izabella Gadawski ◽  
...  
Keyword(s):  
Telomere Length ◽  
Length Measurement ◽  
Qpcr Assay ◽  
Dried Blood Spot ◽  
Blood Spot

scholarly journals Reliability and Short-Term Intra-Individual Variability of Telomere Length Measurement Using Monochrome Multiplexing Quantitative PCR

PLoS ONE ◽  
2011 ◽  
Vol 6 (9) ◽  
pp. e25774 ◽  
Author(s):  
Sangmi Kim ◽  
Dale P. Sandler ◽  
Gleta Carswell ◽  
Clarice R. Weinberg ◽  
Jack A. Taylor
Keyword(s):  
Telomere Length ◽  
Quantitative Pcr ◽  
Individual Variability ◽  
Length Measurement ◽  
Short Term

scholarly journals ΩqPCR measures telomere length from single-cells in base pair units

2021 ◽  
Author(s):  
Fusheng Xiong ◽  
Wayne D Frasch
Keyword(s):  
Telomere Length ◽  
Single Cells ◽  
Measuring Method ◽  
Single Copy ◽  
Primer Binding Site ◽  
Sequence Information ◽  
Accuracy And Precision ◽  
Reverse Primer ◽  
Dna Strand ◽  
Forward Primer

Abstract ΩqPCR determines absolute telomere length in kb units from single cells. Accuracy and precision of ΩqPCR were assessed using 800 bp and 1600 bp synthetic telomeres inserted into plasmids, which were measured to be 819 ± 19.6 and 1590 ± 42.3 bp, respectively. This is the first telomere length measuring method verified in this way. The approach uses Ω-probes, a DNA strand containing sequence information that enables: (i) hybridization with the telomere via the 3′ and 5′ ends that become opposed; (ii) ligation of the hybridized probes to circularize the Ω-probes and (iii) circularized-dependent qPCR due to sequence information for a forward primer, and for a reverse primer binding site, and qPCR hydrolysis probe binding. Read through of the polymerase during qPCR occurs only in circularized Ω-probes, which quantifies their number that is directly proportional to telomere length. When used in concert with information about the cell cycle stage from a single-copy gene, and ploidy, the MTL of single cells measured by ΩqPCR was consistent with that obtained from large sample sizes by TRF.

scholarly journals Telomere Length Measurement by Molecular Combing

2020 ◽  
Vol 8 ◽  
Author(s):  
Vivian F. S. Kahl ◽  
Joshua A. M. Allen ◽  
Christopher B. Nelson ◽  
Alexander P. Sobinoff ◽  
Michael Lee ◽  
...  
Keyword(s):  
Telomere Length ◽  
Length Measurement ◽  
Molecular Combing
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