scholarly journals Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis

2013 ◽  
Vol 8 (5) ◽  
pp. 924-934 ◽  
Author(s):  
Michael J Layden ◽  
Eric Röttinger ◽  
Francis S Wolenski ◽  
Thomas D Gilmore ◽  
Mark Q Martindale
Keyword(s):  
Genetic Analysis ◽  
Sea Anemone ◽  
Nematostella Vectensis ◽  
Reverse Genetic

scholarly journals Reverse Genetic Analysis of the Transcription Regulatory Sequence of the Coronavirus Transmissible Gastroenteritis Virus

2004 ◽  
Vol 78 (11) ◽  
pp. 6061-6066 ◽  
Author(s):  
Kristopher M. Curtis ◽  
Boyd Yount ◽  
Amy C. Sims ◽  
Ralph S. Baric
Keyword(s):  
Genetic Analysis ◽  
Full Length ◽  
Transmissible Gastroenteritis Virus ◽  
Regulatory Sequence ◽  
Reverse Genetic ◽  
Conserved Sequence ◽  
Gastroenteritis Virus ◽  
Flanking Sequences ◽  
Transmissible Gastroenteritis ◽  
Discontinuous Transcription

ABSTRACT Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.

Starlet Sea Anemone (Nematostella vectensis) 2021 Environmental Summary, Reptile & Aquatics, Stowers Institute for Medical Research v1

2021 ◽  
Author(s):  
Shane C. Miller ◽  
Diana P Baumann ◽  
M. Shane Merryman
Keyword(s):  
North America ◽  
Medical Research ◽  
Environmental Conditions ◽  
Atlantic Coast ◽  
Model Organism ◽  
Environmental Parameters ◽  
Sea Anemone ◽  
Nematostella Vectensis ◽  
Research Results ◽  
Impact Research

The starlet sea anemone (Nematostella vectensis) is an emerging model organism, and we have maintained a colony at the Stowers Institute since 2007. Nematostella are known as a simple sea anemone, related to other cnidarians such as jellyfish and corals. Native to estuarine environments across the Atlantic coast of North America, from Novia Scotia to Florida, they encounter a variety of environmental conditions (e.g., temperature, salinity). Acknowledging that husbandry conditions and environmental parameters can impact research results we provide information about the housing, nutrition, maintenance, and health for our colony of Nematostella. This information will be applicable to any Nematostella housed in the facility in 2021.

scholarly journals Development and epithelial organisation of muscle cells in the sea anemone Nematostella vectensis

2014 ◽  
Vol 11 (1) ◽  
pp. 44 ◽  
Author(s):  
Stefan M Jahnel ◽  
Manfred Walzl ◽  
Ulrich Technau
Keyword(s):  
Muscle Cells ◽  
Sea Anemone ◽  
Nematostella Vectensis

scholarly journals Germ-layer commitment and axis formation in sea anemone embryonic cell aggregates

2018 ◽  
Vol 115 (8) ◽  
pp. 1813-1818 ◽  
Author(s):  
Anastasia Kirillova ◽  
Grigory Genikhovich ◽  
Ekaterina Pukhlyakova ◽  
Adrien Demilly ◽  
Yulia Kraus ◽  
...  
Keyword(s):  
Normal Development ◽  
Developmental Trajectories ◽  
Embryonic Cell ◽  
Sea Anemone ◽  
Developmental Trajectory ◽  
Nematostella Vectensis ◽  
Axis Formation ◽  
Cell Aggregates ◽  
Embryonic Cells ◽  
Developmental Context

Robust morphogenetic events are pivotal for animal embryogenesis. However, comparison of the modes of development of different members of a phylum suggests that the spectrum of developmental trajectories accessible for a species might be far broader than can be concluded from the observation of normal development. Here, by using a combination of microsurgery and transgenic reporter gene expression, we show that, facing a new developmental context, the aggregates of dissociated embryonic cells of the sea anemone Nematostella vectensis take an alternative developmental trajectory. The self-organizing aggregates rely on Wnt signals produced by the cells of the original blastopore lip organizer to form body axes but employ morphogenetic events typical for normal development of distantly related cnidarians to re-establish the germ layers. The reaggregated cells show enormous plasticity including the capacity of the ectodermal cells to convert into endoderm. Our results suggest that new developmental trajectories may evolve relatively easily when highly plastic embryonic cells face new constraints.

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