Genetically encoded releasable photo-cross-linking strategies for studying protein–protein interactions in living cells

2017 ◽  
Vol 12 (10) ◽  
pp. 2147-2168 ◽  
Author(s):  
Yi Yang ◽  
Haiping Song ◽  
Dan He ◽  
Shuai Zhang ◽  
Shizhong Dai ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Living Cells ◽  
Cross Linking ◽  
Protein Protein Interactions

scholarly journals Identification of Protein-Protein Interactions and Topologies in Living Cells with Chemical Cross-linking and Mass Spectrometry

2008 ◽  
Vol 8 (3) ◽  
pp. 409-420 ◽  
Author(s):  
Haizhen Zhang ◽  
Xiaoting Tang ◽  
Gerhard R. Munske ◽  
Nikola Tolic ◽  
Gordon A. Anderson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Interactions ◽  
Living Cells ◽  
Cross Linking ◽  
Protein Protein Interactions ◽  
Chemical Cross Linking

Genetically encoded selective cross-linkers and emerging applications

2020 ◽  
Vol 48 (4) ◽  
pp. 1807-1817
Author(s):  
Haiyan Ren
Keyword(s):  
Protein Interactions ◽  
Unnatural Amino Acids ◽  
Protein Complexes ◽  
Living Cells ◽  
Cross Linking ◽  
Protein Protein Interactions ◽  
New Class ◽  
Selective Reaction ◽  
Biological Studies ◽  
Lower Background

There has been a large amount of interest in the development of genetically encoded cross-linkers that target functional groups naturally present in cells. Recently, a new class of unnatural amino acids that specifically react with target residues were developed and genetically incorporated. The selective reaction shows higher cross-linking efficiency, lower background and predictable cross-linking sites. It has been applied to enhance protein/peptide stability, pinpoint protein–protein interactions, stabilize protein complexes, engineer covalent protein inhibitors, identify phosphatases in living cells, etc. These new covalent linkages provide excellent new tools for protein engineering and biological studies. Their applications in biotherapy will provide considerable opportunities for innovating and improving biomolecular medicines.

Inducing and Sensing Protein–Protein Interactions in Living Cells by Selective Cross-linking

2007 ◽  
Vol 119 (23) ◽  
pp. 4359-4362 ◽  
Author(s):  
Guillaume Lemercier ◽  
Susanne Gendreizig ◽  
Maik Kindermann ◽  
Kai Johnsson
Keyword(s):  
Protein Interactions ◽  
Living Cells ◽  
Cross Linking ◽  
Protein Protein Interactions

Inducing and Sensing Protein–Protein Interactions in Living Cells by Selective Cross-linking

2007 ◽  
Vol 46 (23) ◽  
pp. 4281-4284 ◽  
Author(s):  
Guillaume Lemercier ◽  
Susanne Gendreizig ◽  
Maik Kindermann ◽  
Kai Johnsson
Keyword(s):  
Protein Interactions ◽  
Living Cells ◽  
Cross Linking ◽  
Protein Protein Interactions

scholarly journals A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (2) ◽  
pp. e9344 ◽  
Author(s):  
Carina Banning ◽  
Jörg Votteler ◽  
Dirk Hoffmann ◽  
Herwig Koppensteiner ◽  
Martin Warmer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Protein Interactions ◽  
Living Cells ◽  
Protein Protein Interactions
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