scholarly journals Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure

2012 ◽  
Vol 7 (4) ◽  
pp. 654-669 ◽  
Author(s):  
Xiyi Chen ◽  
Oleg Nadiarynkh ◽  
Sergey Plotnikov ◽  
Paul J Campagnola
Keyword(s):  
Quantitative Analysis ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Fibrillar Structure ◽  
Second Harmonic Generation Microscopy

scholarly journals Maturation of the Meniscal Collagen Structure Revealed by Polarization-Resolved and Directional Second Harmonic Generation Microscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Maxime Pinsard ◽  
Sheila Laverty ◽  
Hélène Richard ◽  
Julia Dubuc ◽  
Marie-Claire Schanne-Klein ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Large Field ◽  
Fibrillar Structure ◽  
Collagen Structure ◽  
Future Studies ◽  
Structural Modifications ◽  
Second Harmonic Generation Microscopy

AbstractWe report Polarization-resolved Second Harmonic Generation (P-SHG) and directional SHG (forward and backward, F/B) measurements of equine foetal and adult collagen in meniscus, over large field-of-views using sample-scanning. Large differences of collagen structure and fibril orientation with maturation are revealed, validating the potential for this novel methodology to track such changes in meniscal structure. The foetal menisci had a non-organized and more random collagen fibrillar structure when compared with adult using P-SHG. For the latter, clusters of homogeneous fibril orientation (inter-fibrillar areas) were revealed, separated by thick fibers. F/B SHG showed numerous different features in adults notably, in thick fibers compared to interfibrillar areas, unlike foetal menisci that showed similar patterns for both directions. This work confirms previous studies and improves the understanding of meniscal collagen structure and its maturation, and makes F/B and P-SHG good candidates for future studies aiming at revealing structural modifications to meniscus due to pathologies.

Selective Detection and Quantitation of Organic Molecule Crystallization by Second Harmonic Generation Microscopy

2010 ◽  
Vol 82 (13) ◽  
pp. 5425-5432 ◽  
Author(s):  
Duangporn Wanapun ◽  
Umesh S. Kestur ◽  
David J. Kissick ◽  
Garth J. Simpson ◽  
Lynne S. Taylor
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Organic Molecule ◽  
Second Harmonic ◽  
Selective Detection ◽  
Second Harmonic Generation Microscopy
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