Generation of transgenic mice with megabase-sized human yeast artificial chromosomes by yeast spheroplast–embryonic stem cell fusion

2013 ◽  
Vol 8 (8) ◽  
pp. 1567-1582 ◽  
Author(s):  
Liangping Li ◽  
Thomas Blankenstein
Keyword(s):  
Stem Cell ◽  
Transgenic Mice ◽  
Embryonic Stem Cell ◽  
Cell Fusion ◽  
Embryonic Stem ◽  
Artificial Chromosomes ◽  
Yeast Artificial Chromosomes

scholarly journals G9a and Jhdm2a Regulate Embryonic Stem Cell Fusion-Induced Reprogramming of Adult Neural Stem Cells

Stem Cells ◽  
2008 ◽  
Vol 26 (8) ◽  
pp. 2131-2141 ◽  
Author(s):  
Dengke K. Ma ◽  
Cheng-Hsuan J. Chiang ◽  
Karthikeyan Ponnusamy ◽  
Guo-li Ming ◽  
Hongjun Song
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Embryonic Stem Cell ◽  
Cell Fusion ◽  
Embryonic Stem ◽  
Adult Neural Stem Cells

scholarly journals Generation of Embryonic Stem Cell-derived Transgenic Mice by Using Tetraploid Complementation

2004 ◽  
Vol 17 (12) ◽  
pp. 1641-1646 ◽  
Author(s):  
S. M. Park ◽  
S. J. Song ◽  
S. J. Uhm ◽  
S. G. Cho ◽  
S. P. Park ◽  
...  
Keyword(s):  
Stem Cell ◽  
Transgenic Mice ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Tetraploid Complementation

Germ line transmission of yeast artificial chromosomes in transgenic mice

1994 ◽  
Vol 6 (5) ◽  
pp. 577 ◽  
Author(s):  
L Montoliu ◽  
A Schedl ◽  
G Kelsey ◽  
H Zentgraf ◽  
P Lichter ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Germ Line ◽  
Es Cells ◽  
Embryonic Stem ◽  
Artificial Chromosomes ◽  
Tyrosinase Gene ◽  
Temporal And Spatial ◽  
Genomic Regions ◽  
Germ Line Transmission ◽  
Yeast Artificial Chromosomes

Several groups have recently reported the successful generation of transgenic mice harbouring yeast artificial chromosomes (YACs). Different methodological approaches have been shown to produce similar results, namely, the faithful expression of the transgenes carried on YAC DNA. In this paper, we compare the reported techniques for obtaining transgenic mice carrying YACs using a 250-kb YAC bearing the mouse tyrosinase gene. These methods include: microinjection of gel-purified YAC DNA into pronuclei of fertilized mouse oocytes, yeast spheroblast fusion with embryonic stem (ES) cells and lipofection of YAC DNA into ES cells. Taken together, these reports show that the delivery of large genomic regions covering a gene of interest (such as those cloned in YAC vectors) is feasible, and will ensure appropriate temporal and spatial expression of the transgene at a level comparable to that of the endogenous counterpart.

Interspecies Cell Fusion between Mouse Embryonic Stem Cell and Porcine Pluripotent Cell

2021 ◽  
Author(s):  
Gerelchimeg Bou ◽  
Jia Guo ◽  
Yuan Fang ◽  
Xuechun Li ◽  
Renyue Wei ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Fusion ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Pluripotent Cell

Potential Use of Enucleated Embryonic Stem Cell Cytoplasts in Investigations of Stem Cell Fusion

2004 ◽  
Vol 13 (2) ◽  
pp. 165-167 ◽  
Author(s):  
C. Mantel ◽  
Y. Guo ◽  
C. Kauffman ◽  
D. English ◽  
H.E. Broxmeyer
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Fusion ◽  
Embryonic Stem ◽  
Potential Use

Transgenic mice for the preparation of puromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selection

2001 ◽  
Vol 12 (2) ◽  
pp. 169-171 ◽  
Author(s):  
Elizabeth R. Linnell ◽  
Charles P. Lerner ◽  
Kevin A. Johnson ◽  
Craig A. Leach ◽  
Thomas R. Ulrich ◽  
...  
Keyword(s):  
Stem Cell ◽  
Transgenic Mice ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cell Selection ◽  
Feeder Layers ◽  
Embryonic Fibroblast

scholarly journals Transgenic mice for the preparation of hygromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selections

1995 ◽  
Vol 23 (7) ◽  
pp. 1273-1275 ◽  
Author(s):  
Kevin A. Johnson ◽  
Charles P. Lerner ◽  
Lena C. Di Lacio ◽  
Peter W. Laird ◽  
Arlene H. Sharpe ◽  
...  
Keyword(s):  
Stem Cell ◽  
Transgenic Mice ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Feeder Layers ◽  
Embryonic Fibroblast
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