Time-lapse X-ray phase-contrast microtomography for in vivo imaging and analysis of morphogenesis

2014 ◽  
Vol 9 (2) ◽  
pp. 294-304 ◽  
Author(s):  
Julian Moosmann ◽  
Alexey Ershov ◽  
Venera Weinhardt ◽  
Tilo Baumbach ◽  
Maneeshi S Prasad ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Phase Contrast ◽  
Time Lapse ◽  
X Ray

In-Vivo Imaging of Cancer Implanted in Nude Mice by Two-Crystal Interferometer-Based Phase-Contrast X-Ray Computed Tomography

2004 ◽  
Vol 43 (No. 9A/B) ◽  
pp. L1144-L1146 ◽  
Author(s):  
Tohoru Takeda ◽  
Akio Yoneyama ◽  
Jin Wu ◽  
Thet Thet Lwin ◽  
Yoshinori Tsuchiya ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Nude Mice ◽  
In Vivo Imaging ◽  
Phase Contrast ◽  
X Ray ◽  
X Ray Computed

scholarly journals In Vivo Imaging of Lung Tumor Growth Using In-Line X-Ray Phase Contrast Technique

2016 ◽  
Vol 3 (1) ◽  
pp. 174-176
Author(s):  
Rongbiao Tang ◽  
◽  
Fuhua Yan ◽  
Guo-Yuan Yang ◽  
Ke-Min Chen
Keyword(s):  
Tumor Growth ◽  
In Vivo Imaging ◽  
Phase Contrast ◽  
Lung Tumor ◽  
X Ray ◽  
Contrast Technique

scholarly journals The versatile X-ray beamline of the Munich Compact Light Source: design, instrumentation and applications

2020 ◽  
Vol 27 (5) ◽  
pp. 1395-1414 ◽  
Author(s):  
Benedikt Günther ◽  
Regine Gradl ◽  
Christoph Jud ◽  
Elena Eggl ◽  
Juanjuan Huang ◽  
...  
Keyword(s):  
Light Source ◽  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
Inverse Compton Scattering ◽  
University Of Munich ◽  
Inverse Compton ◽  
Compact Light Source

Inverse Compton scattering provides means to generate low-divergence partially coherent quasi-monochromatic, i.e. synchrotron-like, X-ray radiation on a laboratory scale. This enables the transfer of synchrotron techniques into university or industrial environments. Here, the Munich Compact Light Source is presented, which is such a compact synchrotron radiation facility based on an inverse Compton X-ray source (ICS). The recent improvements of the ICS are reported first and then the various experimental techniques which are most suited to the ICS installed at the Technical University of Munich are reviewed. For the latter, a multipurpose X-ray application beamline with two end-stations was designed. The beamline's design and geometry are presented in detail including the different set-ups as well as the available detector options. Application examples of the classes of experiments that can be performed are summarized afterwards. Among them are dynamic in vivo respiratory imaging, propagation-based phase-contrast imaging, grating-based phase-contrast imaging, X-ray microtomography, K-edge subtraction imaging and X-ray spectroscopy. Finally, plans to upgrade the beamline in order to enhance its capabilities are discussed.

scholarly journals Towards automated in vivo tracheal mucociliary transport measurement: Detecting and tracking particle movement in synchrotron phase-contrast x-ray images

2020 ◽  
Vol 65 (14) ◽  
pp. 145012
Author(s):  
Mark Gardner ◽  
David Parsons ◽  
Kaye Morgan ◽  
Alexandra McCarron ◽  
Patricia Cmielewski ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Mucociliary Transport ◽  
Particle Movement ◽  
Transport Measurement ◽  
X Ray

scholarly journals Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rhiannon P. Murrie ◽  
Freda Werdiger ◽  
Martin Donnelley ◽  
Yu-wei Lin ◽  
Richard P. Carnibella ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Lung Function ◽  
Mouse Model ◽  
Real Time ◽  
In Vivo Imaging ◽  
X Ray ◽  
Regional Lung Function ◽  
Regional Lung

scholarly journals A proof-of principal study using phase-contrast imaging for the detection of large airway pathologies after lung transplantation

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Stephan Umkehrer ◽  
Carmela Morrone ◽  
Julien Dinkel ◽  
Laura Aigner ◽  
Maximilian F. Reiser ◽  
...  
Keyword(s):  
Lung Transplantation ◽  
Phase Contrast ◽  
Small Animal ◽  
Lung Parenchyma ◽  
Dark Field ◽  
Contrast Imaging ◽  
X Ray ◽  
Contrast Mechanism ◽  
Contrast Information

Abstract In this study we aim to evaluate the assessment of bronchial pathologies in a murine model of lung transplantation with grating-based X-ray interferometry in vivo. Imaging was performed using a dedicated grating-based small-animal X-ray dark-field and phase-contrast scanner. While the contrast modality of the dark-field signal already showed several promising applications for diagnosing various types of pulmonary diseases, the phase-shifting contrast mechanism of the phase contrast has not yet been evaluated in vivo. For this purpose, qualitative analysis of phase-contrast images was performed and revealed pathologies due to previous lung transplantation, such as unilateral bronchial stenosis or bronchial truncation. Dependent lung parenchyma showed a strong loss in dark-field and absorption signal intensity, possibly caused by several post transplantational pathologies such as atelectasis, pleural effusion, or pulmonary infiltrates. With this study, we are able to show that bronchial pathologies can be visualized in vivo using conventional X-ray imaging when phase-contrast information is analysed. Absorption and dark-field images can be used to quantify the severity of lack of ventilation in the affected lung.

scholarly journals Long-term in vivo time-lapse imaging of synapse development and plasticity in the cerebellum

2014 ◽  
Vol 111 (1) ◽  
pp. 208-216 ◽  
Author(s):  
Naoko Nishiyama ◽  
Jeremy Colonna ◽  
Elise Shen ◽  
Jennifer Carrillo ◽  
Hiroshi Nishiyama
Keyword(s):  
In Vivo Imaging ◽  
Time Window ◽  
Time Lapse ◽  
Mammalian Brain ◽  
Cerebellar Neurons ◽  
Brain Functions ◽  
Time Lapse Imaging ◽  
Synaptic Circuitry

Synapses are continuously formed and eliminated throughout life in the mammalian brain, and emerging evidence suggests that this structural plasticity underlies experience-dependent changes of brain functions such as learning and long-term memory formation. However, it is generally difficult to understand how the rewiring of synaptic circuitry observed in vivo eventually relates to changes in animal's behavior. This is because afferent/efferent connections and local synaptic circuitries are very complicated in most brain regions, hence it is largely unclear how sensorimotor information is conveyed, integrated, and processed through a brain region that is imaged. The cerebellar cortex provides a particularly useful model to challenge this problem because of its simple and well-defined synaptic circuitry. However, owing to the technical difficulty of chronic in vivo imaging in the cerebellum, it remains unclear how cerebellar neurons dynamically change their structures over a long period of time. Here, we showed that the commonly used method for neocortical in vivo imaging was not ideal for long-term imaging of cerebellar neurons, but simple optimization of the procedure significantly improved the success rate and the maximum time window of chronic imaging. The optimized method can be used in both neonatal and adult mice and allows time-lapse imaging of cerebellar neurons for more than 5 mo in ∼80% of animals. This method allows vital observation of dynamic cellular processes such as developmental refinement of synaptic circuitry as well as long-term changes of neuronal structures in adult cerebellum under longitudinal behavioral manipulations.

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