Semiautomated correlative 3D electron microscopy of in vivo–imaged axons and dendrites

2014 ◽  
Vol 9 (6) ◽  
pp. 1354-1366 ◽  
Author(s):  
Bohumil Maco ◽  
Marco Cantoni ◽  
Anthony Holtmaat ◽  
Anna Kreshuk ◽  
Fred A Hamprecht ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
3D Electron Microscopy ◽  
3D Electron

scholarly journals Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision

2011 ◽  
Vol 192 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Wanda Kukulski ◽  
Martin Schorb ◽  
Sonja Welsch ◽  
Andrea Picco ◽  
Marko Kaksonen ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Fluorescent Protein ◽  
High Sensitivity ◽  
Cellular Processes ◽  
Cellular Events ◽  
Time Period ◽  
Fluorescence And Electron Microscopy ◽  
3D Electron Microscopy ◽  
3D Electron

Correlative electron and fluorescence microscopy has the potential to elucidate the ultrastructural details of dynamic and rare cellular events, but has been limited by low precision and sensitivity. Here we present a method for direct mapping of signals originating from ∼20 fluorescent protein molecules to 3D electron tomograms with a precision of less than 100 nm. We demonstrate that this method can be used to identify individual HIV particles bound to mammalian cell surfaces. We also apply the method to image microtubule end structures bound to mal3p in fission yeast, and demonstrate that growing microtubule plus-ends are flared in vivo. We localize Rvs167 to endocytic sites in budding yeast, and show that scission takes place halfway through a 10-s time period during which amphiphysins are bound to the vesicle neck. This new technique opens the door for direct correlation of fluorescence and electron microscopy to visualize cellular processes at the ultrastructural scale.

Visualizing membrane traffic in vivo by combined video fluorescence and 3D electron microscopy

2000 ◽  
Vol 10 (8) ◽  
pp. 349-353 ◽  
Author(s):  
Alexander A Mironov ◽  
Roman S Polishchuk ◽  
Alberto Luini
Keyword(s):  
Electron Microscopy ◽  
Membrane Traffic ◽  
3D Electron Microscopy ◽  
3D Electron

scholarly journals Patterns of organelle ontogeny through a cell cycle revealed by whole-cell reconstructions using 3D electron microscopy

Development ◽  
2017 ◽  
Vol 144 (4) ◽  
pp. e1.2-e1.2
Author(s):  
Louise Hughes ◽  
Samantha Borrett ◽  
Katie Towers ◽  
Tobias Starborg ◽  
Sue Vaughan
Keyword(s):  
Electron Microscopy ◽  
Cell Cycle ◽  
Whole Cell ◽  
3D Electron Microscopy ◽  
A Cell ◽  
3D Electron

scholarly journals Neural Modeling of Eukaryotic Cell Ultrastructure Obtained from 3D Electron Microscopy

2020 ◽  
Vol 118 (3) ◽  
pp. 293a
Author(s):  
Matthew D. Guay ◽  
Ruida Cheng ◽  
Zeyad A. Emam ◽  
Richard D. Leapman
Keyword(s):  
Electron Microscopy ◽  
Eukaryotic Cell ◽  
Cell Ultrastructure ◽  
Neural Modeling ◽  
3D Electron Microscopy ◽  
3D Electron

scholarly journals Improved metrics for comparing structures of macromolecular assemblies determined by 3D electron-microscopy

2017 ◽  
Vol 199 (1) ◽  
pp. 12-26 ◽  
Author(s):  
Agnel Praveen Joseph ◽  
Ingvar Lagerstedt ◽  
Ardan Patwardhan ◽  
Maya Topf ◽  
Martyn Winn
Keyword(s):  
Electron Microscopy ◽  
Macromolecular Assemblies ◽  
3D Electron Microscopy ◽  
3D Electron
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