Capturing suboptical dynamic structures in lipid bilayer patches formed from free-standing giant unilamellar vesicles

2017 ◽  
Vol 12 (8) ◽  
pp. 1563-1575 ◽  
Author(s):  
Tripta Bhatia ◽  
Flemming Cornelius ◽  
John H Ipsen
Keyword(s):  
Lipid Bilayer ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Free Standing ◽  
Dynamic Structures

Fluorescent probe partitioning in giant unilamellar vesicles of ‘lipid raft’ mixtures

2010 ◽  
Vol 430 (3) ◽  
pp. 415-423 ◽  
Author(s):  
Janos Juhasz ◽  
James H. Davis ◽  
Frances J. Sharom
Keyword(s):  
Fluorescent Probe ◽  
Lipid Bilayer ◽  
Fluorescent Probes ◽  
Phase Behaviour ◽  
Model Systems ◽  
Fluorescence Probes ◽  
Lipid Phase ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Lipid Mixture

Direct visualization of raft-like lo (liquid-ordered) domains in model systems and cells using microscopic techniques requires fluorescence probes with known partitioning preference for one of the phases present. However, fluorescent probes may display dissimilar partitioning preferences in different lipid sys-tems and can also affect the phase behaviour of the host lipid bilayer. Therefore a detailed understanding of the behaviour of fluorescent probes in defined lipid bilayer systems with known phase behaviour is essential before they can be used for identifying domain phase states. Using giant unilamellar vesicles composed of the ternary lipid mixture DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine)/DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine)/cholesterol, for which the phase behaviour is known, we examined nine commonly used fluorescent probes using confocal fluorescence microscopy. The partitioning preference of each probe was assigned either on the basis of quantification of the domain area fractions or by using a well-characterized ld (liquid-disordered)-phase marker. Fluorescent probes were examined both individually and using dual or triple labelling approaches. Most of the probes partitioned individually into the ld phase, whereas only NAP (naphtho[2,3-a]pyrene) and NBD-DPPE [1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl] preferred the lo phase. We found that Rh-DPPE (Lissamine™ rhodamine B–1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine) increased the miscibility transition temperature, Tmix. Interestingly, the partitioning of DiIC18 (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate) was influenced by Bodipy®-PC [2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexa-decanoyl-sn-glycero-3-phosphocholine]. The specific use of each of the fluorescent probes is determined by its photostability, partitioning preference, ability to detect lipid phase separations and induced change in Tmix. We demonstrate the importance of testing a specific fluorescent probe in a given model membrane system, rather than assuming that it labels a particular lipid phase.

Incorporation of quantum dots into the lipid bilayer of giant unilamellar vesicles and its stability

2012 ◽  
Vol 97 ◽  
pp. 37-42 ◽  
Author(s):  
Haeng Sub Wi ◽  
Seong Jin Kim ◽  
Kyuyong Lee ◽  
Sang Min Kim ◽  
Ho Soon Yang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Lipid Bilayer ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

scholarly journals Imaging the lipid bilayer of giant unilamellar vesicles using red-to-blue light upconversion

2015 ◽  
Vol 51 (44) ◽  
pp. 9137-9140 ◽  
Author(s):  
Sven H. C. Askes ◽  
Néstor López Mora ◽  
Rolf Harkes ◽  
Roman I. Koning ◽  
Bram Koster ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Blue Light ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Triplet Triplet Annihilation

Red-to-blue triplet–triplet annihilation upconversion was obtained in giant unilamellar vesicles.

Synergy between plant phenols and carotenoids in stabilizing lipid-bilayer membranes of giant unilamellar vesicles against oxidative destruction

Soft Matter ◽  
2020 ◽  
Vol 16 (7) ◽  
pp. 1792-1800 ◽  
Author(s):  
Yi-Ming Zhou ◽  
Xiao-Chen Liu ◽  
Yu-Qian Li ◽  
Peng Wang ◽  
Rui-Min Han ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Antioxidant Effect ◽  
Marcus Theory ◽  
Water Interface ◽  
Oxidative Destruction ◽  
Lipid Bilayer Membranes ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Bilayer Membranes ◽  
Plant Phenols

The synergistic antioxidant effect of plant phenols and carotenoids at the lipid–water interface was consistent with the Marcus theory.

scholarly journals Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi
Keyword(s):  
Phospholipid Fatty Acid ◽  
Enzymatic Reaction ◽  
Stimuli Responsive ◽  
Membrane Composition ◽  
Giant Unilamellar Vesicles ◽  
Physical Processes ◽  
Unilamellar Vesicles ◽  
Division Process ◽  
The Origin Of Life ◽  
Area Difference

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.

Domain Sorting in Giant Unilamellar Vesicles Adsorbed on Glass

Langmuir ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1082-1088
Author(s):  
Chiho Kataoka-Hamai ◽  
Kohsaku Kawakami
Keyword(s):  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles

scholarly journals Production of giant unilamellar vesicles and encapsulation of lyotropic nematic liquid crystals

Soft Matter ◽  
2021 ◽  
Author(s):  
Peng Bao ◽  
Daniel A. Paterson ◽  
Sally A. Peyman ◽  
J. Cliff Jones ◽  
Jonathan A. T. Sandoe ◽  
...  
Keyword(s):  
Liquid Crystals ◽  
Nematic Liquid Crystals ◽  
Double Emulsion ◽  
Lyotropic Liquid Crystals ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Emulsion Droplets ◽  
Double Emulsion Droplets

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).

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