Three-dimensional cell culturing by magnetic levitation

2013 ◽  
Vol 8 (10) ◽  
pp. 1940-1949 ◽  
Author(s):  
William L Haisler ◽  
David M Timm ◽  
Jacob A Gage ◽  
Hubert Tseng ◽  
T C Killian ◽  
...  
Keyword(s):  
Magnetic Levitation ◽  
Three Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell

scholarly journals Correction: Scaffold-free three-dimensional cell culturing using magnetic levitation

2018 ◽  
Vol 6 (7) ◽  
pp. 1996-1996
Author(s):  
Esra Türker ◽  
Nida Demirçak ◽  
Ahu Arslan-Yildiz
Keyword(s):  
Magnetic Levitation ◽  
Three Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell

Correction for ‘Scaffold-free three-dimensional cell culturing using magnetic levitation’ by Esra Türker et al., Biomater. Sci., 2018, DOI: 10.1039/c8bm00122g.

scholarly journals Scaffold-free three-dimensional cell culturing using magnetic levitation

2018 ◽  
Vol 6 (7) ◽  
pp. 1745-1753 ◽  
Author(s):  
Esra Türker ◽  
Nida Demirçak ◽  
Ahu Arslan-Yildiz
Keyword(s):  
Cell Culture ◽  
Magnetic Levitation ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
3D Structures ◽  
Cell Culturing ◽  
Culture Formation ◽  
Dimensional Cell

Magnetic levitation platform ensures a scaffold-free 3D cell culture formation by utilizing Gadolinium(iii) chelates, which provide paramagnetic environment for levitation; therefore, the cells are assembled into complex 3D structures.

Three-dimensional cell culturing by magnetic levitation for evaluating efficacy/toxicity of photodynamic therapy

2014 ◽  
Author(s):  
Luis G. Sabino ◽  
Priscila F. C. Menezes ◽  
Vanderlei S. Bagnato ◽  
Glauco Souza ◽  
Thomas C. Killian ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Magnetic Levitation ◽  
Three Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

scholarly journals Growing, Tracking, and Directing Bone Marrow Derived Stem Cells From Two-Dimensional and Three-Dimensional Cell Culture Microenvironments

2019 ◽  
Author(s):  
Tiffany Miller
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Stem Cell ◽  
Three Dimensional ◽  
Inflammatory Biomarkers ◽  
Two Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell ◽  
Stimulation Of

<p>Bone marrow derived stem cells express biomarkers capable of facilitating adhesion to the cell culturing microenvironment, thereby, influencing their proliferation, migration, and differentiation. In particular, biological biomarkers of mesenchymal stem cells include, but are not limited to, CD14-, CD19-, CD34-, CD45-, CD29, CD44, CD73+, CD90+, CD105+, CD106, CD166, Stro-1, and HLADR. The relationship between the stem cell biology and the materials and methods forming a cell culturing microenvironment serves as a critical aspect in the successful adhesion and growth within two-dimensional cell culture microenvironments such as polystyrene, laminin, fibronectin, or poly-L-lysine and within three-dimensional cell culture microenvironments such as hydrogel, ceramic, collagen, polymer based nanofibers, agitation, forced floating, and hang drop systems. Further, electrical stimulation of the stem cells may be implemented during the cell culturing process to measure stem cell growth and to determine stem cell viability. In addition, electrical stimulation of implanted stem cells may facilitate tracking by measuring stem cell migration distance and travel area. Although many biochemical and inflammatory biomarkers are expressed based on severity in stroke including, but not limited to, Interluken-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glutamate (Glu), current methodologies of stem cell directing lack localization and biological effector specificity. Biological effector bound magnetic particle stem cells may serve as a potential treatment method in ischemic stroke. In particular, a stem cell biomarker may be configured to communicate with inflammatory biomarkers, thus, more efficiently delivering the stem cells to site specific areas having the most severely affected <i>in-vivo</i> biochemical microenvironments.</p>

scholarly journals Growing, Tracking, and Directing Bone Marrow Derived Stem Cells From Two-Dimensional and Three-Dimensional Cell Culture Microenvironments

2019 ◽  
Author(s):  
Tiffany Miller
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Stem Cell ◽  
Three Dimensional ◽  
Inflammatory Biomarkers ◽  
Two Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell ◽  
Stimulation Of

<p>Bone marrow derived stem cells express biomarkers capable of facilitating adhesion to the cell culturing microenvironment, thereby, influencing their proliferation, migration, and differentiation. In particular, biological biomarkers of mesenchymal stem cells include, but are not limited to, CD14-, CD19-, CD34-, CD45-, CD29, CD44, CD73+, CD90+, CD105+, CD106, CD166, Stro-1, and HLADR. The relationship between the stem cell biology and the materials and methods forming a cell culturing microenvironment serves as a critical aspect in the successful adhesion and growth within two-dimensional cell culture microenvironments such as polystyrene, laminin, fibronectin, or poly-L-lysine and within three-dimensional cell culture microenvironments such as hydrogel, ceramic, collagen, polymer based nanofibers, agitation, forced floating, and hang drop systems. Further, electrical stimulation of the stem cells may be implemented during the cell culturing process to measure stem cell growth and to determine stem cell viability. In addition, electrical stimulation of implanted stem cells may facilitate tracking by measuring stem cell migration distance and travel area. Although many biochemical and inflammatory biomarkers are expressed based on severity in stroke including, but not limited to, Interluken-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glutamate (Glu), current methodologies of stem cell directing lack localization and biological effector specificity. Biological effector bound magnetic particle stem cells may serve as a potential treatment method in ischemic stroke. In particular, a stem cell biomarker may be configured to communicate with inflammatory biomarkers, thus, more efficiently delivering the stem cells to site specific areas having the most severely affected <i>in-vivo</i> biochemical microenvironments.</p>

Evaluation of photodynamic therapy in three-dimensional cell culture by magnetic levitation

2015 ◽  
Vol 12 (3) ◽  
pp. 351
Author(s):  
Larissa Satiko Alcântara Sekimoto ◽  
Luis Gustavo Sabino ◽  
Layla Pires ◽  
Cristina Kurachi
Keyword(s):  
Cell Culture ◽  
Photodynamic Therapy ◽  
Magnetic Levitation ◽  
Three Dimensional ◽  
Dimensional Cell
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