Quantifying transcription factor–DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy

2017 ◽  
Vol 12 (7) ◽  
pp. 1458-1471 ◽  
Author(s):  
Ziqing Winston Zhao ◽  
Melanie D White ◽  
Yanina D Alvarez ◽  
Jennifer Zenker ◽  
Stephanie Bissiere ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Fluorescence Correlation Spectroscopy ◽  
Single Cells ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

scholarly journals In vivo fluorescence correlation spectroscopy analyses of FMBP ‐1, a silkworm transcription factor

FEBS Open Bio ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 106-125 ◽  
Author(s):  
Motosuke Tsutsumi ◽  
Hideki Muto ◽  
Shohei Myoba ◽  
Mai Kimoto ◽  
Akira Kitamura ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
In Vivo Fluorescence

scholarly journals Probing the kinetic landscape of Hox transcription factor–DNA binding in live cells by massively parallel Fluorescence Correlation Spectroscopy

2015 ◽  
Vol 138 ◽  
pp. 218-225 ◽  
Author(s):  
Dimitrios K. Papadopoulos ◽  
Aleksandar J. Krmpot ◽  
Stanko N. Nikolić ◽  
Robert Krautz ◽  
Lars Terenius ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Massively Parallel ◽  
Live Cells ◽  
Fluorescence Correlation

Active protein transport through plastid tubules: velocity quantified by fluorescence correlation spectroscopy

2000 ◽  
Vol 113 (22) ◽  
pp. 3921-3930 ◽  
Author(s):  
R.H. Kohler ◽  
P. Schwille ◽  
W.W. Webb ◽  
M.R. Hanson
Keyword(s):  
Active Transport ◽  
Fluorescence Correlation Spectroscopy ◽  
Protein Transport ◽  
Fluorescent Protein ◽  
Correlation Spectroscopy ◽  
Long Distance ◽  
Fluorescence Correlation ◽  
Photon Excitation ◽  
Green Fluorescent

Dynamic tubular projections emanate from plastids in certain cells of vascular plants and are especially prevalent in non-photosynthetic cells. Tubules sometimes connect two or more different plastids and can extend over long distances within a cell, observations that suggest that the tubules may function in distribution of molecules within, to and from plastids. In a new application of two-photon excitation (2PE) fluorescence correlation spectroscopy (FCS), we separated diffusion of fluorescent molecules from active transport in vivo. We quantified the velocities of diffusion versus active transport of green fluorescent protein (GFP) within plastid tubules and in the cytosol in vivo. GFP moves by 3-dimensional (3-D) diffusion both in the cytosol and plastid tubules, but diffusion in tubules is about 50 times and 100 times slower than in the cytosol and an aqueous solution, respectively. Unexpectedly larger GFP units within plastid tubules exhibited active transport with a velocity of about 0.12 microm/second. Active transport might play an important role in the long-distance distribution of large numbers of molecules within the highly viscous stroma of plastid tubules.

scholarly journals Monitoring the interaction between DNA and a transcription factor (MEF2A) using fluorescence correlation spectroscopy

2005 ◽  
Vol 328 (12) ◽  
pp. 1033-1040 ◽  
Author(s):  
Guillaume Octobre ◽  
Claudie Lemercier ◽  
Saadi Khochbin ◽  
Michel Robert-Nicoud ◽  
Catherine Souchier
Keyword(s):  
Transcription Factor ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation
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