Generation of living cell arrays for atomic force microscopy studies

2014 ◽  
Vol 10 (1) ◽  
pp. 199-204 ◽  
Author(s):  
Cécile Formosa ◽  
Flavien Pillet ◽  
Marion Schiavone ◽  
Raphaël E Duval ◽  
Laurence Ressier ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Arrays

Living cell study at the single-molecule and single-cell levels by atomic force microscopy

Nanomedicine ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. 1625-1637 ◽  
Author(s):  
Xiaoli Shi ◽  
Xuejie Zhang ◽  
Tie Xia ◽  
Xiaohong Fang
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Single Molecule ◽  
Living Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Study

Study on measurement interaction force on living cell by atomic force microscopy

2005 ◽  
Author(s):  
Yanxia Wang ◽  
Yanning Li ◽  
Xing Fu ◽  
Jun-Yong Cui ◽  
Xiaotang Hu
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cell ◽  
Interaction Force ◽  
Force Microscopy ◽  
Atomic Force

Mechanical sensing of the penetration of various nanoneedles into a living cell using atomic force microscopy

2005 ◽  
Vol 20 (8) ◽  
pp. 1652-1655 ◽  
Author(s):  
Ikuo Obataya ◽  
Chikashi Nakamura ◽  
SungWoong Han ◽  
Noriyuki Nakamura ◽  
Jun Miyake
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Mechanical Sensing

scholarly journals Extending applications of AFM to fluidic AFM in single living cell and extracellular vesicle studies

2021 ◽  
Author(s):  
Yuan Qiu ◽  
Chen-Chi Chien ◽  
Basilis Maroulis ◽  
Angelo Gaitas ◽  
Bin Gong
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Living Cell ◽  
Cell Monolayer ◽  
Extracellular Vesicle ◽  
Liquid Environment ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Living Cell ◽  
Single Living

Abstract In this article, a review of the application of atomic force microscopy (AFM) for the analyses of extracellular vesicles is presented. This information is then extended to include fluidic Atomic Force Microscopy (fluidic AFM) applications. Fluidic AFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. AFM applications involving single cell and extracellular vesicle studies, colloidal force spectroscopy, and single cell adhesion measurements are discussed. In this review, new results are offered, using fluidic AFM, to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces (LBFs) of endothelial or epithelial cells in a confluent cell monolayer in appropriate physiological environment, and (3) the ease of measurement of vertical binding force (VBFs) of intercellular adhesion between heterogeneous cells. Finally, key applications are discussed that include extracellular vesicle absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.

Application of Atomic Force Microscopy to the Study of Expressed Molecules in or on a Single Living Cell

2009 ◽  
pp. 555-581
Author(s):  
Hyonchol Kim ◽  
Hironori Uehara ◽  
Rehana Afrin ◽  
Hiroshi Sekiguchi ◽  
Hideo Arakawa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Living Cell ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Living Cell ◽  
Single Living

Adhesion strength of a living cell to various substrates measured using a cup-attached atomic force microscopy chip

2017 ◽  
Vol 57 (3S2) ◽  
pp. 03EB01 ◽  
Author(s):  
Hyonchol Kim ◽  
Kenta Ishibashi ◽  
Kosuke Matsuo ◽  
Atsushi Kira ◽  
Yui Onomura ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Adhesion Strength ◽  
Living Cell ◽  
Force Microscopy ◽  
Atomic Force

The force of transporting a single amino acid into the living cell measured using atomic force microscopy

2013 ◽  
Vol 49 (74) ◽  
pp. 8163 ◽  
Author(s):  
Xin Shang ◽  
Yuping Shan ◽  
Yangang Pan ◽  
Mingjun Cai ◽  
Junguang Jiang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Amino Acid ◽  
Living Cell ◽  
Single Amino Acid ◽  
Force Microscopy ◽  
Atomic Force
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