scholarly journals Genome-wide analysis of replication timing by next-generation sequencing with E/L Repli-seq

2018 ◽  
Vol 13 (5) ◽  
pp. 819-839 ◽  
Author(s):  
Claire Marchal ◽  
Takayo Sasaki ◽  
Daniel Vera ◽  
Korey Wilson ◽  
Jiao Sima ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Replication Timing ◽  
Next Generation ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Generation Sequencing

scholarly journals Repli-seq: genome-wide analysis of replication timing by next-generation sequencing

2017 ◽  
Author(s):  
Claire Marchal ◽  
Takayo Sasaki ◽  
Daniel Vera ◽  
Korey Wilson ◽  
Jiao Sima ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Replication Timing ◽  
Nucleotide Polymorphisms ◽  
Robust Methods ◽  
Next Generation ◽  
Single Nucleotide ◽  
Cellular Processes ◽  
Genome Wide ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

ABSTRACTCycling cells duplicate their DNA content during S phase, following a defined program called replication timing (RT). Early and late replicating regions differ in terms of mutation rates, transcriptional activity, chromatin marks and sub-nuclear position. Moreover, RT is regulated during development and is altered in disease. Exploring mechanisms linking RT to other cellular processes in normal and diseased cells will be facilitated by rapid and robust methods with which to measure RT genome wide. Here, we describe a rapid, robust and relatively inexpensive protocol to analyze genome-wide RT by next-generation sequencing (NGS). This protocol yields highly reproducible results across laboratories and platforms. We also provide computational pipelines for analysis, parsing phased genomes using single nucleotide polymorphisms (SNP) for analyzing RT allelic asynchrony, and for direct comparison to Repli-chip data obtained by analyzing nascent DNA by microarrays.

scholarly journals Genome-wide analysis of Chongqing native intersexual goats using next-generation sequencing

3 Biotech ◽  
2019 ◽  
Vol 9 (3) ◽  
Author(s):  
Guang-Xin E ◽  
Mei-Lan Jin ◽  
Yong-Ju Zhao ◽  
Xiang-Long Li ◽  
Lan-Hui Li ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Generation Sequencing
Sign in / Sign up

Export Citation Format

Share Document