scholarly journals A high-throughput in vivo screening method in the mouse for identifying regulators of metastatic colonization

2017 ◽  
Vol 12 (12) ◽  
pp. 2465-2477 ◽  
Author(s):  
Anneliese O Speak ◽  
Agnieszka Swiatkowska ◽  
Natasha A Karp ◽  
Mark J Arends ◽  
David J Adams ◽  
...  
Keyword(s):  
High Throughput ◽  
Screening Method ◽  
In Vivo Screening ◽  
Metastatic Colonization

Use of a high-throughput screen to identify Leptospira mutants unable to colonize the carrier host or cause disease in the acute model of infection

2013 ◽  
Vol 62 (10) ◽  
pp. 1601-1608 ◽  
Author(s):  
Renee A. Marcsisin ◽  
Thanatchaporn Bartpho ◽  
Dieter M. Bulach ◽  
Amporn Srikram ◽  
Rasana W. Sermswan ◽  
...  
Keyword(s):  
Mouse Model ◽  
Virulence Factors ◽  
High Throughput ◽  
High Throughput Screening ◽  
Acute Infection ◽  
Screening Method ◽  
Hamster Model ◽  
Genes Encoding ◽  
Transposon Mutants

The molecular basis for leptospirosis infection and colonization remains poorly understood, with no efficient methods available for screening libraries of mutants for attenuation. We analysed the attenuation of leptospiral transposon mutants in vivo using a high-throughput method by infecting animals with pooled sets of transposon mutants. A total of 95 mutants was analysed by this method in the hamster model of acute infection, and one mutant was identified as attenuated (M1233, lb058 mutant). All virulence factors identified in Leptospira to date have been characterized in the acute model of infection, neglecting the carrier host. To address this, a BALB/c mouse colonization model was established. The lb058 mutant and two mutants defective in LPS synthesis were colonization deficient in the mouse model. By applying the high-throughput screening method, a further five colonization-deficient mutants were identified for the mouse model; these included two mutants in genes encoding proteins with a predicted role in iron uptake (LB191/HbpA and LB194). Two attenuated mutants had transposon insertions in either la0589 or la2786 (encoding proteins of unknown function). The final attenuated mutant had an unexpected deletion of genes la0969–la0975 at the point of transposon insertion. This is the first description of defined, colonization-deficient mutants in a carrier host for Leptospira. These mutants were either not attenuated or only weakly attenuated in the hamster model of acute leptospirosis, thus illustrating that different factors that may be required in the carrier and acute models of leptospiral infection. High-throughput screening can reduce the number of animals used in virulence studies and increase the capacity to screen mutants for attenuation, thereby enhancing the likelihood of detecting unique virulence factors. A comparison of virulence factors required in the carrier and acute models of infection will help to unravel colonization and dissemination mechanisms of leptospirosis.

scholarly journals 542. Novel Barcode-Based In Vivo Screening Method for Generating De Novo AAV Serotypes for CNS-Directed Gene Therapy

2016 ◽  
Vol 24 ◽  
pp. S216-S217
Author(s):  
Marcus Davidsson ◽  
Gang Wang ◽  
Patrick Aldrin-Kirk ◽  
Morgan Hartnor ◽  
Tomas Björklund
Keyword(s):  
Gene Therapy ◽  
De Novo ◽  
Screening Method ◽  
In Vivo Screening

RRR-Tocopherols and their Acetates as a Possible Scavenger of Free Radicals Produced in the Skin upon UVA-Exposure – An in vivo Screening Method

Lipid / Fett ◽  
1992 ◽  
Vol 94 (1) ◽  
pp. 24-27 ◽  
Author(s):  
G. M. J. Beijersbergen van Henegouwen ◽  
H. De Vries ◽  
L. T. van Den Broeke ◽  
H. E. Junginger
Keyword(s):  
Free Radicals ◽  
Screening Method ◽  
In Vivo Screening

High throughput in vitro and in vivo screening of inland waters of Southern California

2017 ◽  
Vol 19 (9) ◽  
pp. 1142-1149 ◽  
Author(s):  
A. C. Mehinto ◽  
D. R. VanDervort ◽  
W. Lao ◽  
G. He ◽  
M. S. Denison ◽  
...  
Keyword(s):  
High Throughput ◽  
Southern California ◽  
Inland Waters ◽  
In Vivo Screening ◽  
Freshwater Streams ◽  
Screening Assays

High throughput in vitro and in vivo screening assays were combined to evaluate contaminant impacts on the health of freshwater streams.

scholarly journals An High-Throughput In Vivo Screening System to Select H3K4-Specific Histone Demethylase Inhibitors

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86002 ◽  
Author(s):  
Cecilia Mannironi ◽  
Marco Proietto ◽  
Francesca Bufalieri ◽  
Enrico Cundari ◽  
Angela Alagia ◽  
...  
Keyword(s):  
High Throughput ◽  
Histone Demethylase ◽  
Screening System ◽  
In Vivo Screening

scholarly journals High-throughput in vivo screening of targeted molecular imaging agents

2009 ◽  
Vol 106 (42) ◽  
pp. 17904-17909 ◽  
Author(s):  
M. K. J. Gagnon ◽  
S. H. Hausner ◽  
J. Marik ◽  
C. K. Abbey ◽  
J. F. Marshall ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
High Throughput ◽  
Imaging Agents ◽  
In Vivo Screening ◽  
Molecular Imaging Agents ◽  
Targeted Molecular Imaging

scholarly journals High-Throughput Screening and Identification of Human Adenovirus Type 5 Inhibitors

2021 ◽  
Vol 11 ◽  
Author(s):  
Xiaojing Wen ◽  
Li Zhang ◽  
Shan Zhao ◽  
Qiang Liu ◽  
Wenyi Guan ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Method ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Tissue Sections ◽  
Screening And Identification ◽  
New Treatment

Human adenovirus infections can develop into diffuse multi-organ diseases in young children and immunocompromised patients, and severe cases can lead to death. However, there are no approved antiviral drugs available to treat adenovirus diseases. In this study, a chemiluminescence-based, high-throughput screening (HTS) assay was developed and applied to screen human adenovirus 5(HAdV5)inhibitors from 1,813 approved drug library and 556 traditional Chinese medicine-sourced small-molecule compounds. We identified three compounds with in vitro anti-HAdV5 activities in the low-micromolar range (EC50 values 0.3-4.5 μM, selectivity index values 20-300) that also showed inhibitory effects on HAdV3. Cardamomin (CDM) had good anti-HAdV5 activity in vitro. Furthermore, three dilutions of CDM (150, 75, and 37.5 mg/kg/d) administered to BALB/c mouse models inhibited HAdV5-fluc infection at 1 day post-infection by 80% (p < 0.05), 76% (p < 0.05), and 58% (p < 0.05), respectively. HE-staining of pathological tissue sections of mice infected with a wildtype adenoviral strain showed that CDM had a protective effect on tissues, especially in the liver, and greatly inhibited virus-induced necrosis of liver tissue. Thus, CDM inhibits adenovirus replication in vivo and in vitro. This study established a high-throughput screening method for anti-HAdV5 drugs and demonstrated CDM to be a candidate for HAdV5 therapy, potentially providing a new treatment for patients infected with adenoviruses.

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