Determination of site-specific glycan heterogeneity on glycoproteins

2012 ◽  
Vol 7 (7) ◽  
pp. 1285-1298 ◽  
Author(s):  
Daniel Kolarich ◽  
Pia H Jensen ◽  
Friedrich Altmann ◽  
Nicolle H Packer
Keyword(s):  
Site Specific ◽  
Glycan Heterogeneity

Site-Specific pKa Determination of Selenocysteine Residues in Selenovasopressin by Using 77Se NMR Spectroscopy

2011 ◽  
Vol 50 (50) ◽  
pp. 11952-11955 ◽  
Author(s):  
Mehdi Mobli ◽  
David Morgenstern ◽  
Glenn F. King ◽  
Paul F. Alewood ◽  
Markus Muttenthaler
Keyword(s):  
Nmr Spectroscopy ◽  
Site Specific ◽  
Pka Determination ◽  
77Se Nmr Spectroscopy

Differential Isotope Labeling of Glycopeptides for Accurate Determination of Differences in Site-Specific Glycosylation

2015 ◽  
Vol 15 (1) ◽  
pp. 326-331 ◽  
Author(s):  
Martin Pabst ◽  
Iva Benešová ◽  
Stephan R. Fagerer ◽  
Mathias Jacobsen ◽  
Klaus Eyer ◽  
...  
Keyword(s):  
Isotope Labeling ◽  
Accurate Determination ◽  
Site Specific

Determination of Site-Specific Phosphorylation Ratios in Proteins with Targeted Mass Spectrometry

2018 ◽  
Vol 17 (4) ◽  
pp. 1654-1663 ◽  
Author(s):  
Lennard J. M. Dekker ◽  
Lona Zeneyedpour ◽  
Sandor Snoeijers ◽  
Jos Joore ◽  
Sieger Leenstra ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Site Specific

Determination of Site Specific Fertilizer Requirement of Sugarcane and Intercrops in Sugarcane-based Cropping Systems

2002 ◽  
Vol 5 (2) ◽  
pp. 165-168 ◽  
Author(s):  
S.M. Bokhtiar ◽  
M.L. Kabir ◽  
M.J. Alam ◽  
M. M. Alam ◽  
M.H. Rahman
Keyword(s):  
Cropping Systems ◽  
Site Specific ◽  
Fertilizer Requirement

scholarly journals Determination of Site-Specific (Deuterium/Hydrogen) Ratios in Vanillin by 2H-NuclearMagnetic Resonance Spectrometry: Collaborative Study

2007 ◽  
Vol 90 (1) ◽  
pp. 187-195 ◽  
Author(s):  
Eric Jamin ◽  
Frédérique Martin ◽  
Gilles G Martin ◽  
I Billault ◽  
A -I Blanch-Cortès ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Collaborative Study ◽  
Official Method ◽  
Site Specific ◽  
Hydrogen Position ◽  
Nmr Spectrometry ◽  
Measuring Site ◽  
Commercial Sources

Abstract The results of collaborative study are reported for a method that determines the site-specific isotope ratios of deuterium/hydrogen (D/H)i in vanillin by deuterium-nuclear magnetic resonance (2H-NMR) spectrometry. This method allows characterization of all the main commercial sources of commercial vanillin and detection of undeclared mixtures. It is based on the fact that the amounts of deuterium at various positions in the vanillin molecule are significantly different from one source to another. Vanillin is dissolved in acetonitrile and analyzed with a high-field NMR spectrometer fitted with a deuterium probe and a fluorine lock. The proportions of isotopomers monodeuterated at each hydrogen position of the molecule are recorded, and the corresponding (D/H) ratios are determined by using a calibrated reference. Nine laboratories analyzed 5 materials supplied as blind duplicates (1 natural vanillin from vanilla beans, 2 synthetic vanillins from guaiacol, 1 semisynthetic vanillin from lignin, and a mixture of natural and synthetic vanillins). The precision of the method for measuring site-specific ratios was as follows: for (D/H)1 the within-laboratory standard deviation (sr) values ranged from 2.2 to 5.8 ppm, and the among-laboratories standard deviation (sR) values ranged from 3.6 to 5.1 ppm; for (D/H)3 the sr values ranged from 1.7 to 3.2 ppm, and the sR values ranged from 2.4 to 3.7 ppm; for (D/H)4 the sr values ranged from 2.3 to 6.2 ppm, and the sR values ranged from 2.4 to 6.4 ppm; for (D/H)5 the sr values ranged from 0.8 to 2.7 ppm, and the sR values ranged from 0.9 to 2.3 ppm. It was shown that these values allow a satisfactory discrimination between vanillin sources. Therefore, the Study Director recommends the method for adoption as a First Action Official Method by AOAC INTERNATIONAL.

Impact of site-specific fertilizing management in carbon and water footprint. The case of cotton under Mediterranean conditions

2020 ◽  
Author(s):  
Antonios Papadopoulos ◽  
Gerasimos Troyanos ◽  
Dionissios Kalivas ◽  
Maria Doula ◽  
Stamatios Kavasilis ◽  
...  
Keyword(s):  
Water Footprint ◽  
Meteorological Data ◽  
Ghg Emissions ◽  
Emission Factors ◽  
The Other ◽  
Chemical Pollution ◽  
Agricultural Practice ◽  
Site Specific ◽  
Specific Management

<p>In field homogenous application of fertilizers can be considered as a non-environment friendly agricultural practice as it ignores site specific variations of soil and plant properties. Conventional fertilizing management usually results in overfertilization guiding to burdens of the environment in terms of chemical pollution in soil-water system and Greenhouse Gas (GHG) emissions in the atmosphere. The effects are expected to be more severe in Mediterranean region under the evolving climate change. Site-specific fertilizing management on the other hand, poses a practice that is adapted to high precision spatial soil, climatic and plant conditions. In this sense, the agricultural practices are properly adjusted to the needs of the crops. The research is focused on the assessment of the impacts of conventional and site-specific management of nitrogen fertilization to carbon and water footprint at cotton cultivation in field level. The study area concerns two cotton fields in Central Greece that were monitored with the use of classical soil analytical methods and remote sensing sensors throughout a cultivation period. The monitoring process led to the delineation of the fields in different management zones needing variable fertilizing doses. Further, all conventionally applied practices were annotated concerning the last 4-year period in order to collect historical fertilizing data. In both cases (conventional and site-specific) the carbon and water (blue, green, grey) footprints of the two fields were calculated. Carbon footprint was calculated by assessing IPCC 2006 guidelines (updated in 2019) as regards direct and LULUCF emissions. For this, Tier 2 emission factors were used for the main emission categories, as these were defined by the Greek State, while for the other categories, emission factors of Tier 1 of IPCC guidelines were used. For the determination of water footprint, local meteorological data and cotton development stages concerning Greek conditions were used. The determination of the footprints was realized with the use of a software tool developed by the BalkanROAD project in the framework of INTERREG Balkan-Mediterranean 2014-2020 programme, which addresses territorial competitiveness and environment. Preliminary results show encouraging prospective for the improvement of carbon and water footprint when shifting from conventional to site-specific management.</p>

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