scholarly journals Design and implementation of in vivo imaging of neural injury responses in the adult Drosophila wing

2013 ◽  
Vol 8 (4) ◽  
pp. 810-819 ◽  
Author(s):  
Yanshan Fang ◽  
Lorena Soares ◽  
Nancy M Bonini
Keyword(s):  
In Vivo Imaging ◽  
Neural Injury ◽  
Design And Implementation ◽  
Drosophila Wing ◽  
Adult Drosophila

scholarly journals In vivo imaging of Drosophila wing heart development during pupal stages

2013 ◽  
Vol 57 (1) ◽  
pp. 13-24 ◽  
Author(s):  
Markus Tögel ◽  
Günther Pass ◽  
Achim Paululat
Keyword(s):  
In Vivo Imaging ◽  
Heart Development ◽  
Drosophila Wing

scholarly journals In-Vivo Imaging of the Drosophila Wing Imaginal Disc over Time: Novel Insights on Growth and Boundary Formation

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47594 ◽  
Author(s):  
Ulrike Nienhaus ◽  
Tinri Aegerter-Wilmsen ◽  
Christof M. Aegerter
Keyword(s):  
In Vivo Imaging ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Boundary Formation ◽  
Drosophila Wing ◽  
Over Time

scholarly journals In Vivo Imaging of the Adult Drosophila melanogaster Heart With Real-Time Optical Coherence Tomography

Circulation ◽  
2006 ◽  
Vol 114 (2) ◽  
Author(s):  
Michael A. Choma ◽  
Susan D. Izatt ◽  
Robert J. Wessells ◽  
Rolf Bodmer ◽  
Joseph A. Izatt
Keyword(s):  
Optical Coherence Tomography ◽  
Drosophila Melanogaster ◽  
Real Time ◽  
In Vivo Imaging ◽  
Optical Coherence ◽  
Adult Drosophila

scholarly journals Microfluidic Chips for In Vivo Imaging of Cellular Responses to Neural Injury in Drosophila Larvae

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e29869 ◽  
Author(s):  
Mostafa Ghannad-Rezaie ◽  
Xing Wang ◽  
Bibhudatta Mishra ◽  
Catherine Collins ◽  
Nikos Chronis
Keyword(s):  
In Vivo Imaging ◽  
Cellular Responses ◽  
Microfluidic Chips ◽  
Neural Injury ◽  
Drosophila Larvae

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

In vivo imaging reveals prostate pathology in the PTEN knockout murine model of prostate cancer

2016 ◽  
Author(s):  
Alysha Bhatti ◽  
Almeida Gilberto Serrano de ◽  
Serena Tommasini Ghelfi ◽  
Alwyn Dart ◽  
Anabel Varela-Carver ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Murine Model ◽  
In Vivo Imaging
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