scholarly journals Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates

2014 ◽  
Vol 10 (1) ◽  
pp. 134-148 ◽  
Author(s):  
Maya Tanase ◽  
Valerio Zolla ◽  
Cristina C Clement ◽  
Francesco Borghi ◽  
Aleksandra M Urbanska ◽  
...  
Keyword(s):  
Protein Aggregates ◽  
Total Cell ◽  
Hydrodynamic Size ◽  
Cell Lysates ◽  
Size Based Separation

Rapid and reliable preparation of total cell lysates from fission yeast

2006 ◽  
Author(s):  
Atsuko Shirai ◽  
Akihisa Matsuyama ◽  
Yoko Yashiroda ◽  
Ritsuko Arai ◽  
Minoru Yoshida
Keyword(s):  
Fission Yeast ◽  
Total Cell ◽  
Cell Lysates

Automatic Counting of Intra-Cellular Ribonucleo-Protein Aggregates in Saccharomyces cerevisiae Using a Textural Approach

2019 ◽  
Vol 25 (1) ◽  
pp. 164-179
Author(s):  
Ambroise Marin ◽  
Emmanuel Denimal ◽  
Lucie Bertheau ◽  
Stéphane Guyot ◽  
Ludovic Journaux ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fluorescent Protein ◽  
Protein Aggregates ◽  
Point Of View ◽  
Zernike Moment ◽  
Two Photon ◽  
Haralick Features ◽  
Green Fluorescent ◽  
Two Photon Fluorescence

AbstractIn the context of microbiology, recent studies show the importance of ribonucleo-protein aggregates (RNPs) for the understanding of mechanisms involved in cell responses to specific environmental conditions. The assembly and disassembly of aggregates is a dynamic process, the characterization of the stage of their evolution can be performed by the evaluation of their number. The aim of this study is to propose a method to automatically determine the count of RNPs. We show that the determination of a precise count is an issue by itself and hence, we propose three textural approaches: a classical point of view using Haralick features, a frequency point of view with generalized Fourier descriptors, and a structural point of view with Zernike moment descriptors (ZMD). These parameters are then used as inputs for a supervised classification in order to determine the most relevant. An experiment using a specific Saccharomyces cerevisiae strain presenting a fusion between a protein found in RNPs (PAB1) and the green fluorescent protein was performed to benchmark this approach. The fluorescence was observed with two-photon fluorescence microscopy. Results show that the textural approach, by mixing ZMD with Haralick features, allows for the characterization of the number of RNPs.

Characterization of Microorganisms and Biomarker Development from Global ESI-MS/MS Analyses of Cell Lysates

2000 ◽  
Vol 72 (11) ◽  
pp. 2475-2481 ◽  
Author(s):  
Fan Xiang ◽  
Gordon A. Anderson ◽  
Timothy D. Veenstra ◽  
Mary S. Lipton ◽  
Richard D. Smith
Keyword(s):  
Esi Ms ◽  
Cell Lysates

Use of electrophoretic techniques and MALDI–TOF MS for rapid and reliable characterization of bacteria: analysis of intact cells, cell lysates, and “washed pellets”

2013 ◽  
Vol 405 (10) ◽  
pp. 3165-3175 ◽  
Author(s):  
Jiří Šalplachta ◽  
Anna Kubesová ◽  
Dana Moravcová ◽  
Marie Vykydalová ◽  
Sándor Süle ◽  
...  
Keyword(s):  
Intact Cells ◽  
Maldi Tof Ms ◽  
Cell Lysates ◽  
Maldi Tof ◽  
Electrophoretic Techniques ◽  
Tof Ms

Effect of protein aggregates on characterization of FcRn binding of Fc-fusion therapeutics

2015 ◽  
Vol 67 (2) ◽  
pp. 616-624 ◽  
Author(s):  
Adriana Bajardi-Taccioli ◽  
Andrew Blum ◽  
Chongfeng Xu ◽  
Zoran Sosic ◽  
Svetlana Bergelson ◽  
...  
Keyword(s):  
Protein Aggregates

Characterization of the fragmentation of protein aggregates in suspension subjected to flow

2007 ◽  
Vol 62 (22) ◽  
pp. 6440-6450 ◽  
Author(s):  
N. Péron ◽  
S.P. Heffernan ◽  
E.P. Byrne ◽  
F. Rioual ◽  
J.J. Fitzpatrick
Keyword(s):  
Protein Aggregates

scholarly journals Evaluation of nanoparticle tracking for characterization of fibrillar protein aggregates

AIChE Journal ◽  
2014 ◽  
Vol 60 (4) ◽  
pp. 1236-1244 ◽  
Author(s):  
Dennis T. Yang ◽  
Xiaomeng Lu ◽  
Yamin Fan ◽  
Regina M. Murphy
Keyword(s):  
Protein Aggregates
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